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Latent progenitor cells as potential regulators for tympanic membrane regeneration.

Kim SW, Kim J, Seonwoo H, Jang KJ, Kim YJ, Lim HJ, Lim KT, Tian C, Chung JH, Choung YH - Sci Rep (2015)

Bottom Line: Here, we explored an approach for TM regeneration where the latent progenitor or stem cells within TM epithelial layers may play an important regulatory role.Additionally, they are present at high levels in perforated TMs, especially in proximity to the holes, regardless of acute or chronic status, suggesting that TM stem cells may be a potential factor for TM regeneration.Our study suggests that latent TM stem cells could be potential regulators of regeneration, which provides a new insight into this clinically important process and a potential target for new therapies for chronic otitis media and other eardrum injuries.

View Article: PubMed Central - PubMed

Affiliation: Department of Burns and Plastic Surgery, Affiliated Hospital of Yanbian University, 1327 Juzi Street, Yanji, Jilin 133000, P.R. China.

ABSTRACT
Tympanic membrane (TM) perforation, in particular chronic otitis media, is one of the most common clinical problems in the world and can present with sensorineural healing loss. Here, we explored an approach for TM regeneration where the latent progenitor or stem cells within TM epithelial layers may play an important regulatory role. We showed that potential TM stem cells present highly positive staining for epithelial stem cell markers in all areas of normal TM tissue. Additionally, they are present at high levels in perforated TMs, especially in proximity to the holes, regardless of acute or chronic status, suggesting that TM stem cells may be a potential factor for TM regeneration. Our study suggests that latent TM stem cells could be potential regulators of regeneration, which provides a new insight into this clinically important process and a potential target for new therapies for chronic otitis media and other eardrum injuries.

No MeSH data available.


Related in: MedlinePlus

The intensity of expression on potential stem cell makers (Integrin β1 (red color) and Cytokeratin 19 (blue color) according to the anatomic areas (malleus handle; annulus (perforation site); pars tensa; annulus (unperforation site) in the TMs with acute or chronic perforations.a) Acute TM perforation model. (b) Chronic TM perforation model.
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f5: The intensity of expression on potential stem cell makers (Integrin β1 (red color) and Cytokeratin 19 (blue color) according to the anatomic areas (malleus handle; annulus (perforation site); pars tensa; annulus (unperforation site) in the TMs with acute or chronic perforations.a) Acute TM perforation model. (b) Chronic TM perforation model.

Mentions: We quantitatively analyzed and compared the expression intensity of INGβ1 and CK19 in landmarked locations in the TM at different time points (Fig. 5). Normal and healed TM, regardless of acute or chronic perforation, showed low expression of the epithelial stem cell markers INGβ1 and CK19 in all areas of the TM. However, during the acute or chronic perforation state, INGβ1 and CK19 were up-regulated, especially in the areas around the malleus handle and the perforation side of the annulus. We also observed that expression of CK19 was much stronger and more sensitive than that of INGβ1, depending upon the TM state.


Latent progenitor cells as potential regulators for tympanic membrane regeneration.

Kim SW, Kim J, Seonwoo H, Jang KJ, Kim YJ, Lim HJ, Lim KT, Tian C, Chung JH, Choung YH - Sci Rep (2015)

The intensity of expression on potential stem cell makers (Integrin β1 (red color) and Cytokeratin 19 (blue color) according to the anatomic areas (malleus handle; annulus (perforation site); pars tensa; annulus (unperforation site) in the TMs with acute or chronic perforations.a) Acute TM perforation model. (b) Chronic TM perforation model.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477343&req=5

f5: The intensity of expression on potential stem cell makers (Integrin β1 (red color) and Cytokeratin 19 (blue color) according to the anatomic areas (malleus handle; annulus (perforation site); pars tensa; annulus (unperforation site) in the TMs with acute or chronic perforations.a) Acute TM perforation model. (b) Chronic TM perforation model.
Mentions: We quantitatively analyzed and compared the expression intensity of INGβ1 and CK19 in landmarked locations in the TM at different time points (Fig. 5). Normal and healed TM, regardless of acute or chronic perforation, showed low expression of the epithelial stem cell markers INGβ1 and CK19 in all areas of the TM. However, during the acute or chronic perforation state, INGβ1 and CK19 were up-regulated, especially in the areas around the malleus handle and the perforation side of the annulus. We also observed that expression of CK19 was much stronger and more sensitive than that of INGβ1, depending upon the TM state.

Bottom Line: Here, we explored an approach for TM regeneration where the latent progenitor or stem cells within TM epithelial layers may play an important regulatory role.Additionally, they are present at high levels in perforated TMs, especially in proximity to the holes, regardless of acute or chronic status, suggesting that TM stem cells may be a potential factor for TM regeneration.Our study suggests that latent TM stem cells could be potential regulators of regeneration, which provides a new insight into this clinically important process and a potential target for new therapies for chronic otitis media and other eardrum injuries.

View Article: PubMed Central - PubMed

Affiliation: Department of Burns and Plastic Surgery, Affiliated Hospital of Yanbian University, 1327 Juzi Street, Yanji, Jilin 133000, P.R. China.

ABSTRACT
Tympanic membrane (TM) perforation, in particular chronic otitis media, is one of the most common clinical problems in the world and can present with sensorineural healing loss. Here, we explored an approach for TM regeneration where the latent progenitor or stem cells within TM epithelial layers may play an important regulatory role. We showed that potential TM stem cells present highly positive staining for epithelial stem cell markers in all areas of normal TM tissue. Additionally, they are present at high levels in perforated TMs, especially in proximity to the holes, regardless of acute or chronic status, suggesting that TM stem cells may be a potential factor for TM regeneration. Our study suggests that latent TM stem cells could be potential regulators of regeneration, which provides a new insight into this clinically important process and a potential target for new therapies for chronic otitis media and other eardrum injuries.

No MeSH data available.


Related in: MedlinePlus