Limits...
Antinociceptive and anti-inflammatory activities of the ethanolic extract of Annona vepretorum Mart. (Annonaceae) in rodents.

Silva JC, Araújo Cde S, de Lima-Saraiva SR, de Oliveira-Junior RG, Diniz TC, Wanderley CW, Palheta-Júnior RC, Mendes RL, Guimarães AG, Quintans-Júnior LJ, Almeida JR - BMC Complement Altern Med (2015)

Bottom Line: Av-EtOH (25, 50 and 100 mg/kg, p.o) significantly reduced the number of writhing (P < 0.01) and decreased (P < 0.01) the paw licking time in both phases of the formalin test.In the hot plate and tail flick tests, this extract increased the reaction time, consequently reduced painful behavior.The effects in the formalin and hot plate tests were antagonized by naloxone.

View Article: PubMed Central - PubMed

Affiliation: Center for Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, 56.304-205, Petrolina, Pernambuco, Brazil. larbacjuliane@gmail.com.

ABSTRACT

Background: Annona vepretorum Mart. (Annonaceae) is a native tree from Caatinga (Brazilian Northeastern savanna biome), popularly known as "araticum" and "pinha da Caatinga". In this study, we investigated the effects of the crude ethanolic extract (Av-EtOH) in models of pain and inflammation in rodents.

Methods: The evaluation of antinociceptive activity was carried out by the acetic acid-induced writhing, formalin, hot plate and tail flick tests, while paw edema induced by carrageenan or histamine, and leukocyte migration to the peritoneal cavity were used for anti-inflammatory profile. Histological analyses also were carried out.

Results: Av-EtOH (25, 50 and 100 mg/kg, p.o) significantly reduced the number of writhing (P < 0.01) and decreased (P < 0.01) the paw licking time in both phases of the formalin test. In the hot plate and tail flick tests, this extract increased the reaction time, consequently reduced painful behavior. The effects in the formalin and hot plate tests were antagonized by naloxone. Av-EtOH inhibited significantly (P < 0.01) the increase in the edema volume after administration of carrageenan and histamine. In the peritonitis test, acute pre-treatment with Av-EtOH inhibited leukocyte migration. Histological analysis showed less inflammation in the groups treated with the extract when the inflammation was induced by carrageenan or histamine.

Conclusion: Thus, Av-EtOH has significant antinociceptive and anti-inflammatory properties, which are related probably with the activation of opioid receptors and inhibition of release of mediators of the inflammatory process. This specie is a potential target for drug discovery.

No MeSH data available.


Related in: MedlinePlus

Effect of ethanolic extract of A. vepretorum (Av-EtOH) on histamine-induced hind paw edema in mice. Values are mean ± S.E.M.; *P < 0.05, significantly different from control; unpaired Student’s t-test (n = 6, per group)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4478715&req=5

Fig6: Effect of ethanolic extract of A. vepretorum (Av-EtOH) on histamine-induced hind paw edema in mice. Values are mean ± S.E.M.; *P < 0.05, significantly different from control; unpaired Student’s t-test (n = 6, per group)

Mentions: The inflammatory edema induced by histamine was significantly attenuated by Av-EtOH 100 mg/kg, p.o. in 30 min and 60 min (P < 0.05), as shown in Fig. 6.Fig. 6


Antinociceptive and anti-inflammatory activities of the ethanolic extract of Annona vepretorum Mart. (Annonaceae) in rodents.

Silva JC, Araújo Cde S, de Lima-Saraiva SR, de Oliveira-Junior RG, Diniz TC, Wanderley CW, Palheta-Júnior RC, Mendes RL, Guimarães AG, Quintans-Júnior LJ, Almeida JR - BMC Complement Altern Med (2015)

Effect of ethanolic extract of A. vepretorum (Av-EtOH) on histamine-induced hind paw edema in mice. Values are mean ± S.E.M.; *P < 0.05, significantly different from control; unpaired Student’s t-test (n = 6, per group)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4478715&req=5

Fig6: Effect of ethanolic extract of A. vepretorum (Av-EtOH) on histamine-induced hind paw edema in mice. Values are mean ± S.E.M.; *P < 0.05, significantly different from control; unpaired Student’s t-test (n = 6, per group)
Mentions: The inflammatory edema induced by histamine was significantly attenuated by Av-EtOH 100 mg/kg, p.o. in 30 min and 60 min (P < 0.05), as shown in Fig. 6.Fig. 6

Bottom Line: Av-EtOH (25, 50 and 100 mg/kg, p.o) significantly reduced the number of writhing (P < 0.01) and decreased (P < 0.01) the paw licking time in both phases of the formalin test.In the hot plate and tail flick tests, this extract increased the reaction time, consequently reduced painful behavior.The effects in the formalin and hot plate tests were antagonized by naloxone.

View Article: PubMed Central - PubMed

Affiliation: Center for Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, 56.304-205, Petrolina, Pernambuco, Brazil. larbacjuliane@gmail.com.

ABSTRACT

Background: Annona vepretorum Mart. (Annonaceae) is a native tree from Caatinga (Brazilian Northeastern savanna biome), popularly known as "araticum" and "pinha da Caatinga". In this study, we investigated the effects of the crude ethanolic extract (Av-EtOH) in models of pain and inflammation in rodents.

Methods: The evaluation of antinociceptive activity was carried out by the acetic acid-induced writhing, formalin, hot plate and tail flick tests, while paw edema induced by carrageenan or histamine, and leukocyte migration to the peritoneal cavity were used for anti-inflammatory profile. Histological analyses also were carried out.

Results: Av-EtOH (25, 50 and 100 mg/kg, p.o) significantly reduced the number of writhing (P < 0.01) and decreased (P < 0.01) the paw licking time in both phases of the formalin test. In the hot plate and tail flick tests, this extract increased the reaction time, consequently reduced painful behavior. The effects in the formalin and hot plate tests were antagonized by naloxone. Av-EtOH inhibited significantly (P < 0.01) the increase in the edema volume after administration of carrageenan and histamine. In the peritonitis test, acute pre-treatment with Av-EtOH inhibited leukocyte migration. Histological analysis showed less inflammation in the groups treated with the extract when the inflammation was induced by carrageenan or histamine.

Conclusion: Thus, Av-EtOH has significant antinociceptive and anti-inflammatory properties, which are related probably with the activation of opioid receptors and inhibition of release of mediators of the inflammatory process. This specie is a potential target for drug discovery.

No MeSH data available.


Related in: MedlinePlus