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Expellency, anti-feeding and speed of kill of a dinotefuran-permethrin-pyriproxyfen spot-on (Vectra®3D) in dogs weekly challenged with adult fleas (Ctenocephalides felis) for 1 month-comparison to a spinosad tablet (Comfortis®).

Varloud M, Fourie JJ, Blagburn BL, Deflandre A - Parasitol. Res. (2015)

Bottom Line: DPP exhibited a significantly higher and sustained speed of kill than S.The average insecticidal efficacy was 86 ± 8.8 and 95.3 ± 2.1 % with DPP, whereas it was only 33.7 ± 19.9 and 57.6 ± 18.6 % with S at respectively 1 and 4 h after weekly reinfestations.The DPP combination significantly inhibited the feeding of fleas (89 % reduction) up to onset of flea mortality for 1-month post-treatment.

View Article: PubMed Central - PubMed

Affiliation: Ceva Santé Animale S. A, 10 Avenue de la Ballastière, 33500, Libourne, France, marie.varloud@ceva.com.

ABSTRACT
This study was designed to compare the efficacy of two ectoparasiticides against adult fleas on dogs: a topical (DPP, dinotefuran-permethrin-pyriproxyfen) and a systemic (S, spinosad). Dogs (n = 48; 10.21-22.86 kg BW) were allocated to six groups of eight dogs each (C1, C4, DPP1, DPP4, S1, S4). Dogs in the treated groups were administered a topical (3.6 mL of DPP) or a tablet (665 or 1040 mg of S) on day 0. Infestations with 100 unfed fleas (Ctenocephalides felis) occurred on days -6, -1, 2, 7, 14, 21 and 28. An additional untreated group (QC, n = 6) was involved to evaluate the flea-anti-feeding efficacy. These dogs were infested once with 150 fleas prior to combing of at least 50 live fleas from each dog 5 or 10 min after infestation. In the treated group, dislodged dead and moribund fleas were collected from dogs 5, 10, 15 and 60 min (DPP1, S1) or 5, 10, 30 and 240 min (DPP4, S4) post-treatment and subsequent flea infestations on pans placed underneath the cages. Fleas were counted and removed from dogs by combing 1 (C1, DPP1, S1) or 4 h (C4, DPP4, S4) post-treatment and subsequent infestations. Quantitative PCR analysis of the canine cytochrome b gene was conducted on dislodged fleas collected from treated and control (QC) dogs 5 and 10 min after post-treatment infestations. The number of gene copies was used as a marker of blood volume ingested by fleas. Dislodgeability and insecticidal efficacy were calculated using arithmetic means. A rapid onset of killing was observed for DPP with 12.7 % of dead and moribund fleas being dislodged in average from dogs as soon as 5 min after infestation. DPP exhibited a significantly higher and sustained speed of kill than S. The average insecticidal efficacy was 86 ± 8.8 and 95.3 ± 2.1 % with DPP, whereas it was only 33.7 ± 19.9 and 57.6 ± 18.6 % with S at respectively 1 and 4 h after weekly reinfestations. The DPP combination significantly inhibited the feeding of fleas (89 % reduction) up to onset of flea mortality for 1-month post-treatment.

No MeSH data available.


Related in: MedlinePlus

Weekly assessment over 1 month of the geometric mean number of canine cytochrome b gene copies in fleas dislodged from dogs treated with dinotefuran-permethrin-pyriproxyfen (DPP) on day 0 and compared to fleas removed from untreated dogs 5 or 10 min after infestation
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Related In: Results  -  Collection


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Fig2: Weekly assessment over 1 month of the geometric mean number of canine cytochrome b gene copies in fleas dislodged from dogs treated with dinotefuran-permethrin-pyriproxyfen (DPP) on day 0 and compared to fleas removed from untreated dogs 5 or 10 min after infestation

Mentions: In CQ dogs, the GM number of canine cytochrome b gene copies per flea was 235.9 and 304.6 as measured from fleas combed 5 or 10 min after infestation, respectively (Table 6). In the DPP-treated dogs, the GM number of cytochrome b gene copies per flea ranged between weekly challenges from 6.4 to 55.5 in fleas collected 5 min after infestation and from 11.4 to 75.9 in fleas collected 10 min after infestation (Fig. 2). The average feeding inhibition was 89.3 and 86.2 % when measured 5 and 10 min after infestation of the DPP-treated dogs, respectively. In the S-treated dogs only 0.8 ± 0.4 and 1.5 ± 1.0 fleas were dislodged in average 5 and 10 min after reinfestations. It was therefore not possible to perform a reliable assessment of the quantity of blood taken by fleas from these dogs.Table 6


Expellency, anti-feeding and speed of kill of a dinotefuran-permethrin-pyriproxyfen spot-on (Vectra®3D) in dogs weekly challenged with adult fleas (Ctenocephalides felis) for 1 month-comparison to a spinosad tablet (Comfortis®).

Varloud M, Fourie JJ, Blagburn BL, Deflandre A - Parasitol. Res. (2015)

Weekly assessment over 1 month of the geometric mean number of canine cytochrome b gene copies in fleas dislodged from dogs treated with dinotefuran-permethrin-pyriproxyfen (DPP) on day 0 and compared to fleas removed from untreated dogs 5 or 10 min after infestation
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4478438&req=5

Fig2: Weekly assessment over 1 month of the geometric mean number of canine cytochrome b gene copies in fleas dislodged from dogs treated with dinotefuran-permethrin-pyriproxyfen (DPP) on day 0 and compared to fleas removed from untreated dogs 5 or 10 min after infestation
Mentions: In CQ dogs, the GM number of canine cytochrome b gene copies per flea was 235.9 and 304.6 as measured from fleas combed 5 or 10 min after infestation, respectively (Table 6). In the DPP-treated dogs, the GM number of cytochrome b gene copies per flea ranged between weekly challenges from 6.4 to 55.5 in fleas collected 5 min after infestation and from 11.4 to 75.9 in fleas collected 10 min after infestation (Fig. 2). The average feeding inhibition was 89.3 and 86.2 % when measured 5 and 10 min after infestation of the DPP-treated dogs, respectively. In the S-treated dogs only 0.8 ± 0.4 and 1.5 ± 1.0 fleas were dislodged in average 5 and 10 min after reinfestations. It was therefore not possible to perform a reliable assessment of the quantity of blood taken by fleas from these dogs.Table 6

Bottom Line: DPP exhibited a significantly higher and sustained speed of kill than S.The average insecticidal efficacy was 86 ± 8.8 and 95.3 ± 2.1 % with DPP, whereas it was only 33.7 ± 19.9 and 57.6 ± 18.6 % with S at respectively 1 and 4 h after weekly reinfestations.The DPP combination significantly inhibited the feeding of fleas (89 % reduction) up to onset of flea mortality for 1-month post-treatment.

View Article: PubMed Central - PubMed

Affiliation: Ceva Santé Animale S. A, 10 Avenue de la Ballastière, 33500, Libourne, France, marie.varloud@ceva.com.

ABSTRACT
This study was designed to compare the efficacy of two ectoparasiticides against adult fleas on dogs: a topical (DPP, dinotefuran-permethrin-pyriproxyfen) and a systemic (S, spinosad). Dogs (n = 48; 10.21-22.86 kg BW) were allocated to six groups of eight dogs each (C1, C4, DPP1, DPP4, S1, S4). Dogs in the treated groups were administered a topical (3.6 mL of DPP) or a tablet (665 or 1040 mg of S) on day 0. Infestations with 100 unfed fleas (Ctenocephalides felis) occurred on days -6, -1, 2, 7, 14, 21 and 28. An additional untreated group (QC, n = 6) was involved to evaluate the flea-anti-feeding efficacy. These dogs were infested once with 150 fleas prior to combing of at least 50 live fleas from each dog 5 or 10 min after infestation. In the treated group, dislodged dead and moribund fleas were collected from dogs 5, 10, 15 and 60 min (DPP1, S1) or 5, 10, 30 and 240 min (DPP4, S4) post-treatment and subsequent flea infestations on pans placed underneath the cages. Fleas were counted and removed from dogs by combing 1 (C1, DPP1, S1) or 4 h (C4, DPP4, S4) post-treatment and subsequent infestations. Quantitative PCR analysis of the canine cytochrome b gene was conducted on dislodged fleas collected from treated and control (QC) dogs 5 and 10 min after post-treatment infestations. The number of gene copies was used as a marker of blood volume ingested by fleas. Dislodgeability and insecticidal efficacy were calculated using arithmetic means. A rapid onset of killing was observed for DPP with 12.7 % of dead and moribund fleas being dislodged in average from dogs as soon as 5 min after infestation. DPP exhibited a significantly higher and sustained speed of kill than S. The average insecticidal efficacy was 86 ± 8.8 and 95.3 ± 2.1 % with DPP, whereas it was only 33.7 ± 19.9 and 57.6 ± 18.6 % with S at respectively 1 and 4 h after weekly reinfestations. The DPP combination significantly inhibited the feeding of fleas (89 % reduction) up to onset of flea mortality for 1-month post-treatment.

No MeSH data available.


Related in: MedlinePlus