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Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India.

Sharma AK, Tyagi V, Singh S, Veer V, Agrawal OP, Sukumaran D - J Arthropod Borne Dis (2014)

Bottom Line: The mean prevalence of An. culicifacies during the study period was in the range of 8-120 per man per hour (PMH).From the study areas species B was identified from Jabalpur, Chindwara and Hoshangabad, Species C from Hoshangabad only, Species D from Narsinghpur and Khandwa and sibling species E from Mandla, Chindwara and Hoshangabad respectively.This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An. culicifacies sibling species that would enable to develop required vector control strategies.

View Article: PubMed Central - PubMed

Affiliation: Vector Management Division, Defence Research and Development Establishment, Madhya Pradesh, India.

ABSTRACT

Background: Anopheles culicifacies is an important vector of malaria in Southeast Asia, contributing to almost 70% of malaria cases in India. It exists as a complex of five morphologically indistinguishable species A, B, C, D and E with varied geographical distribution patterns. In India, 8% of the total population of Madhya Pradesh (Central India) contributes about 30% of total malaria cases, 60% of total falciparum cases and 50% of malaria deaths. An. culicifacies is the major malaria vector in this state. Vector control mainly relies on the proper identification and distribution of vector species exists in a particular area. The present study was carried out to identify the distribution of An. culicifacies sibling species in certain endemic district of Central India, Madhya Pradesh.

Methods: The An. culicifacies mosquitoes collected from the study districts were identified morphologically. The genomic DNA was isolated from the mosquitoes and subjected to Allele specific PCR targeting D3 domain of 28S ribosomal DNA.

Results: The mean prevalence of An. culicifacies during the study period was in the range of 8-120 per man per hour (PMH). From the study areas species B was identified from Jabalpur, Chindwara and Hoshangabad, Species C from Hoshangabad only, Species D from Narsinghpur and Khandwa and sibling species E from Mandla, Chindwara and Hoshangabad respectively.

Conclusion: This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An. culicifacies sibling species that would enable to develop required vector control strategies.

No MeSH data available.


Related in: MedlinePlus

Map showing different collection sites in the study
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Figure 1: Map showing different collection sites in the study

Mentions: The adult Anopheline mosquito species were collected from different districts of Madhya Pradesh ie, Mandla (Dungaria village), Jabalpur (Barela village), Chindwara (Chakarpat and Chikhla villages), Hoshangabad (Dhadav and Padav villages), Narsinghpur (Chinki village) and Khandwa (Chighdhalia) (Fig. 1 and Table 1). These sites were selected on the basis as they represent the tribal belt along the streams of Narmada River and also show high incidence of malaria (Singh 2004, 2006, 2009, Sharma 2012). The collections were made during the transmission period i.e. February–March and August–September in the morning period between 0600 h to 0800 h using mouth aspirator and battery operated torch. The fed anophelines were captured at various collection sites including human dwellings, cattle sheds, mixed dwelling and random collection sites. The fed mosquitoes were captured so that F1 generation of these mosquitoes can be utilized for further use after egg lay. All adult mosquitoes were brought to the lab for their identification by using standard keys (Christopher 1933, Wattal and Kalra 1961, Das et al. 1990, Nagpal and Sharma 1995). Each representative sample was pinned as a voucher specimen and kept in laboratory as a reference collection. From these collection the An. culicifacies female were separated and allowed for egg laying and the adult emerged from them are used for further standardization and identification of mosquito sibling species using allele specific polymerase chain reaction (AS-PCR) (Goswami et al. 2006, Singh et al. 2006).


Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India.

Sharma AK, Tyagi V, Singh S, Veer V, Agrawal OP, Sukumaran D - J Arthropod Borne Dis (2014)

Map showing different collection sites in the study
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4478430&req=5

Figure 1: Map showing different collection sites in the study
Mentions: The adult Anopheline mosquito species were collected from different districts of Madhya Pradesh ie, Mandla (Dungaria village), Jabalpur (Barela village), Chindwara (Chakarpat and Chikhla villages), Hoshangabad (Dhadav and Padav villages), Narsinghpur (Chinki village) and Khandwa (Chighdhalia) (Fig. 1 and Table 1). These sites were selected on the basis as they represent the tribal belt along the streams of Narmada River and also show high incidence of malaria (Singh 2004, 2006, 2009, Sharma 2012). The collections were made during the transmission period i.e. February–March and August–September in the morning period between 0600 h to 0800 h using mouth aspirator and battery operated torch. The fed anophelines were captured at various collection sites including human dwellings, cattle sheds, mixed dwelling and random collection sites. The fed mosquitoes were captured so that F1 generation of these mosquitoes can be utilized for further use after egg lay. All adult mosquitoes were brought to the lab for their identification by using standard keys (Christopher 1933, Wattal and Kalra 1961, Das et al. 1990, Nagpal and Sharma 1995). Each representative sample was pinned as a voucher specimen and kept in laboratory as a reference collection. From these collection the An. culicifacies female were separated and allowed for egg laying and the adult emerged from them are used for further standardization and identification of mosquito sibling species using allele specific polymerase chain reaction (AS-PCR) (Goswami et al. 2006, Singh et al. 2006).

Bottom Line: The mean prevalence of An. culicifacies during the study period was in the range of 8-120 per man per hour (PMH).From the study areas species B was identified from Jabalpur, Chindwara and Hoshangabad, Species C from Hoshangabad only, Species D from Narsinghpur and Khandwa and sibling species E from Mandla, Chindwara and Hoshangabad respectively.This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An. culicifacies sibling species that would enable to develop required vector control strategies.

View Article: PubMed Central - PubMed

Affiliation: Vector Management Division, Defence Research and Development Establishment, Madhya Pradesh, India.

ABSTRACT

Background: Anopheles culicifacies is an important vector of malaria in Southeast Asia, contributing to almost 70% of malaria cases in India. It exists as a complex of five morphologically indistinguishable species A, B, C, D and E with varied geographical distribution patterns. In India, 8% of the total population of Madhya Pradesh (Central India) contributes about 30% of total malaria cases, 60% of total falciparum cases and 50% of malaria deaths. An. culicifacies is the major malaria vector in this state. Vector control mainly relies on the proper identification and distribution of vector species exists in a particular area. The present study was carried out to identify the distribution of An. culicifacies sibling species in certain endemic district of Central India, Madhya Pradesh.

Methods: The An. culicifacies mosquitoes collected from the study districts were identified morphologically. The genomic DNA was isolated from the mosquitoes and subjected to Allele specific PCR targeting D3 domain of 28S ribosomal DNA.

Results: The mean prevalence of An. culicifacies during the study period was in the range of 8-120 per man per hour (PMH). From the study areas species B was identified from Jabalpur, Chindwara and Hoshangabad, Species C from Hoshangabad only, Species D from Narsinghpur and Khandwa and sibling species E from Mandla, Chindwara and Hoshangabad respectively.

Conclusion: This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An. culicifacies sibling species that would enable to develop required vector control strategies.

No MeSH data available.


Related in: MedlinePlus