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Rice stripe1-2 and stripe1-3 Mutants Encoding the Small Subunit of Ribonucleotide Reductase Are Temperature Sensitive and Are Required for Chlorophyll Biosynthesis.

Chen X, Zhu L, Xin L, Du K, Ran X, Cui X, Xiang Q, Zhang H, Xu P, Wu X - PLoS ONE (2015)

Bottom Line: The mutants were sensitive to temperature, and their chlorophyll content increased with the increase in temperature; however, they did not revert to normal green leaf phenotype under field conditions.The mutant st1-2 showed loosely arranged thylakoid lamellar structure as compared with wild-type (WT) plants.Real-time PCR analysis showed that the expression levels associated with chlorophyll biosynthetic pathway and photosynthesis were affected in st1-2 and st1-3 at different temperatures and different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.

ABSTRACT
We induced mutants, stripe1-2 (st1-2) and stripe1-3 (st1-3), from rice (Oryza sativa L.) Indica 9311 using Ethyl methanesulfonate (EMS). Both st1-2 and st1-3 mutants encoded the small subunit of ribonucleotide reductase 1 (RNRS1), differed in the location of the mutated base, and displayed white-stripe from the L2 stage through maturity. The mutants were sensitive to temperature, and their chlorophyll content increased with the increase in temperature; however, they did not revert to normal green leaf phenotype under field conditions. The mutant st1-2 showed loosely arranged thylakoid lamellar structure as compared with wild-type (WT) plants. Contrastingly, st1-3 displayed normal thylakoid lamellar structure, good agronomic traits, and higher yield than st1-2 but lower yield than WT. Three-dimensional structure prediction for RNRS1 indicated that the mutation in Val-171 residue in st1-2 influenced the connection of RNRS1 to iron, causing abnormal development of chloroplasts. Real-time PCR analysis showed that the expression levels associated with chlorophyll biosynthetic pathway and photosynthesis were affected in st1-2 and st1-3 at different temperatures and different developmental stages.

No MeSH data available.


Related in: MedlinePlus

Transcription analysis for plastid-nuclear genes involved in chlorophyll biosynthesis affected indirectly between WT and mutants by different temperature dealt.L expresses light condition; D expresses dark condition.
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pone.0130172.g010: Transcription analysis for plastid-nuclear genes involved in chlorophyll biosynthesis affected indirectly between WT and mutants by different temperature dealt.L expresses light condition; D expresses dark condition.

Mentions: The temperature sensitivity of different mutants was studied by growing the mutants in different temperature conditions. Both st1-2 and st1-3 all showed white stripes starting at stage L2. We compared the mutant plant growth with that of wild type before conducting further experiments. The mutants st1-2 and st1-3 showed retarded growth throughout their development (Fig 1C and 1D). Temperature studies in RNR showed that the expression of two smaller subunits was highly variable in response to temperature changes as compared with larger subunits (Fig 10A). The small subunits RNRS1 were up-regulated ten times in st1-2 plants at L20/D16, L26/D22 and L30/D26 as compared with WT (Fig 10A). By contrast, RNRS2 in st1-2 mainly was significantly up-regulated at L26/D22 and L30/D26 as compared with WT. However, expression of RNRS1 subunit in st1-3 was significantly less than in st1-2, but the expression of RNRS2 subunit was higher than that in st1-2. Therefore, we speculate that the two different mutant locations were responsible for the differential expression of the two small subunits.


Rice stripe1-2 and stripe1-3 Mutants Encoding the Small Subunit of Ribonucleotide Reductase Are Temperature Sensitive and Are Required for Chlorophyll Biosynthesis.

Chen X, Zhu L, Xin L, Du K, Ran X, Cui X, Xiang Q, Zhang H, Xu P, Wu X - PLoS ONE (2015)

Transcription analysis for plastid-nuclear genes involved in chlorophyll biosynthesis affected indirectly between WT and mutants by different temperature dealt.L expresses light condition; D expresses dark condition.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4478038&req=5

pone.0130172.g010: Transcription analysis for plastid-nuclear genes involved in chlorophyll biosynthesis affected indirectly between WT and mutants by different temperature dealt.L expresses light condition; D expresses dark condition.
Mentions: The temperature sensitivity of different mutants was studied by growing the mutants in different temperature conditions. Both st1-2 and st1-3 all showed white stripes starting at stage L2. We compared the mutant plant growth with that of wild type before conducting further experiments. The mutants st1-2 and st1-3 showed retarded growth throughout their development (Fig 1C and 1D). Temperature studies in RNR showed that the expression of two smaller subunits was highly variable in response to temperature changes as compared with larger subunits (Fig 10A). The small subunits RNRS1 were up-regulated ten times in st1-2 plants at L20/D16, L26/D22 and L30/D26 as compared with WT (Fig 10A). By contrast, RNRS2 in st1-2 mainly was significantly up-regulated at L26/D22 and L30/D26 as compared with WT. However, expression of RNRS1 subunit in st1-3 was significantly less than in st1-2, but the expression of RNRS2 subunit was higher than that in st1-2. Therefore, we speculate that the two different mutant locations were responsible for the differential expression of the two small subunits.

Bottom Line: The mutants were sensitive to temperature, and their chlorophyll content increased with the increase in temperature; however, they did not revert to normal green leaf phenotype under field conditions.The mutant st1-2 showed loosely arranged thylakoid lamellar structure as compared with wild-type (WT) plants.Real-time PCR analysis showed that the expression levels associated with chlorophyll biosynthetic pathway and photosynthesis were affected in st1-2 and st1-3 at different temperatures and different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Rice Research Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, 211 Huimin Road, 611130 Wenjiang, Sichuan, China.

ABSTRACT
We induced mutants, stripe1-2 (st1-2) and stripe1-3 (st1-3), from rice (Oryza sativa L.) Indica 9311 using Ethyl methanesulfonate (EMS). Both st1-2 and st1-3 mutants encoded the small subunit of ribonucleotide reductase 1 (RNRS1), differed in the location of the mutated base, and displayed white-stripe from the L2 stage through maturity. The mutants were sensitive to temperature, and their chlorophyll content increased with the increase in temperature; however, they did not revert to normal green leaf phenotype under field conditions. The mutant st1-2 showed loosely arranged thylakoid lamellar structure as compared with wild-type (WT) plants. Contrastingly, st1-3 displayed normal thylakoid lamellar structure, good agronomic traits, and higher yield than st1-2 but lower yield than WT. Three-dimensional structure prediction for RNRS1 indicated that the mutation in Val-171 residue in st1-2 influenced the connection of RNRS1 to iron, causing abnormal development of chloroplasts. Real-time PCR analysis showed that the expression levels associated with chlorophyll biosynthetic pathway and photosynthesis were affected in st1-2 and st1-3 at different temperatures and different developmental stages.

No MeSH data available.


Related in: MedlinePlus