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Identification of Ganglioside GM3 Molecular Species in Human Serum Associated with Risk Factors of Metabolic Syndrome.

Veillon L, Go S, Matsuyama W, Suzuki A, Nagasaki M, Yatomi Y, Inokuchi J - PLoS ONE (2015)

Bottom Line: A total of 23 GM3 molecular species were measured, of these, eight were found to be significantly elevated in individuals with visceral fat accumulation and metabolic disease, defined as the presence of hyperglycemia and dyslipidemia.The hydroxylated GM3 species were, in order of decreasing abundance, d18:1-h24:0 ≈ d18:1-h24:1 > d18:1-h22:0 » d18:1-h20:0 > d18:1-h21:0 > d18:1-h18:1.Univariate and multiple linear regression analyses were conducted using a number of clinical health variables associated with obesity, type 2 diabetes, metabolic disease, atherosclerosis and hypertension.

View Article: PubMed Central - PubMed

Affiliation: Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Japan.

ABSTRACT
Serum GM3 molecular species were quantified in 125 Japanese residents using tandem mass spectrometry multiple reaction monitoring. Individuals were categorized by the presence or absence of metabolic disease risk factors including visceral fat accumulation, hyperglycemia and dyslipidemia. A total of 23 GM3 molecular species were measured, of these, eight were found to be significantly elevated in individuals with visceral fat accumulation and metabolic disease, defined as the presence of hyperglycemia and dyslipidemia. All of the GM3 molecular species were composed of the sphingoid base sphingosine (d18:1 (Δ4)) and, interestingly, six of the eight elevated GM3 molecular species contained a hydroxylated ceramide moiety. The hydroxylated GM3 species were, in order of decreasing abundance, d18:1-h24:0 ≈ d18:1-h24:1 > d18:1-h22:0 » d18:1-h20:0 > d18:1-h21:0 > d18:1-h18:1. Univariate and multiple linear regression analyses were conducted using a number of clinical health variables associated with obesity, type 2 diabetes, metabolic disease, atherosclerosis and hypertension. GM3(d18:1-h24:1) was identified as the best candidate for metabolic screening, proving to be significantly correlated with intima-media thickness, used for the detection of atherosclerotic disease in humans, and a number of metabolic disease risk factors including autotaxin, LDL-c and homeostatic model assessment insulin resistance (HOMA-IR).

No MeSH data available.


Related in: MedlinePlus

Association between metabolic disease risk factors and total GM3.Correlation of total GM3 with fasting blood glucose (A), insulin (B), HOMA-IR (C), HbA1c (D), total cholesterol (E), LDL-c (F), autotaxin (G) and mean IMT (H). Spearman’s rank correlation was used to access correlation between total GM3 and metabolic disease risk factors. Correlations of fasting blood glucose, HbA1c, total cholesterol, LDL-c and mean IMT with total GM3 were deemed significant with P values below 0.05.
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pone.0129645.g004: Association between metabolic disease risk factors and total GM3.Correlation of total GM3 with fasting blood glucose (A), insulin (B), HOMA-IR (C), HbA1c (D), total cholesterol (E), LDL-c (F), autotaxin (G) and mean IMT (H). Spearman’s rank correlation was used to access correlation between total GM3 and metabolic disease risk factors. Correlations of fasting blood glucose, HbA1c, total cholesterol, LDL-c and mean IMT with total GM3 were deemed significant with P values below 0.05.

Mentions: In addition to GM3(d18:1-h24:1) it was found that total GM3 and total hydroxylated GM3 molecular species were correlated with risk factors for type 2 diabetes and CVD. Total GM3 was significant correlated with fasting blood glucose rs = 0.2793 (mg/dL, Fig 4A), Hba1c rs = 0.1820 (% glycated hemoglobin, Fig 4D), total cholesterol rs = 0.5046 (mg/dL, Fig 4E), LDL-c rs = 0.4477 (mg/dL, Fig 4F) and mean IMT rs = 0.2242 (mm, Fig 4H). While total hydroxylated GM3 molecular species were significantly correlated with fasting blood glucose rs = 0.3273 (mg/dL, Fig 5A), insulin rs = 0.2766 (mIU/L, Fig 5B), HOMA-IR rs = 0.3223 (Fig 5C), HbA1c rs = 0.2975 (% glycated hemoglobin, Fig 5D), total cholesterol rs = 0.4293 (mg/dL, Fig 5E), LDL-c rs = 0.3958 (mg/dL, Fig 5F), autotaxin rs = 0.3188 (mg/L, Fig 5G) and mean IMT rs = 0.2987 (mm, Fig 5H).


Identification of Ganglioside GM3 Molecular Species in Human Serum Associated with Risk Factors of Metabolic Syndrome.

Veillon L, Go S, Matsuyama W, Suzuki A, Nagasaki M, Yatomi Y, Inokuchi J - PLoS ONE (2015)

Association between metabolic disease risk factors and total GM3.Correlation of total GM3 with fasting blood glucose (A), insulin (B), HOMA-IR (C), HbA1c (D), total cholesterol (E), LDL-c (F), autotaxin (G) and mean IMT (H). Spearman’s rank correlation was used to access correlation between total GM3 and metabolic disease risk factors. Correlations of fasting blood glucose, HbA1c, total cholesterol, LDL-c and mean IMT with total GM3 were deemed significant with P values below 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477979&req=5

pone.0129645.g004: Association between metabolic disease risk factors and total GM3.Correlation of total GM3 with fasting blood glucose (A), insulin (B), HOMA-IR (C), HbA1c (D), total cholesterol (E), LDL-c (F), autotaxin (G) and mean IMT (H). Spearman’s rank correlation was used to access correlation between total GM3 and metabolic disease risk factors. Correlations of fasting blood glucose, HbA1c, total cholesterol, LDL-c and mean IMT with total GM3 were deemed significant with P values below 0.05.
Mentions: In addition to GM3(d18:1-h24:1) it was found that total GM3 and total hydroxylated GM3 molecular species were correlated with risk factors for type 2 diabetes and CVD. Total GM3 was significant correlated with fasting blood glucose rs = 0.2793 (mg/dL, Fig 4A), Hba1c rs = 0.1820 (% glycated hemoglobin, Fig 4D), total cholesterol rs = 0.5046 (mg/dL, Fig 4E), LDL-c rs = 0.4477 (mg/dL, Fig 4F) and mean IMT rs = 0.2242 (mm, Fig 4H). While total hydroxylated GM3 molecular species were significantly correlated with fasting blood glucose rs = 0.3273 (mg/dL, Fig 5A), insulin rs = 0.2766 (mIU/L, Fig 5B), HOMA-IR rs = 0.3223 (Fig 5C), HbA1c rs = 0.2975 (% glycated hemoglobin, Fig 5D), total cholesterol rs = 0.4293 (mg/dL, Fig 5E), LDL-c rs = 0.3958 (mg/dL, Fig 5F), autotaxin rs = 0.3188 (mg/L, Fig 5G) and mean IMT rs = 0.2987 (mm, Fig 5H).

Bottom Line: A total of 23 GM3 molecular species were measured, of these, eight were found to be significantly elevated in individuals with visceral fat accumulation and metabolic disease, defined as the presence of hyperglycemia and dyslipidemia.The hydroxylated GM3 species were, in order of decreasing abundance, d18:1-h24:0 ≈ d18:1-h24:1 > d18:1-h22:0 » d18:1-h20:0 > d18:1-h21:0 > d18:1-h18:1.Univariate and multiple linear regression analyses were conducted using a number of clinical health variables associated with obesity, type 2 diabetes, metabolic disease, atherosclerosis and hypertension.

View Article: PubMed Central - PubMed

Affiliation: Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Japan.

ABSTRACT
Serum GM3 molecular species were quantified in 125 Japanese residents using tandem mass spectrometry multiple reaction monitoring. Individuals were categorized by the presence or absence of metabolic disease risk factors including visceral fat accumulation, hyperglycemia and dyslipidemia. A total of 23 GM3 molecular species were measured, of these, eight were found to be significantly elevated in individuals with visceral fat accumulation and metabolic disease, defined as the presence of hyperglycemia and dyslipidemia. All of the GM3 molecular species were composed of the sphingoid base sphingosine (d18:1 (Δ4)) and, interestingly, six of the eight elevated GM3 molecular species contained a hydroxylated ceramide moiety. The hydroxylated GM3 species were, in order of decreasing abundance, d18:1-h24:0 ≈ d18:1-h24:1 > d18:1-h22:0 » d18:1-h20:0 > d18:1-h21:0 > d18:1-h18:1. Univariate and multiple linear regression analyses were conducted using a number of clinical health variables associated with obesity, type 2 diabetes, metabolic disease, atherosclerosis and hypertension. GM3(d18:1-h24:1) was identified as the best candidate for metabolic screening, proving to be significantly correlated with intima-media thickness, used for the detection of atherosclerotic disease in humans, and a number of metabolic disease risk factors including autotaxin, LDL-c and homeostatic model assessment insulin resistance (HOMA-IR).

No MeSH data available.


Related in: MedlinePlus