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Isolation and full-length genome analysis of mosquito-borne Manzanilla virus from Yunnan Province, China.

Feng Y, Fu SH, Yang WH, Zhang YZ, He B, Tu CC, Liang GD, Zhang HL - BMC Res Notes (2015)

Bottom Line: Nucleotide homology ranged between 96 and 99% between DHL10M107 and VN04-2108.DHL10M107 is likely a MANV isolated from mosquitos in the Yunnan Province.This is the first reported isolation of MANV in mainland China.

View Article: PubMed Central - PubMed

Affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, 671000, People's Republic of China. ynfy428@163.com.

ABSTRACT

Background: There have been four strains on Manzanilla virus (MANV) identified to date. Here, we identify a novel MANV strain (DHL10M107) isolated from Culex tritaeniorhynchus Giles mosquitoes from Ruili city, Dehong prefecture, Yunnan Province, in the People's Republic of China.

Results: The DHL10M107 L, M and S genes were sequenced at the nucleotide and deduced amino acid levels. The L, M and S gene sequences of DHL10M107 clustered with the MANV strains VN04-2108, TRVL3587, SA An 4165, and AV 782. DHL10M107 was most closely related to VN04-2108. Nucleotide homology ranged between 96 and 99% between DHL10M107 and VN04-2108. In terms of amino acid homology, all of the amino acid differences were in the L (96.3% homologous) and M (97.7% homologous) fragments.

Conclusions: DHL10M107 is likely a MANV isolated from mosquitos in the Yunnan Province. This is the first reported isolation of MANV in mainland China.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic trees based on the nucleotide sequences of DHL10M107 strains isolated from Yunnan Province, China. a L sequence, b M sequence, c S sequence and d partial Oya virus S sequence (365 bp). The black triangle indicates DHL10M107.
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Fig1: Phylogenetic trees based on the nucleotide sequences of DHL10M107 strains isolated from Yunnan Province, China. a L sequence, b M sequence, c S sequence and d partial Oya virus S sequence (365 bp). The black triangle indicates DHL10M107.

Mentions: Phylogenetic analysis of the viral L and M gene sequences indicated that DHL10M107 clustered with MANV (VN04-2108) and an Oya virus (OYV) strain (SC0806) isolated from mosquitos in Sichuan province, China [17], although it was more closely related to VN04-2108 (Figure 1a, b). However, in terms of the S fragments, while DHL10M107 was in the same clade as VN04-2108 and SC0806, it formed a secondary clade, and the S fragments of VN04-2108 and SC0806 were more closely related (Figure 1c). Regardless of the genome fragment, DHL10M107 was closely related to three MANV strains (TRVL3587, SA An 4165, and AV 782; Figure 1). The OYV reported by Kono et al. [18] had a partial sequence for the S fragment in GenBank (accession no. AB075611). Similar to Figure 1c, the phylogenetic tree constructed using this sequence indicated AB075611, DHL10M107, VN04-2108, and SC0806 were in the same clade and had similar evolutionary relationships (Figure 1d).Figure 1


Isolation and full-length genome analysis of mosquito-borne Manzanilla virus from Yunnan Province, China.

Feng Y, Fu SH, Yang WH, Zhang YZ, He B, Tu CC, Liang GD, Zhang HL - BMC Res Notes (2015)

Phylogenetic trees based on the nucleotide sequences of DHL10M107 strains isolated from Yunnan Province, China. a L sequence, b M sequence, c S sequence and d partial Oya virus S sequence (365 bp). The black triangle indicates DHL10M107.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4477421&req=5

Fig1: Phylogenetic trees based on the nucleotide sequences of DHL10M107 strains isolated from Yunnan Province, China. a L sequence, b M sequence, c S sequence and d partial Oya virus S sequence (365 bp). The black triangle indicates DHL10M107.
Mentions: Phylogenetic analysis of the viral L and M gene sequences indicated that DHL10M107 clustered with MANV (VN04-2108) and an Oya virus (OYV) strain (SC0806) isolated from mosquitos in Sichuan province, China [17], although it was more closely related to VN04-2108 (Figure 1a, b). However, in terms of the S fragments, while DHL10M107 was in the same clade as VN04-2108 and SC0806, it formed a secondary clade, and the S fragments of VN04-2108 and SC0806 were more closely related (Figure 1c). Regardless of the genome fragment, DHL10M107 was closely related to three MANV strains (TRVL3587, SA An 4165, and AV 782; Figure 1). The OYV reported by Kono et al. [18] had a partial sequence for the S fragment in GenBank (accession no. AB075611). Similar to Figure 1c, the phylogenetic tree constructed using this sequence indicated AB075611, DHL10M107, VN04-2108, and SC0806 were in the same clade and had similar evolutionary relationships (Figure 1d).Figure 1

Bottom Line: Nucleotide homology ranged between 96 and 99% between DHL10M107 and VN04-2108.DHL10M107 is likely a MANV isolated from mosquitos in the Yunnan Province.This is the first reported isolation of MANV in mainland China.

View Article: PubMed Central - PubMed

Affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, 671000, People's Republic of China. ynfy428@163.com.

ABSTRACT

Background: There have been four strains on Manzanilla virus (MANV) identified to date. Here, we identify a novel MANV strain (DHL10M107) isolated from Culex tritaeniorhynchus Giles mosquitoes from Ruili city, Dehong prefecture, Yunnan Province, in the People's Republic of China.

Results: The DHL10M107 L, M and S genes were sequenced at the nucleotide and deduced amino acid levels. The L, M and S gene sequences of DHL10M107 clustered with the MANV strains VN04-2108, TRVL3587, SA An 4165, and AV 782. DHL10M107 was most closely related to VN04-2108. Nucleotide homology ranged between 96 and 99% between DHL10M107 and VN04-2108. In terms of amino acid homology, all of the amino acid differences were in the L (96.3% homologous) and M (97.7% homologous) fragments.

Conclusions: DHL10M107 is likely a MANV isolated from mosquitos in the Yunnan Province. This is the first reported isolation of MANV in mainland China.

No MeSH data available.


Related in: MedlinePlus