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Genome-Wide Association Study on Resistance to Stalk Rot Diseases in Grain Sorghum.

Adeyanju A, Little C, Yu J, Tesso T - G3 (Bethesda) (2015)

Bottom Line: However, each associated SNP had relatively small effect on the traits, accounting for 19-30% of phenotypic variation.Linkage disequilibrium analyses suggest that significant SNPs are genetically independent.Estimation of frequencies of associated alleles revealed that durra and caudatum subpopulations were enriched for resistant alleles, but the results suggest complex molecular mechanisms underlying resistance to both pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Agronomy, Kansas State University, Manhattan, Kansas 66506.

No MeSH data available.


Related in: MedlinePlus

Field procedure for administering the treatments and scoring phenotypic data. Artificial inoculation with Macrophomina phaseolina using the toothpick method (A). Longitudinally split stalk of resistant (B) and susceptible (C) genotypes showing contrasting tissue lesion and stalk disintegration by the pathogen.
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fig1: Field procedure for administering the treatments and scoring phenotypic data. Artificial inoculation with Macrophomina phaseolina using the toothpick method (A). Longitudinally split stalk of resistant (B) and susceptible (C) genotypes showing contrasting tissue lesion and stalk disintegration by the pathogen.

Mentions: A pure culture of M. phaseolina was also provided by Dr. Chris Little. The pathogen was then subcultured into several fresh potato dextrose agar plates into which sterile toothpicks were inserted. The culture was incubated at 30° for 2 wk and the infested toothpicks were directly used for inoculation. Again, on d 14 after flowering, the remaining three blue-tagged plants in each plot were inoculated by inserting infested toothpicks into the basal stalks (approximately 10 cm above soil surface) through holes made with a sterilized needle (Figure 1A).


Genome-Wide Association Study on Resistance to Stalk Rot Diseases in Grain Sorghum.

Adeyanju A, Little C, Yu J, Tesso T - G3 (Bethesda) (2015)

Field procedure for administering the treatments and scoring phenotypic data. Artificial inoculation with Macrophomina phaseolina using the toothpick method (A). Longitudinally split stalk of resistant (B) and susceptible (C) genotypes showing contrasting tissue lesion and stalk disintegration by the pathogen.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4478546&req=5

fig1: Field procedure for administering the treatments and scoring phenotypic data. Artificial inoculation with Macrophomina phaseolina using the toothpick method (A). Longitudinally split stalk of resistant (B) and susceptible (C) genotypes showing contrasting tissue lesion and stalk disintegration by the pathogen.
Mentions: A pure culture of M. phaseolina was also provided by Dr. Chris Little. The pathogen was then subcultured into several fresh potato dextrose agar plates into which sterile toothpicks were inserted. The culture was incubated at 30° for 2 wk and the infested toothpicks were directly used for inoculation. Again, on d 14 after flowering, the remaining three blue-tagged plants in each plot were inoculated by inserting infested toothpicks into the basal stalks (approximately 10 cm above soil surface) through holes made with a sterilized needle (Figure 1A).

Bottom Line: However, each associated SNP had relatively small effect on the traits, accounting for 19-30% of phenotypic variation.Linkage disequilibrium analyses suggest that significant SNPs are genetically independent.Estimation of frequencies of associated alleles revealed that durra and caudatum subpopulations were enriched for resistant alleles, but the results suggest complex molecular mechanisms underlying resistance to both pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Agronomy, Kansas State University, Manhattan, Kansas 66506.

No MeSH data available.


Related in: MedlinePlus