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Vanda roxburghii chloroform extract as a potential source of polyphenols with antioxidant and cholinesterase inhibitory activities: identification of a strong phenolic antioxidant.

Uddin MN, Afrin R, Uddin MJ, Uddin MJ, Alam AH, Rahman AA, Sadik G - BMC Complement Altern Med (2015)

Bottom Line: Chloroform extract was shown to demonstrate strong ferric-reducing antioxidant power and scavenging activity against DPPH and hydroxyl free radicals when compared with the other extracts and the reference standard catechin.The antioxidant effect was further verified by inhibition of lipid peroxidation in rat brain homogenates.These results suggest that the chloroform extract of V. roxburghii, possibly due to its phenolic compounds, exert potential antioxidant and cholinesterase inhibitory activities, which may be useful in the treatment of AD.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. nasimph3@gmail.com.

ABSTRACT

Background: Alzheimer's disease (AD) is a progressively developing neurodegenerative disorder of the brain in the elderly people. Vanda roxburghii Rbr. root has been used traditionally in Bangladesh as tonic to brain and in the treatment of nervous system disorders including AD. Therefore, we aimed to investigate the cholinesterase inhibitory activities and antioxidant properties of the extracts from V. roxburghii.

Methods: The crude methanol extract from the roots of plant was sequentially fractionated with petroleum ether, chloroform, ethylacetate and water to yield their corresponding extracts. The extracts were assessed for acetylcholinesterase and butyrylcholinesterase inhibitory activity by modified Ellman method and antioxidant property by several assays including ferric reducing antioxidant power, scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and hydroxyl radical, and inhibition of lipid peroxidation. Endogenous substances in the extracts were analyzed by the standard phytochemical methods and active compound was isolated by the chromatographic methods.

Results: Chloroform extract was shown to demonstrate strong ferric-reducing antioxidant power and scavenging activity against DPPH and hydroxyl free radicals when compared with the other extracts and the reference standard catechin. The antioxidant effect was further verified by inhibition of lipid peroxidation in rat brain homogenates. Likewise, the chloroform extract exhibited the highest inhibition against both the acetylcholinesterase and butyrylcholinesterase enzymes with IC50 values of 221.13 and 82.51 μg/ml, respectively. Phytochemical screening revealed a large amount of phenolics and flavonoids in the chloroform extract. Bioactivity guided separation techniques led to the isolation of a strong antioxidant from the chloroform extract and its structure was determined as gigantol on the basis of spectral studies.

Conclusion: These results suggest that the chloroform extract of V. roxburghii, possibly due to its phenolic compounds, exert potential antioxidant and cholinesterase inhibitory activities, which may be useful in the treatment of AD.

No MeSH data available.


Related in: MedlinePlus

Percentage of lipid peroxidation inhibition at different concentration of V. roxburghii extracts and the reference standard catechin. Standard was used for comparison. Results represent mean ± SD (n = 3, (P < 0.05). PEF, petroleum ether fraction; CLF, chloroform fraction; EAF, ethylacetate fraction; AQF, aqueous fraction; CA, catechin
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Fig2: Percentage of lipid peroxidation inhibition at different concentration of V. roxburghii extracts and the reference standard catechin. Standard was used for comparison. Results represent mean ± SD (n = 3, (P < 0.05). PEF, petroleum ether fraction; CLF, chloroform fraction; EAF, ethylacetate fraction; AQF, aqueous fraction; CA, catechin

Mentions: Oxidative damage in AD predominantly manifests as lipid peroxidation and increased lipid peroxidation has been observed in the brain of AD [14, 45, 46]. Lipid peroxidation products can be measured by using thiobarbituric acid [47]. Our results (Fig. 2) showed that the chloroform extract exhibited the highest activity among the extracts tested in the inhibition of brain lipid peroxidation with an IC50 value of 27.52 μg/ml. Taken together, our results demonstrated the potential antioxidant activity of the chloroform extract of V. roxburghii.Fig. 2


Vanda roxburghii chloroform extract as a potential source of polyphenols with antioxidant and cholinesterase inhibitory activities: identification of a strong phenolic antioxidant.

Uddin MN, Afrin R, Uddin MJ, Uddin MJ, Alam AH, Rahman AA, Sadik G - BMC Complement Altern Med (2015)

Percentage of lipid peroxidation inhibition at different concentration of V. roxburghii extracts and the reference standard catechin. Standard was used for comparison. Results represent mean ± SD (n = 3, (P < 0.05). PEF, petroleum ether fraction; CLF, chloroform fraction; EAF, ethylacetate fraction; AQF, aqueous fraction; CA, catechin
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4477535&req=5

Fig2: Percentage of lipid peroxidation inhibition at different concentration of V. roxburghii extracts and the reference standard catechin. Standard was used for comparison. Results represent mean ± SD (n = 3, (P < 0.05). PEF, petroleum ether fraction; CLF, chloroform fraction; EAF, ethylacetate fraction; AQF, aqueous fraction; CA, catechin
Mentions: Oxidative damage in AD predominantly manifests as lipid peroxidation and increased lipid peroxidation has been observed in the brain of AD [14, 45, 46]. Lipid peroxidation products can be measured by using thiobarbituric acid [47]. Our results (Fig. 2) showed that the chloroform extract exhibited the highest activity among the extracts tested in the inhibition of brain lipid peroxidation with an IC50 value of 27.52 μg/ml. Taken together, our results demonstrated the potential antioxidant activity of the chloroform extract of V. roxburghii.Fig. 2

Bottom Line: Chloroform extract was shown to demonstrate strong ferric-reducing antioxidant power and scavenging activity against DPPH and hydroxyl free radicals when compared with the other extracts and the reference standard catechin.The antioxidant effect was further verified by inhibition of lipid peroxidation in rat brain homogenates.These results suggest that the chloroform extract of V. roxburghii, possibly due to its phenolic compounds, exert potential antioxidant and cholinesterase inhibitory activities, which may be useful in the treatment of AD.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. nasimph3@gmail.com.

ABSTRACT

Background: Alzheimer's disease (AD) is a progressively developing neurodegenerative disorder of the brain in the elderly people. Vanda roxburghii Rbr. root has been used traditionally in Bangladesh as tonic to brain and in the treatment of nervous system disorders including AD. Therefore, we aimed to investigate the cholinesterase inhibitory activities and antioxidant properties of the extracts from V. roxburghii.

Methods: The crude methanol extract from the roots of plant was sequentially fractionated with petroleum ether, chloroform, ethylacetate and water to yield their corresponding extracts. The extracts were assessed for acetylcholinesterase and butyrylcholinesterase inhibitory activity by modified Ellman method and antioxidant property by several assays including ferric reducing antioxidant power, scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and hydroxyl radical, and inhibition of lipid peroxidation. Endogenous substances in the extracts were analyzed by the standard phytochemical methods and active compound was isolated by the chromatographic methods.

Results: Chloroform extract was shown to demonstrate strong ferric-reducing antioxidant power and scavenging activity against DPPH and hydroxyl free radicals when compared with the other extracts and the reference standard catechin. The antioxidant effect was further verified by inhibition of lipid peroxidation in rat brain homogenates. Likewise, the chloroform extract exhibited the highest inhibition against both the acetylcholinesterase and butyrylcholinesterase enzymes with IC50 values of 221.13 and 82.51 μg/ml, respectively. Phytochemical screening revealed a large amount of phenolics and flavonoids in the chloroform extract. Bioactivity guided separation techniques led to the isolation of a strong antioxidant from the chloroform extract and its structure was determined as gigantol on the basis of spectral studies.

Conclusion: These results suggest that the chloroform extract of V. roxburghii, possibly due to its phenolic compounds, exert potential antioxidant and cholinesterase inhibitory activities, which may be useful in the treatment of AD.

No MeSH data available.


Related in: MedlinePlus