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Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India.

Sharma AK, Tyagi V, Singh S, Veer V, Agrawal OP, Sukumaran D - J Arthropod Borne Dis (2014)

Bottom Line: The mean prevalence of An. culicifacies during the study period was in the range of 8-120 per man per hour (PMH).From the study areas species B was identified from Jabalpur, Chindwara and Hoshangabad, Species C from Hoshangabad only, Species D from Narsinghpur and Khandwa and sibling species E from Mandla, Chindwara and Hoshangabad respectively.This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An. culicifacies sibling species that would enable to develop required vector control strategies.

View Article: PubMed Central - PubMed

Affiliation: Vector Management Division, Defence Research and Development Establishment, Madhya Pradesh, India.

ABSTRACT

Background: Anopheles culicifacies is an important vector of malaria in Southeast Asia, contributing to almost 70% of malaria cases in India. It exists as a complex of five morphologically indistinguishable species A, B, C, D and E with varied geographical distribution patterns. In India, 8% of the total population of Madhya Pradesh (Central India) contributes about 30% of total malaria cases, 60% of total falciparum cases and 50% of malaria deaths. An. culicifacies is the major malaria vector in this state. Vector control mainly relies on the proper identification and distribution of vector species exists in a particular area. The present study was carried out to identify the distribution of An. culicifacies sibling species in certain endemic district of Central India, Madhya Pradesh.

Methods: The An. culicifacies mosquitoes collected from the study districts were identified morphologically. The genomic DNA was isolated from the mosquitoes and subjected to Allele specific PCR targeting D3 domain of 28S ribosomal DNA.

Results: The mean prevalence of An. culicifacies during the study period was in the range of 8-120 per man per hour (PMH). From the study areas species B was identified from Jabalpur, Chindwara and Hoshangabad, Species C from Hoshangabad only, Species D from Narsinghpur and Khandwa and sibling species E from Mandla, Chindwara and Hoshangabad respectively.

Conclusion: This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An. culicifacies sibling species that would enable to develop required vector control strategies.

No MeSH data available.


Related in: MedlinePlus

D3-PCR showing the different bands to differentiate A/D and B/C/E sibling species of An. culicifacies
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Figure 2: D3-PCR showing the different bands to differentiate A/D and B/C/E sibling species of An. culicifacies

Mentions: The AS-PCR assay using different primers, the A/D specific primer (ACA) in conjunction with D3B produces 313 bp amplification product and B/C/E-specific primer (ACB) forms 133 bp product with D3A. Additionally, the external primers D3A and D3B form common product in all the samples with 382bp products in species A and D whereas 385bp in species B/C/E serving as positive control (Fig. 2). For further distinguishing the sibling species in A/D and B/C/E individually, a total of seven primers of which three primers ADF, ADR, and DF were used in the AD-PCR assay differentiating sibling species A from D, and the other four set of primers BCEF, BCR, CR and ER were used in the BCE-PCR assay differentiating sibling species B, C and E from each other. In AD-PCR, the sibling species A and D produced the bands of 359 bp for D species and 359 bp and 166 bp for sibling species A. On the other hand in BCE-PCR, the products are 248 bp for B, 248 bp and 95 bp for C and 248 and 178 for sibling species E respectively (Fig. 3).


Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India.

Sharma AK, Tyagi V, Singh S, Veer V, Agrawal OP, Sukumaran D - J Arthropod Borne Dis (2014)

D3-PCR showing the different bands to differentiate A/D and B/C/E sibling species of An. culicifacies
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4478430&req=5

Figure 2: D3-PCR showing the different bands to differentiate A/D and B/C/E sibling species of An. culicifacies
Mentions: The AS-PCR assay using different primers, the A/D specific primer (ACA) in conjunction with D3B produces 313 bp amplification product and B/C/E-specific primer (ACB) forms 133 bp product with D3A. Additionally, the external primers D3A and D3B form common product in all the samples with 382bp products in species A and D whereas 385bp in species B/C/E serving as positive control (Fig. 2). For further distinguishing the sibling species in A/D and B/C/E individually, a total of seven primers of which three primers ADF, ADR, and DF were used in the AD-PCR assay differentiating sibling species A from D, and the other four set of primers BCEF, BCR, CR and ER were used in the BCE-PCR assay differentiating sibling species B, C and E from each other. In AD-PCR, the sibling species A and D produced the bands of 359 bp for D species and 359 bp and 166 bp for sibling species A. On the other hand in BCE-PCR, the products are 248 bp for B, 248 bp and 95 bp for C and 248 and 178 for sibling species E respectively (Fig. 3).

Bottom Line: The mean prevalence of An. culicifacies during the study period was in the range of 8-120 per man per hour (PMH).From the study areas species B was identified from Jabalpur, Chindwara and Hoshangabad, Species C from Hoshangabad only, Species D from Narsinghpur and Khandwa and sibling species E from Mandla, Chindwara and Hoshangabad respectively.This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An. culicifacies sibling species that would enable to develop required vector control strategies.

View Article: PubMed Central - PubMed

Affiliation: Vector Management Division, Defence Research and Development Establishment, Madhya Pradesh, India.

ABSTRACT

Background: Anopheles culicifacies is an important vector of malaria in Southeast Asia, contributing to almost 70% of malaria cases in India. It exists as a complex of five morphologically indistinguishable species A, B, C, D and E with varied geographical distribution patterns. In India, 8% of the total population of Madhya Pradesh (Central India) contributes about 30% of total malaria cases, 60% of total falciparum cases and 50% of malaria deaths. An. culicifacies is the major malaria vector in this state. Vector control mainly relies on the proper identification and distribution of vector species exists in a particular area. The present study was carried out to identify the distribution of An. culicifacies sibling species in certain endemic district of Central India, Madhya Pradesh.

Methods: The An. culicifacies mosquitoes collected from the study districts were identified morphologically. The genomic DNA was isolated from the mosquitoes and subjected to Allele specific PCR targeting D3 domain of 28S ribosomal DNA.

Results: The mean prevalence of An. culicifacies during the study period was in the range of 8-120 per man per hour (PMH). From the study areas species B was identified from Jabalpur, Chindwara and Hoshangabad, Species C from Hoshangabad only, Species D from Narsinghpur and Khandwa and sibling species E from Mandla, Chindwara and Hoshangabad respectively.

Conclusion: This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An. culicifacies sibling species that would enable to develop required vector control strategies.

No MeSH data available.


Related in: MedlinePlus