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The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus).

Wang X, Liu Q, Xiao Y, Yang Y, Wang Y, Song Z, You F, An H, Xiao Z, Xu S, Ma D, Li J - Biomed Res Int (2015)

Bottom Line: The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable.The Podnd transcripts localization and migration were similar to zebrafish.Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

View Article: PubMed Central - PubMed

Affiliation: Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd. The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable. The Podnd transcripts localization and migration were similar to zebrafish. Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

No MeSH data available.


Related in: MedlinePlus

Distribution of Podnd transcripts in gonad germ cells by SISH. (a) Paraffin sections of ovary were stained by HE; (b) paraffin sections of ovary were hybridized with Podnd antisense probe. On ovarian sections, the strongest signal was present in certain oogonia and oocytes at stage II (diffused in the ooplasm), whereas, difficult to discern in oocytes at stage V, Podnd transcripts were diffused throughout the ooplasm. (c) Paraffin sections of testis were stained by HE; (d) paraffin sections of testis were hybridized with Podnd antisense probe. Podnd signal was strong in spermatogonia, weak in spermatocytes, and absent in spermatids. Og: oogonia; I-II: previtellogenic oocytes; III: early vitellogenic oocytes; IV: midvitellogenic oocytes; V: late vitellogenic oocytes; Sg: spermatogonia; Sc: spermatocytes; St: spermatids. Scale bar: 100 µm ((a)-(b)), 50 μm ((c)-(d)).
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fig3: Distribution of Podnd transcripts in gonad germ cells by SISH. (a) Paraffin sections of ovary were stained by HE; (b) paraffin sections of ovary were hybridized with Podnd antisense probe. On ovarian sections, the strongest signal was present in certain oogonia and oocytes at stage II (diffused in the ooplasm), whereas, difficult to discern in oocytes at stage V, Podnd transcripts were diffused throughout the ooplasm. (c) Paraffin sections of testis were stained by HE; (d) paraffin sections of testis were hybridized with Podnd antisense probe. Podnd signal was strong in spermatogonia, weak in spermatocytes, and absent in spermatids. Og: oogonia; I-II: previtellogenic oocytes; III: early vitellogenic oocytes; IV: midvitellogenic oocytes; V: late vitellogenic oocytes; Sg: spermatogonia; Sc: spermatocytes; St: spermatids. Scale bar: 100 µm ((a)-(b)), 50 μm ((c)-(d)).

Mentions: Tissue section in situ hybridization was carried out to investigate the Podnd expression pattern of adult gonad. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. In ovarian sections, the strongest signal was present in certain oogonia and oocytes at stage II (diffused in the ooplasm) whereas it was difficult to discern in oocytes at stage V (Figure 3(b)). In testis, obvious signal was present in spermatogonium, weaker in spermatocyte, and undetectable in spermatid and spermatozoon (Figure 3(d)).


The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus).

Wang X, Liu Q, Xiao Y, Yang Y, Wang Y, Song Z, You F, An H, Xiao Z, Xu S, Ma D, Li J - Biomed Res Int (2015)

Distribution of Podnd transcripts in gonad germ cells by SISH. (a) Paraffin sections of ovary were stained by HE; (b) paraffin sections of ovary were hybridized with Podnd antisense probe. On ovarian sections, the strongest signal was present in certain oogonia and oocytes at stage II (diffused in the ooplasm), whereas, difficult to discern in oocytes at stage V, Podnd transcripts were diffused throughout the ooplasm. (c) Paraffin sections of testis were stained by HE; (d) paraffin sections of testis were hybridized with Podnd antisense probe. Podnd signal was strong in spermatogonia, weak in spermatocytes, and absent in spermatids. Og: oogonia; I-II: previtellogenic oocytes; III: early vitellogenic oocytes; IV: midvitellogenic oocytes; V: late vitellogenic oocytes; Sg: spermatogonia; Sc: spermatocytes; St: spermatids. Scale bar: 100 µm ((a)-(b)), 50 μm ((c)-(d)).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4477439&req=5

fig3: Distribution of Podnd transcripts in gonad germ cells by SISH. (a) Paraffin sections of ovary were stained by HE; (b) paraffin sections of ovary were hybridized with Podnd antisense probe. On ovarian sections, the strongest signal was present in certain oogonia and oocytes at stage II (diffused in the ooplasm), whereas, difficult to discern in oocytes at stage V, Podnd transcripts were diffused throughout the ooplasm. (c) Paraffin sections of testis were stained by HE; (d) paraffin sections of testis were hybridized with Podnd antisense probe. Podnd signal was strong in spermatogonia, weak in spermatocytes, and absent in spermatids. Og: oogonia; I-II: previtellogenic oocytes; III: early vitellogenic oocytes; IV: midvitellogenic oocytes; V: late vitellogenic oocytes; Sg: spermatogonia; Sc: spermatocytes; St: spermatids. Scale bar: 100 µm ((a)-(b)), 50 μm ((c)-(d)).
Mentions: Tissue section in situ hybridization was carried out to investigate the Podnd expression pattern of adult gonad. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. In ovarian sections, the strongest signal was present in certain oogonia and oocytes at stage II (diffused in the ooplasm) whereas it was difficult to discern in oocytes at stage V (Figure 3(b)). In testis, obvious signal was present in spermatogonium, weaker in spermatocyte, and undetectable in spermatid and spermatozoon (Figure 3(d)).

Bottom Line: The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable.The Podnd transcripts localization and migration were similar to zebrafish.Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

View Article: PubMed Central - PubMed

Affiliation: Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd. The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable. The Podnd transcripts localization and migration were similar to zebrafish. Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

No MeSH data available.


Related in: MedlinePlus