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Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran.

Zahraei-Ramazani A, Kumar D, Mirhendi H, Sundar S, Mishra R, Moin-Vaziri V, Soleimani H, Shirzadi MR, Jafari R, Hanafi-Bojd AA, Shahraky SH, Yaghoobi-Ershadi MR - J Arthropod Borne Dis (2014)

Bottom Line: The positions of three females and the males in the UPGMA tree are correct and the similarities among them confirm our results.The branches of each species are not genetically distinct which justify the overlapping morphological characters among them.Molecular sequencing of Cytb-mtDNA haplotypes can be used for female identification for different species of subgenus Adlerius in Iran.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Female sand flies of subgenus Adlerius are considered as probable vectors of visceral leishmaniasis in Iran. The objective of this study was to determine the morphological and genotypic variations in the populations of this subgenus in the country.

Methods: Sand flies collected using sticky traps from 17 provinces during 2008-2010. The morphometric measurements were conducted with an Ocular Micrometer. Data was analyzed by SPSS. The Cytb gene was used to estimate population genetic diversity and identify the female specimens. UPGMA phenetic tree was used for DNA haplotypes of Cytb gene.

Results: Six species of subgenus Adlerius identified from which one species, P. (Adlerius) kabulensis, is new record. The identification key is provided for males. Results revealed the molecular systematic in the species of subgenus Adlerius and determine the relationship of three females of P. comatus, P. balcanicus and P. halepensis.

Conclusion: The positions of three females and the males in the UPGMA tree are correct and the similarities among them confirm our results. The branches of each species are not genetically distinct which justify the overlapping morphological characters among them. Molecular sequencing of Cytb-mtDNA haplotypes can be used for female identification for different species of subgenus Adlerius in Iran.

No MeSH data available.


Related in: MedlinePlus

Neighbor-joining tree for DNA haplotypes of Cytb-mtDNA of the subgenus Adlerius sand flies species
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Related In: Results  -  Collection


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Figure 4: Neighbor-joining tree for DNA haplotypes of Cytb-mtDNA of the subgenus Adlerius sand flies species

Mentions: The sequence result of the length 550 bp of Cytb gene shows that 5 male species: P. halepensis, P. brevis, P. kabulensis, P. comatus and P. balcanicus are in the areas of our study. Also two male specimens: P. cf. balcanicus and P. cf. longiductus which are close to P. balcanicus and P. longiductus are on neighbor-joining tree (Fig. 4). The specimens of P. halepensis and P. brevis are in independent and different branches in the tree, but other species are close together in one branch. P. comatus and the female AZV8 specimen are in the same branch, which shows that they are the same species. Also the branch of female SHC11 specimen is near the branch of AZV4 and AZV5, which shows that females of this species are close to these specimens. The branches of two P. balcanicus specimens i.e. Ash10 and AZV11 are close together and P. kabulensis is in a different branch. The position in the phenetic tree of the 3 females species and the male species are correct and the similarities among the males and females confirm our above results (Fig. 4).


Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran.

Zahraei-Ramazani A, Kumar D, Mirhendi H, Sundar S, Mishra R, Moin-Vaziri V, Soleimani H, Shirzadi MR, Jafari R, Hanafi-Bojd AA, Shahraky SH, Yaghoobi-Ershadi MR - J Arthropod Borne Dis (2014)

Neighbor-joining tree for DNA haplotypes of Cytb-mtDNA of the subgenus Adlerius sand flies species
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4478421&req=5

Figure 4: Neighbor-joining tree for DNA haplotypes of Cytb-mtDNA of the subgenus Adlerius sand flies species
Mentions: The sequence result of the length 550 bp of Cytb gene shows that 5 male species: P. halepensis, P. brevis, P. kabulensis, P. comatus and P. balcanicus are in the areas of our study. Also two male specimens: P. cf. balcanicus and P. cf. longiductus which are close to P. balcanicus and P. longiductus are on neighbor-joining tree (Fig. 4). The specimens of P. halepensis and P. brevis are in independent and different branches in the tree, but other species are close together in one branch. P. comatus and the female AZV8 specimen are in the same branch, which shows that they are the same species. Also the branch of female SHC11 specimen is near the branch of AZV4 and AZV5, which shows that females of this species are close to these specimens. The branches of two P. balcanicus specimens i.e. Ash10 and AZV11 are close together and P. kabulensis is in a different branch. The position in the phenetic tree of the 3 females species and the male species are correct and the similarities among the males and females confirm our above results (Fig. 4).

Bottom Line: The positions of three females and the males in the UPGMA tree are correct and the similarities among them confirm our results.The branches of each species are not genetically distinct which justify the overlapping morphological characters among them.Molecular sequencing of Cytb-mtDNA haplotypes can be used for female identification for different species of subgenus Adlerius in Iran.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Female sand flies of subgenus Adlerius are considered as probable vectors of visceral leishmaniasis in Iran. The objective of this study was to determine the morphological and genotypic variations in the populations of this subgenus in the country.

Methods: Sand flies collected using sticky traps from 17 provinces during 2008-2010. The morphometric measurements were conducted with an Ocular Micrometer. Data was analyzed by SPSS. The Cytb gene was used to estimate population genetic diversity and identify the female specimens. UPGMA phenetic tree was used for DNA haplotypes of Cytb gene.

Results: Six species of subgenus Adlerius identified from which one species, P. (Adlerius) kabulensis, is new record. The identification key is provided for males. Results revealed the molecular systematic in the species of subgenus Adlerius and determine the relationship of three females of P. comatus, P. balcanicus and P. halepensis.

Conclusion: The positions of three females and the males in the UPGMA tree are correct and the similarities among them confirm our results. The branches of each species are not genetically distinct which justify the overlapping morphological characters among them. Molecular sequencing of Cytb-mtDNA haplotypes can be used for female identification for different species of subgenus Adlerius in Iran.

No MeSH data available.


Related in: MedlinePlus