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Beninese Medicinal Plants as a Source of Antimycobacterial Agents: Bioguided Fractionation and In Vitro Activity of Alkaloids Isolated from Holarrhena floribunda Used in Traditional Treatment of Buruli Ulcer.

Yemoa A, Gbenou J, Affolabi D, Moudachirou M, Bigot A, Anagonou S, Portaels F, Martin A, Quetin-Leclercq J - Biomed Res Int (2015)

Bottom Line: Research is needed to find cost-effective therapies for this costly disease.Durand and Schinz was found to have significant antimycobacterial activity against M. ulcerans (MIC = 125 µg/mL).These results give some support to the use of this plant in traditional medicine.

View Article: PubMed Central - PubMed

Affiliation: Unité de Formation et de Recherche en Pharmacie, Faculté des Sciences de la Santé (FSS), Université d'Abomey Calavi (UAC), 04 BP 494 Cotonou, Benin.

ABSTRACT
Buruli ulcer (BU) imposes a serious economic burden on affected households and on health systems that are involved in diagnosing the disease and treating patients. Research is needed to find cost-effective therapies for this costly disease. Plants have always been an important source of new pharmacologically active molecules. Consequently we decided to undertake the study of plants used in traditional treatment of BU in Benin and investigate their antimycobacterial activity as well as their chemical composition. Extracts from forty-four (44) plant species were selected on account of reported traditional uses for the treatment of BU in Benin and were assayed for antimycobacterial activities. Crude hydroethanolic extract from aerial parts of Holarrhena floribunda (G. Don) T. Durand and Schinz was found to have significant antimycobacterial activity against M. ulcerans (MIC = 125 µg/mL). We describe here the identification of four steroidal alkaloids from Mycobacterium ulcerans growth-inhibiting fractions of the alkaloidal extract of the aerial parts of Holarrhena floribunda. Holadysamine was purified in sufficient amount to allow the determination of its MCI (=50 µg/mL). These results give some support to the use of this plant in traditional medicine.

No MeSH data available.


Related in: MedlinePlus

Scheme for preparation of alkaloids enriched extract.
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Related In: Results  -  Collection


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fig2: Scheme for preparation of alkaloids enriched extract.

Mentions: Crude hydroethanolic extract was fractionated on silica gel 60 (0,063–0,200 mm Merck, Germany) by atmospheric pressure liquid chromatography eluting with solvents of increasing polarity, namely, hexane, dichloromethane, ethyl acetate, and water, yielding 4 fractions (F1, F2, F3, and F4). Of these, fraction F2 (dichloromethane) was found to cause growth inhibition of M. ulcerans and was, as a result, selected for further work. This fraction was monitored using TLC plates (TLC silica gel 60F254S, Merck) and led to characterize the presence of alkaloids (toluene : ethyl acetate : diethylamine: [7 : 2 : 1], detection-Dragendorff's spray reagent). We then performed a more specific extraction to obtain an enriched alkaloid extract (Figure 2).


Beninese Medicinal Plants as a Source of Antimycobacterial Agents: Bioguided Fractionation and In Vitro Activity of Alkaloids Isolated from Holarrhena floribunda Used in Traditional Treatment of Buruli Ulcer.

Yemoa A, Gbenou J, Affolabi D, Moudachirou M, Bigot A, Anagonou S, Portaels F, Martin A, Quetin-Leclercq J - Biomed Res Int (2015)

Scheme for preparation of alkaloids enriched extract.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477427&req=5

fig2: Scheme for preparation of alkaloids enriched extract.
Mentions: Crude hydroethanolic extract was fractionated on silica gel 60 (0,063–0,200 mm Merck, Germany) by atmospheric pressure liquid chromatography eluting with solvents of increasing polarity, namely, hexane, dichloromethane, ethyl acetate, and water, yielding 4 fractions (F1, F2, F3, and F4). Of these, fraction F2 (dichloromethane) was found to cause growth inhibition of M. ulcerans and was, as a result, selected for further work. This fraction was monitored using TLC plates (TLC silica gel 60F254S, Merck) and led to characterize the presence of alkaloids (toluene : ethyl acetate : diethylamine: [7 : 2 : 1], detection-Dragendorff's spray reagent). We then performed a more specific extraction to obtain an enriched alkaloid extract (Figure 2).

Bottom Line: Research is needed to find cost-effective therapies for this costly disease.Durand and Schinz was found to have significant antimycobacterial activity against M. ulcerans (MIC = 125 µg/mL).These results give some support to the use of this plant in traditional medicine.

View Article: PubMed Central - PubMed

Affiliation: Unité de Formation et de Recherche en Pharmacie, Faculté des Sciences de la Santé (FSS), Université d'Abomey Calavi (UAC), 04 BP 494 Cotonou, Benin.

ABSTRACT
Buruli ulcer (BU) imposes a serious economic burden on affected households and on health systems that are involved in diagnosing the disease and treating patients. Research is needed to find cost-effective therapies for this costly disease. Plants have always been an important source of new pharmacologically active molecules. Consequently we decided to undertake the study of plants used in traditional treatment of BU in Benin and investigate their antimycobacterial activity as well as their chemical composition. Extracts from forty-four (44) plant species were selected on account of reported traditional uses for the treatment of BU in Benin and were assayed for antimycobacterial activities. Crude hydroethanolic extract from aerial parts of Holarrhena floribunda (G. Don) T. Durand and Schinz was found to have significant antimycobacterial activity against M. ulcerans (MIC = 125 µg/mL). We describe here the identification of four steroidal alkaloids from Mycobacterium ulcerans growth-inhibiting fractions of the alkaloidal extract of the aerial parts of Holarrhena floribunda. Holadysamine was purified in sufficient amount to allow the determination of its MCI (=50 µg/mL). These results give some support to the use of this plant in traditional medicine.

No MeSH data available.


Related in: MedlinePlus