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Basic biological characterization of feline morbillivirus.

Koide R, Sakaguchi S, Miyazawa T - J. Vet. Med. Sci. (2015)

Bottom Line: Treatment with polybrene® or trypsin which was previously used in virus isolation did not augment the virus titers.Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2 min, respectively.The biological characteristics of FmoPV reported here will be beneficial for establishing an efficient virus isolation method and will provide important information to take a measure to reduce the risk of FmoPV infection.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.

ABSTRACT
Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic nephritis, little is known about its biological characteristics. In this study, we established a quantitative assay of FmoPV by using an indirect immunofluorescence technique. Viral titers of FmoPV were determined in one week. Treatment with polybrene® or trypsin which was previously used in virus isolation did not augment the virus titers. FmoPV was notably stable at 4°C, retaining high titers for at least 12 days. Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2 min, respectively. The biological characteristics of FmoPV reported here will be beneficial for establishing an efficient virus isolation method and will provide important information to take a measure to reduce the risk of FmoPV infection.

No MeSH data available.


Related in: MedlinePlus

Determination of incubation period for obtaining the maximum titer of the stockvirus. (A) The titers of stock virus of FmoPV strain SS1 at different incubationperiods. The data are the means ± standard deviations of three experiments. (B) Greencytoplasmic fluorescence in FmoPV-inoculated CRFK cells. At 9 d.p.i., partialdetachment of cells was also observed. (C) Comparison of CPEs of FmoPV-infected CRFKcells and CDV-infected Vero cells. White bar: 200 µm.
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fig_001: Determination of incubation period for obtaining the maximum titer of the stockvirus. (A) The titers of stock virus of FmoPV strain SS1 at different incubationperiods. The data are the means ± standard deviations of three experiments. (B) Greencytoplasmic fluorescence in FmoPV-inoculated CRFK cells. At 9 d.p.i., partialdetachment of cells was also observed. (C) Comparison of CPEs of FmoPV-infected CRFKcells and CDV-infected Vero cells. White bar: 200 µm.

Mentions: Establishment of a titration method using CRFK cells: Firstly, wedetermined the optimum incubation period required for virus titration. We inoculated theserially diluted stock virus into CRFK cells, and the virus-inoculated CRFK cells wereincubated for 1, 3, 5, 7, 9 and 11 days at 37°C in a CO2 incubator. Then, wedetermined the virus titer by indirect IF assay at each incubation period. As shown in Fig. 1AFig. 1.


Basic biological characterization of feline morbillivirus.

Koide R, Sakaguchi S, Miyazawa T - J. Vet. Med. Sci. (2015)

Determination of incubation period for obtaining the maximum titer of the stockvirus. (A) The titers of stock virus of FmoPV strain SS1 at different incubationperiods. The data are the means ± standard deviations of three experiments. (B) Greencytoplasmic fluorescence in FmoPV-inoculated CRFK cells. At 9 d.p.i., partialdetachment of cells was also observed. (C) Comparison of CPEs of FmoPV-infected CRFKcells and CDV-infected Vero cells. White bar: 200 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4478736&req=5

fig_001: Determination of incubation period for obtaining the maximum titer of the stockvirus. (A) The titers of stock virus of FmoPV strain SS1 at different incubationperiods. The data are the means ± standard deviations of three experiments. (B) Greencytoplasmic fluorescence in FmoPV-inoculated CRFK cells. At 9 d.p.i., partialdetachment of cells was also observed. (C) Comparison of CPEs of FmoPV-infected CRFKcells and CDV-infected Vero cells. White bar: 200 µm.
Mentions: Establishment of a titration method using CRFK cells: Firstly, wedetermined the optimum incubation period required for virus titration. We inoculated theserially diluted stock virus into CRFK cells, and the virus-inoculated CRFK cells wereincubated for 1, 3, 5, 7, 9 and 11 days at 37°C in a CO2 incubator. Then, wedetermined the virus titer by indirect IF assay at each incubation period. As shown in Fig. 1AFig. 1.

Bottom Line: Treatment with polybrene® or trypsin which was previously used in virus isolation did not augment the virus titers.Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2 min, respectively.The biological characteristics of FmoPV reported here will be beneficial for establishing an efficient virus isolation method and will provide important information to take a measure to reduce the risk of FmoPV infection.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.

ABSTRACT
Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic nephritis, little is known about its biological characteristics. In this study, we established a quantitative assay of FmoPV by using an indirect immunofluorescence technique. Viral titers of FmoPV were determined in one week. Treatment with polybrene® or trypsin which was previously used in virus isolation did not augment the virus titers. FmoPV was notably stable at 4°C, retaining high titers for at least 12 days. Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2 min, respectively. The biological characteristics of FmoPV reported here will be beneficial for establishing an efficient virus isolation method and will provide important information to take a measure to reduce the risk of FmoPV infection.

No MeSH data available.


Related in: MedlinePlus