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CYCLoPs: A Comprehensive Database Constructed from Automated Analysis of Protein Abundance and Subcellular Localization Patterns in Saccharomyces cerevisiae.

Koh JL, Chong YT, Friesen H, Moses A, Boone C, Andrews BJ, Moffat J - G3 (Bethesda) (2015)

Bottom Line: Changes in protein subcellular localization and abundance are central to biological regulation in eukaryotic cells.Quantitative measures of protein dynamics in vivo are therefore highly useful for elucidating specific regulatory pathways.The images depict the localization and abundance dynamics of more than 4000 proteins under two chemical treatments and in a selected mutant background.

View Article: PubMed Central - PubMed

Affiliation: The Donnelly Centre, University of Toronto, Toronto, Ontario, Canada, M5S3E1.

No MeSH data available.


Related in: MedlinePlus

ImageViewer showing micrographs of a wild-type strain expressing Hxt2-GFP after growth in standard medium (left) and 300 min after treatment with rapamycin (RAP300, right).
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fig5: ImageViewer showing micrographs of a wild-type strain expressing Hxt2-GFP after growth in standard medium (left) and 300 min after treatment with rapamycin (RAP300, right).

Mentions: The Image Viewer facilitates visual inspection of pairs of micrographs. Users can toggle between 18 screens, four images per screen, and three image channels (RFP/GFP/GFP-RFP overlay). This tool is particularly useful for visual inspection of morphologic changes. For example, Figure 5 shows internalization of Hxt2, a glucose transporter, in response to rapamycin treatment. Cells in the left micrograph (from a WT screen) display morphologic patterns that define a cell-periphery localization of Hxt2, whereas most cells in the right micrograph (after 300 min of rapamycin treatment) exhibit an obvious localization to vacuole/vacuolar membrane. The Cell Viewer provides a detailed view of a specified micrograph by cropping it into individual cells (Figure 6). The position coordinates of each cell image were obtained from the cell segmentation routine in our image analysis program. The localization labels of the cell were determined using our ensemble classifiers.


CYCLoPs: A Comprehensive Database Constructed from Automated Analysis of Protein Abundance and Subcellular Localization Patterns in Saccharomyces cerevisiae.

Koh JL, Chong YT, Friesen H, Moses A, Boone C, Andrews BJ, Moffat J - G3 (Bethesda) (2015)

ImageViewer showing micrographs of a wild-type strain expressing Hxt2-GFP after growth in standard medium (left) and 300 min after treatment with rapamycin (RAP300, right).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4478550&req=5

fig5: ImageViewer showing micrographs of a wild-type strain expressing Hxt2-GFP after growth in standard medium (left) and 300 min after treatment with rapamycin (RAP300, right).
Mentions: The Image Viewer facilitates visual inspection of pairs of micrographs. Users can toggle between 18 screens, four images per screen, and three image channels (RFP/GFP/GFP-RFP overlay). This tool is particularly useful for visual inspection of morphologic changes. For example, Figure 5 shows internalization of Hxt2, a glucose transporter, in response to rapamycin treatment. Cells in the left micrograph (from a WT screen) display morphologic patterns that define a cell-periphery localization of Hxt2, whereas most cells in the right micrograph (after 300 min of rapamycin treatment) exhibit an obvious localization to vacuole/vacuolar membrane. The Cell Viewer provides a detailed view of a specified micrograph by cropping it into individual cells (Figure 6). The position coordinates of each cell image were obtained from the cell segmentation routine in our image analysis program. The localization labels of the cell were determined using our ensemble classifiers.

Bottom Line: Changes in protein subcellular localization and abundance are central to biological regulation in eukaryotic cells.Quantitative measures of protein dynamics in vivo are therefore highly useful for elucidating specific regulatory pathways.The images depict the localization and abundance dynamics of more than 4000 proteins under two chemical treatments and in a selected mutant background.

View Article: PubMed Central - PubMed

Affiliation: The Donnelly Centre, University of Toronto, Toronto, Ontario, Canada, M5S3E1.

No MeSH data available.


Related in: MedlinePlus