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Identification of Ganglioside GM3 Molecular Species in Human Serum Associated with Risk Factors of Metabolic Syndrome.

Veillon L, Go S, Matsuyama W, Suzuki A, Nagasaki M, Yatomi Y, Inokuchi J - PLoS ONE (2015)

Bottom Line: A total of 23 GM3 molecular species were measured, of these, eight were found to be significantly elevated in individuals with visceral fat accumulation and metabolic disease, defined as the presence of hyperglycemia and dyslipidemia.The hydroxylated GM3 species were, in order of decreasing abundance, d18:1-h24:0 ≈ d18:1-h24:1 > d18:1-h22:0 » d18:1-h20:0 > d18:1-h21:0 > d18:1-h18:1.Univariate and multiple linear regression analyses were conducted using a number of clinical health variables associated with obesity, type 2 diabetes, metabolic disease, atherosclerosis and hypertension.

View Article: PubMed Central - PubMed

Affiliation: Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Japan.

ABSTRACT
Serum GM3 molecular species were quantified in 125 Japanese residents using tandem mass spectrometry multiple reaction monitoring. Individuals were categorized by the presence or absence of metabolic disease risk factors including visceral fat accumulation, hyperglycemia and dyslipidemia. A total of 23 GM3 molecular species were measured, of these, eight were found to be significantly elevated in individuals with visceral fat accumulation and metabolic disease, defined as the presence of hyperglycemia and dyslipidemia. All of the GM3 molecular species were composed of the sphingoid base sphingosine (d18:1 (Δ4)) and, interestingly, six of the eight elevated GM3 molecular species contained a hydroxylated ceramide moiety. The hydroxylated GM3 species were, in order of decreasing abundance, d18:1-h24:0 ≈ d18:1-h24:1 > d18:1-h22:0 » d18:1-h20:0 > d18:1-h21:0 > d18:1-h18:1. Univariate and multiple linear regression analyses were conducted using a number of clinical health variables associated with obesity, type 2 diabetes, metabolic disease, atherosclerosis and hypertension. GM3(d18:1-h24:1) was identified as the best candidate for metabolic screening, proving to be significantly correlated with intima-media thickness, used for the detection of atherosclerotic disease in humans, and a number of metabolic disease risk factors including autotaxin, LDL-c and homeostatic model assessment insulin resistance (HOMA-IR).

No MeSH data available.


Related in: MedlinePlus

Association between metabolic disease risk factors and GM3(d18:1-h24:1).Correlation of GM3(d18:1-h24:1) with fasting blood glucose (A), insulin (B), HOMA-IR (C), HbA1c (D), total cholesterol (E), LDL-c (F), autotaxin (G) and mean IMT (H). Spearman’s rank correlation was used to access correlation between GM3(d18:1-h24:1) and metabolic disease risk factors. All correlations were deemed significant with P values below 0.05.
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pone.0129645.g003: Association between metabolic disease risk factors and GM3(d18:1-h24:1).Correlation of GM3(d18:1-h24:1) with fasting blood glucose (A), insulin (B), HOMA-IR (C), HbA1c (D), total cholesterol (E), LDL-c (F), autotaxin (G) and mean IMT (H). Spearman’s rank correlation was used to access correlation between GM3(d18:1-h24:1) and metabolic disease risk factors. All correlations were deemed significant with P values below 0.05.

Mentions: The results of multiple regression (Table 2) and univariate analyses indicated GM3(d18:1-h24:1) is significantly correlated with the largest number of metabolic disease risk factors. Spearman’s rank correlations were used to assess relationships between GM3 molecular species and clinical variables relevant to metabolic disease. Clinical variables associated with type 2 diabetes and atherosclerosis and correlated with GM3(d18:1-h24:1) include, but are not limited to, fasting blood glucose rs = 0.2633 (mg/dL, Fig 3A), insulin rs = 0.3923 (mIU/L, Fig 3B), HOMA-IR rs = 0.4226 (Fig 3C), HbA1c rs = 0.2803 (% glycated hemoglobin, Fig 3D), total cholesterol rs = 0.2045 (mg/dL, Fig 3E), LDL-c rs = 0.1899 (mg/dL, Fig 3F), autotaxin rs = 0.2934 (mg/L, Fig 3G) and mean IMT rs = 0.3709 (mm, Fig 3H).


Identification of Ganglioside GM3 Molecular Species in Human Serum Associated with Risk Factors of Metabolic Syndrome.

Veillon L, Go S, Matsuyama W, Suzuki A, Nagasaki M, Yatomi Y, Inokuchi J - PLoS ONE (2015)

Association between metabolic disease risk factors and GM3(d18:1-h24:1).Correlation of GM3(d18:1-h24:1) with fasting blood glucose (A), insulin (B), HOMA-IR (C), HbA1c (D), total cholesterol (E), LDL-c (F), autotaxin (G) and mean IMT (H). Spearman’s rank correlation was used to access correlation between GM3(d18:1-h24:1) and metabolic disease risk factors. All correlations were deemed significant with P values below 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477979&req=5

pone.0129645.g003: Association between metabolic disease risk factors and GM3(d18:1-h24:1).Correlation of GM3(d18:1-h24:1) with fasting blood glucose (A), insulin (B), HOMA-IR (C), HbA1c (D), total cholesterol (E), LDL-c (F), autotaxin (G) and mean IMT (H). Spearman’s rank correlation was used to access correlation between GM3(d18:1-h24:1) and metabolic disease risk factors. All correlations were deemed significant with P values below 0.05.
Mentions: The results of multiple regression (Table 2) and univariate analyses indicated GM3(d18:1-h24:1) is significantly correlated with the largest number of metabolic disease risk factors. Spearman’s rank correlations were used to assess relationships between GM3 molecular species and clinical variables relevant to metabolic disease. Clinical variables associated with type 2 diabetes and atherosclerosis and correlated with GM3(d18:1-h24:1) include, but are not limited to, fasting blood glucose rs = 0.2633 (mg/dL, Fig 3A), insulin rs = 0.3923 (mIU/L, Fig 3B), HOMA-IR rs = 0.4226 (Fig 3C), HbA1c rs = 0.2803 (% glycated hemoglobin, Fig 3D), total cholesterol rs = 0.2045 (mg/dL, Fig 3E), LDL-c rs = 0.1899 (mg/dL, Fig 3F), autotaxin rs = 0.2934 (mg/L, Fig 3G) and mean IMT rs = 0.3709 (mm, Fig 3H).

Bottom Line: A total of 23 GM3 molecular species were measured, of these, eight were found to be significantly elevated in individuals with visceral fat accumulation and metabolic disease, defined as the presence of hyperglycemia and dyslipidemia.The hydroxylated GM3 species were, in order of decreasing abundance, d18:1-h24:0 ≈ d18:1-h24:1 > d18:1-h22:0 » d18:1-h20:0 > d18:1-h21:0 > d18:1-h18:1.Univariate and multiple linear regression analyses were conducted using a number of clinical health variables associated with obesity, type 2 diabetes, metabolic disease, atherosclerosis and hypertension.

View Article: PubMed Central - PubMed

Affiliation: Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Japan.

ABSTRACT
Serum GM3 molecular species were quantified in 125 Japanese residents using tandem mass spectrometry multiple reaction monitoring. Individuals were categorized by the presence or absence of metabolic disease risk factors including visceral fat accumulation, hyperglycemia and dyslipidemia. A total of 23 GM3 molecular species were measured, of these, eight were found to be significantly elevated in individuals with visceral fat accumulation and metabolic disease, defined as the presence of hyperglycemia and dyslipidemia. All of the GM3 molecular species were composed of the sphingoid base sphingosine (d18:1 (Δ4)) and, interestingly, six of the eight elevated GM3 molecular species contained a hydroxylated ceramide moiety. The hydroxylated GM3 species were, in order of decreasing abundance, d18:1-h24:0 ≈ d18:1-h24:1 > d18:1-h22:0 » d18:1-h20:0 > d18:1-h21:0 > d18:1-h18:1. Univariate and multiple linear regression analyses were conducted using a number of clinical health variables associated with obesity, type 2 diabetes, metabolic disease, atherosclerosis and hypertension. GM3(d18:1-h24:1) was identified as the best candidate for metabolic screening, proving to be significantly correlated with intima-media thickness, used for the detection of atherosclerotic disease in humans, and a number of metabolic disease risk factors including autotaxin, LDL-c and homeostatic model assessment insulin resistance (HOMA-IR).

No MeSH data available.


Related in: MedlinePlus