Limits...
The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus).

Wang X, Liu Q, Xiao Y, Yang Y, Wang Y, Song Z, You F, An H, Xiao Z, Xu S, Ma D, Li J - Biomed Res Int (2015)

Bottom Line: The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable.The Podnd transcripts localization and migration were similar to zebrafish.Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

View Article: PubMed Central - PubMed

Affiliation: Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd. The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable. The Podnd transcripts localization and migration were similar to zebrafish. Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

No MeSH data available.


Related in: MedlinePlus

Comparison of DND proteins. (a) Multiple alignment was carried out using AlignX program. The conserved regions are indicated in frame. NR: N-terminal region; RRM: RNA recognition motif; CR1–4: C-terminal regions. (b) Phylogenetic tree of DND proteins. It was established by MEGA6 with neighbor-joining method. The numbers under the node indicate the bootstrap value as percentages obtained for 1000 replicates. (c) Comparison of the identities of DND homologues between olive flounder and other organisms.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4477439&req=5

fig1: Comparison of DND proteins. (a) Multiple alignment was carried out using AlignX program. The conserved regions are indicated in frame. NR: N-terminal region; RRM: RNA recognition motif; CR1–4: C-terminal regions. (b) Phylogenetic tree of DND proteins. It was established by MEGA6 with neighbor-joining method. The numbers under the node indicate the bootstrap value as percentages obtained for 1000 replicates. (c) Comparison of the identities of DND homologues between olive flounder and other organisms.

Mentions: A partial cDNA fragment (176 bp) of dnd was isolated from Paralichthys olivaceus ovary. The full sequence of Podnd was obtained successfully by using 3′- and 5′-RACE. The full length of Podnd cDNA is 1446 bp, containing a 1178 bp open reading frame. It encodes a putative protein of 392 amino acids, an 18 bp 5′-untranslated region (UTR), and a 249 bp 3′-UTR. The nucleotide sequence of Podnd had been submitted to GenBank (accession number: KP224455). The deduced amino acid sequences were aligned using the AlignX program in Vector NTI suite 11.5 software package (Figure 1(a)).


The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus).

Wang X, Liu Q, Xiao Y, Yang Y, Wang Y, Song Z, You F, An H, Xiao Z, Xu S, Ma D, Li J - Biomed Res Int (2015)

Comparison of DND proteins. (a) Multiple alignment was carried out using AlignX program. The conserved regions are indicated in frame. NR: N-terminal region; RRM: RNA recognition motif; CR1–4: C-terminal regions. (b) Phylogenetic tree of DND proteins. It was established by MEGA6 with neighbor-joining method. The numbers under the node indicate the bootstrap value as percentages obtained for 1000 replicates. (c) Comparison of the identities of DND homologues between olive flounder and other organisms.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477439&req=5

fig1: Comparison of DND proteins. (a) Multiple alignment was carried out using AlignX program. The conserved regions are indicated in frame. NR: N-terminal region; RRM: RNA recognition motif; CR1–4: C-terminal regions. (b) Phylogenetic tree of DND proteins. It was established by MEGA6 with neighbor-joining method. The numbers under the node indicate the bootstrap value as percentages obtained for 1000 replicates. (c) Comparison of the identities of DND homologues between olive flounder and other organisms.
Mentions: A partial cDNA fragment (176 bp) of dnd was isolated from Paralichthys olivaceus ovary. The full sequence of Podnd was obtained successfully by using 3′- and 5′-RACE. The full length of Podnd cDNA is 1446 bp, containing a 1178 bp open reading frame. It encodes a putative protein of 392 amino acids, an 18 bp 5′-untranslated region (UTR), and a 249 bp 3′-UTR. The nucleotide sequence of Podnd had been submitted to GenBank (accession number: KP224455). The deduced amino acid sequences were aligned using the AlignX program in Vector NTI suite 11.5 software package (Figure 1(a)).

Bottom Line: The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable.The Podnd transcripts localization and migration were similar to zebrafish.Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

View Article: PubMed Central - PubMed

Affiliation: Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd. The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable. The Podnd transcripts localization and migration were similar to zebrafish. Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.

No MeSH data available.


Related in: MedlinePlus