Limits...
Latent progenitor cells as potential regulators for tympanic membrane regeneration.

Kim SW, Kim J, Seonwoo H, Jang KJ, Kim YJ, Lim HJ, Lim KT, Tian C, Chung JH, Choung YH - Sci Rep (2015)

Bottom Line: Here, we explored an approach for TM regeneration where the latent progenitor or stem cells within TM epithelial layers may play an important regulatory role.Additionally, they are present at high levels in perforated TMs, especially in proximity to the holes, regardless of acute or chronic status, suggesting that TM stem cells may be a potential factor for TM regeneration.Our study suggests that latent TM stem cells could be potential regulators of regeneration, which provides a new insight into this clinically important process and a potential target for new therapies for chronic otitis media and other eardrum injuries.

View Article: PubMed Central - PubMed

Affiliation: Department of Burns and Plastic Surgery, Affiliated Hospital of Yanbian University, 1327 Juzi Street, Yanji, Jilin 133000, P.R. China.

ABSTRACT
Tympanic membrane (TM) perforation, in particular chronic otitis media, is one of the most common clinical problems in the world and can present with sensorineural healing loss. Here, we explored an approach for TM regeneration where the latent progenitor or stem cells within TM epithelial layers may play an important regulatory role. We showed that potential TM stem cells present highly positive staining for epithelial stem cell markers in all areas of normal TM tissue. Additionally, they are present at high levels in perforated TMs, especially in proximity to the holes, regardless of acute or chronic status, suggesting that TM stem cells may be a potential factor for TM regeneration. Our study suggests that latent TM stem cells could be potential regulators of regeneration, which provides a new insight into this clinically important process and a potential target for new therapies for chronic otitis media and other eardrum injuries.

No MeSH data available.


Related in: MedlinePlus

Expression of INGβ1 and CK19 in acute TM perforations.(a) TM at the first day after perforation, (b) TM at the fifth day after perforation. (c) TM at the tenth day after perforation. 1,5; annulus, 3; malleus handle, 2; pars tensa, 4; perforation site.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4477343&req=5

f3: Expression of INGβ1 and CK19 in acute TM perforations.(a) TM at the first day after perforation, (b) TM at the fifth day after perforation. (c) TM at the tenth day after perforation. 1,5; annulus, 3; malleus handle, 2; pars tensa, 4; perforation site.

Mentions: We analyzed the distribution of stem cells during regeneration of TMs after acute perforation using immunohistochemical staining for INGβ1 and CK19. After the TMs were perforated, we observed significantly higher expression of the epithelial stem cell markers INGβ1 and CK19 in all areas (Fig. 3a) compared to normal TMs (Fig. 2). The expression of INGβ1 and CK19 was more intense around the malleus handle and the annulus areas near the perforation holes (perforation sided annulus) than in the other areas (Fig. 3a). At 5 days after acute perforation, we observed that the lesion size was decreasing and the epithelial thickness was larger than that observed initially at 1 day, indicating that TM regeneration began spontaneously and immediately in acute perforations (Fig. 3b). Compared with the day 1, we observed that expression of INGβ1 and CK19 was significantly decreased in the non-perforated regions including the pars tensa and the annulus, whereas expression was still high in the perforated regions (Fig. 3b). This finding indicates that the regeneration potential was higher around perforation sites including the malleus handle and annulus areas. At 10 days, the TMs were completely healed and the expression of INGβ1 and CK19 was significantly reduced to a similar level to that in normal TMs (Fig. 3c).


Latent progenitor cells as potential regulators for tympanic membrane regeneration.

Kim SW, Kim J, Seonwoo H, Jang KJ, Kim YJ, Lim HJ, Lim KT, Tian C, Chung JH, Choung YH - Sci Rep (2015)

Expression of INGβ1 and CK19 in acute TM perforations.(a) TM at the first day after perforation, (b) TM at the fifth day after perforation. (c) TM at the tenth day after perforation. 1,5; annulus, 3; malleus handle, 2; pars tensa, 4; perforation site.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477343&req=5

f3: Expression of INGβ1 and CK19 in acute TM perforations.(a) TM at the first day after perforation, (b) TM at the fifth day after perforation. (c) TM at the tenth day after perforation. 1,5; annulus, 3; malleus handle, 2; pars tensa, 4; perforation site.
Mentions: We analyzed the distribution of stem cells during regeneration of TMs after acute perforation using immunohistochemical staining for INGβ1 and CK19. After the TMs were perforated, we observed significantly higher expression of the epithelial stem cell markers INGβ1 and CK19 in all areas (Fig. 3a) compared to normal TMs (Fig. 2). The expression of INGβ1 and CK19 was more intense around the malleus handle and the annulus areas near the perforation holes (perforation sided annulus) than in the other areas (Fig. 3a). At 5 days after acute perforation, we observed that the lesion size was decreasing and the epithelial thickness was larger than that observed initially at 1 day, indicating that TM regeneration began spontaneously and immediately in acute perforations (Fig. 3b). Compared with the day 1, we observed that expression of INGβ1 and CK19 was significantly decreased in the non-perforated regions including the pars tensa and the annulus, whereas expression was still high in the perforated regions (Fig. 3b). This finding indicates that the regeneration potential was higher around perforation sites including the malleus handle and annulus areas. At 10 days, the TMs were completely healed and the expression of INGβ1 and CK19 was significantly reduced to a similar level to that in normal TMs (Fig. 3c).

Bottom Line: Here, we explored an approach for TM regeneration where the latent progenitor or stem cells within TM epithelial layers may play an important regulatory role.Additionally, they are present at high levels in perforated TMs, especially in proximity to the holes, regardless of acute or chronic status, suggesting that TM stem cells may be a potential factor for TM regeneration.Our study suggests that latent TM stem cells could be potential regulators of regeneration, which provides a new insight into this clinically important process and a potential target for new therapies for chronic otitis media and other eardrum injuries.

View Article: PubMed Central - PubMed

Affiliation: Department of Burns and Plastic Surgery, Affiliated Hospital of Yanbian University, 1327 Juzi Street, Yanji, Jilin 133000, P.R. China.

ABSTRACT
Tympanic membrane (TM) perforation, in particular chronic otitis media, is one of the most common clinical problems in the world and can present with sensorineural healing loss. Here, we explored an approach for TM regeneration where the latent progenitor or stem cells within TM epithelial layers may play an important regulatory role. We showed that potential TM stem cells present highly positive staining for epithelial stem cell markers in all areas of normal TM tissue. Additionally, they are present at high levels in perforated TMs, especially in proximity to the holes, regardless of acute or chronic status, suggesting that TM stem cells may be a potential factor for TM regeneration. Our study suggests that latent TM stem cells could be potential regulators of regeneration, which provides a new insight into this clinically important process and a potential target for new therapies for chronic otitis media and other eardrum injuries.

No MeSH data available.


Related in: MedlinePlus