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The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus).

Wang X, Liu Q, Xiao Y, Yang Y, Wang Y, Song Z, You F, An H, Xiao Z, Xu S, Ma D, Li J - Biomed Res Int (2015)

Bottom Line: The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable.The Podnd transcripts localization and migration were similar to zebrafish.Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

View Article: PubMed Central - PubMed

Affiliation: Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd. The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable. The Podnd transcripts localization and migration were similar to zebrafish. Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

No MeSH data available.


Related in: MedlinePlus

Expression of Podnd mRNA determined by qRT-PCR. (a) Tissue-specific expression of Podnd mRNA determined by qRT-PCR. (b) Embryonic expression of Podnd determined by qRT-PCR.
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fig2: Expression of Podnd mRNA determined by qRT-PCR. (a) Tissue-specific expression of Podnd mRNA determined by qRT-PCR. (b) Embryonic expression of Podnd determined by qRT-PCR.

Mentions: Podnd mRNA expression levels in different tissues including heart, brain, liver, intestine, kidney, spleen, muscle, gill, ovary, and testis of mature olive flounder by qRT-PCR were performed. Total RNA was extracted from tissues of healthy adults, each sample of different developmental stages during embryogenesis, and reversely transcribed. The results showed that tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected (Figure 2(a)). In addition, the expression of Podnd transcripts was much higher in the ovary compared with that in the testis.


The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus).

Wang X, Liu Q, Xiao Y, Yang Y, Wang Y, Song Z, You F, An H, Xiao Z, Xu S, Ma D, Li J - Biomed Res Int (2015)

Expression of Podnd mRNA determined by qRT-PCR. (a) Tissue-specific expression of Podnd mRNA determined by qRT-PCR. (b) Embryonic expression of Podnd determined by qRT-PCR.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477439&req=5

fig2: Expression of Podnd mRNA determined by qRT-PCR. (a) Tissue-specific expression of Podnd mRNA determined by qRT-PCR. (b) Embryonic expression of Podnd determined by qRT-PCR.
Mentions: Podnd mRNA expression levels in different tissues including heart, brain, liver, intestine, kidney, spleen, muscle, gill, ovary, and testis of mature olive flounder by qRT-PCR were performed. Total RNA was extracted from tissues of healthy adults, each sample of different developmental stages during embryogenesis, and reversely transcribed. The results showed that tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected (Figure 2(a)). In addition, the expression of Podnd transcripts was much higher in the ovary compared with that in the testis.

Bottom Line: The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable.The Podnd transcripts localization and migration were similar to zebrafish.Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

View Article: PubMed Central - PubMed

Affiliation: Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd. The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable. The Podnd transcripts localization and migration were similar to zebrafish. Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

No MeSH data available.


Related in: MedlinePlus