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The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus).

Wang X, Liu Q, Xiao Y, Yang Y, Wang Y, Song Z, You F, An H, Xiao Z, Xu S, Ma D, Li J - Biomed Res Int (2015)

Bottom Line: The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable.The Podnd transcripts localization and migration were similar to zebrafish.Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

View Article: PubMed Central - PubMed

Affiliation: Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd. The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable. The Podnd transcripts localization and migration were similar to zebrafish. Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

No MeSH data available.


Related in: MedlinePlus

Schematic summary of Podnd transcripts distribution during embryogenesis in olive flounder. Red spots indicate Podnd signals. (a) 2-cell, (b) 4-cell, (c) 8-cell, and (d) 16-cell stages. ((a)–(d)) Podnd are located at the end of the first, second, and third cleavage furrows, (e) 32-cell stage: the signals aggregated into four single blastomeres, (f) morula stage, (g) blastula stage: the Podnd-positive cells increased from one to two and the sister cells are located adjacent to each other at every positive area, (h) early gastrula: Podnd mRNA mainly localized at the marginal region of the blastoderm, (i) mid gastrula stage: some Podnd-positive cells are located at the germ ring and the others moved towards to the dorsal side, ((g1) and (g2)) late gastrula stage, (g1) side view and (g2) top view: Podnd-positive cells aligned along both sides of the body axes in the posterior of the trunk region, (k) somite stage: Podnd-positive cells aligned as two lines along both sides of the embryonic body in the trunk region, (l) the Podnd-positive cells formed clusters above the hind gut in the presumptive genital ridge, (m) 1-2 d after hatching: Podnd-positive cells cluster in the presumptive genital ridge region at the posterior dorsal sides of gut, and (n) cross section: dnd-positive cells aligned at the genital ridge which was at the dorsal sides of the hind gut and ventral side of notochord.
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fig5: Schematic summary of Podnd transcripts distribution during embryogenesis in olive flounder. Red spots indicate Podnd signals. (a) 2-cell, (b) 4-cell, (c) 8-cell, and (d) 16-cell stages. ((a)–(d)) Podnd are located at the end of the first, second, and third cleavage furrows, (e) 32-cell stage: the signals aggregated into four single blastomeres, (f) morula stage, (g) blastula stage: the Podnd-positive cells increased from one to two and the sister cells are located adjacent to each other at every positive area, (h) early gastrula: Podnd mRNA mainly localized at the marginal region of the blastoderm, (i) mid gastrula stage: some Podnd-positive cells are located at the germ ring and the others moved towards to the dorsal side, ((g1) and (g2)) late gastrula stage, (g1) side view and (g2) top view: Podnd-positive cells aligned along both sides of the body axes in the posterior of the trunk region, (k) somite stage: Podnd-positive cells aligned as two lines along both sides of the embryonic body in the trunk region, (l) the Podnd-positive cells formed clusters above the hind gut in the presumptive genital ridge, (m) 1-2 d after hatching: Podnd-positive cells cluster in the presumptive genital ridge region at the posterior dorsal sides of gut, and (n) cross section: dnd-positive cells aligned at the genital ridge which was at the dorsal sides of the hind gut and ventral side of notochord.

Mentions: The localization of Podnd transcripts was investigated by WISH during embryogenesis from 2-cell stage to 2 days after hatching to reveal the origin and migration of PGCs. At the 2-cell stage (Figures 4(a) and 5(a)), Podnd mRNA was detected at either end of the first cleavage furrow. At the 4-cell (Figures 4(b) and 5(b)) and 8-cell (Figures 4(c) and 5(c)) stages, mRNA aggregations had split into four clusters which remained located at the end of the first and second cleavage furrows. As the embryo developed to 32-cell stage (Figures 4(e) and 5(e)), the signals aggregated into four single blastomeres and formed presumptive PGCs. At morula stage (Figures 4(f) and 5(f)), the four Podnd-positive cells were still located at the margin of blastoderm. During the blastula stage (Figures 4(g1), 4(g2), and 5(g)), the Podnd-positive cells increased from one to two and the sister cells were located adjacent to each other at every positive area and then formed PGCs.


The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus).

Wang X, Liu Q, Xiao Y, Yang Y, Wang Y, Song Z, You F, An H, Xiao Z, Xu S, Ma D, Li J - Biomed Res Int (2015)

Schematic summary of Podnd transcripts distribution during embryogenesis in olive flounder. Red spots indicate Podnd signals. (a) 2-cell, (b) 4-cell, (c) 8-cell, and (d) 16-cell stages. ((a)–(d)) Podnd are located at the end of the first, second, and third cleavage furrows, (e) 32-cell stage: the signals aggregated into four single blastomeres, (f) morula stage, (g) blastula stage: the Podnd-positive cells increased from one to two and the sister cells are located adjacent to each other at every positive area, (h) early gastrula: Podnd mRNA mainly localized at the marginal region of the blastoderm, (i) mid gastrula stage: some Podnd-positive cells are located at the germ ring and the others moved towards to the dorsal side, ((g1) and (g2)) late gastrula stage, (g1) side view and (g2) top view: Podnd-positive cells aligned along both sides of the body axes in the posterior of the trunk region, (k) somite stage: Podnd-positive cells aligned as two lines along both sides of the embryonic body in the trunk region, (l) the Podnd-positive cells formed clusters above the hind gut in the presumptive genital ridge, (m) 1-2 d after hatching: Podnd-positive cells cluster in the presumptive genital ridge region at the posterior dorsal sides of gut, and (n) cross section: dnd-positive cells aligned at the genital ridge which was at the dorsal sides of the hind gut and ventral side of notochord.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig5: Schematic summary of Podnd transcripts distribution during embryogenesis in olive flounder. Red spots indicate Podnd signals. (a) 2-cell, (b) 4-cell, (c) 8-cell, and (d) 16-cell stages. ((a)–(d)) Podnd are located at the end of the first, second, and third cleavage furrows, (e) 32-cell stage: the signals aggregated into four single blastomeres, (f) morula stage, (g) blastula stage: the Podnd-positive cells increased from one to two and the sister cells are located adjacent to each other at every positive area, (h) early gastrula: Podnd mRNA mainly localized at the marginal region of the blastoderm, (i) mid gastrula stage: some Podnd-positive cells are located at the germ ring and the others moved towards to the dorsal side, ((g1) and (g2)) late gastrula stage, (g1) side view and (g2) top view: Podnd-positive cells aligned along both sides of the body axes in the posterior of the trunk region, (k) somite stage: Podnd-positive cells aligned as two lines along both sides of the embryonic body in the trunk region, (l) the Podnd-positive cells formed clusters above the hind gut in the presumptive genital ridge, (m) 1-2 d after hatching: Podnd-positive cells cluster in the presumptive genital ridge region at the posterior dorsal sides of gut, and (n) cross section: dnd-positive cells aligned at the genital ridge which was at the dorsal sides of the hind gut and ventral side of notochord.
Mentions: The localization of Podnd transcripts was investigated by WISH during embryogenesis from 2-cell stage to 2 days after hatching to reveal the origin and migration of PGCs. At the 2-cell stage (Figures 4(a) and 5(a)), Podnd mRNA was detected at either end of the first cleavage furrow. At the 4-cell (Figures 4(b) and 5(b)) and 8-cell (Figures 4(c) and 5(c)) stages, mRNA aggregations had split into four clusters which remained located at the end of the first and second cleavage furrows. As the embryo developed to 32-cell stage (Figures 4(e) and 5(e)), the signals aggregated into four single blastomeres and formed presumptive PGCs. At morula stage (Figures 4(f) and 5(f)), the four Podnd-positive cells were still located at the margin of blastoderm. During the blastula stage (Figures 4(g1), 4(g2), and 5(g)), the Podnd-positive cells increased from one to two and the sister cells were located adjacent to each other at every positive area and then formed PGCs.

Bottom Line: The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable.The Podnd transcripts localization and migration were similar to zebrafish.Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

View Article: PubMed Central - PubMed

Affiliation: Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd. The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable. The Podnd transcripts localization and migration were similar to zebrafish. Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

No MeSH data available.


Related in: MedlinePlus