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Evidence for horizontal transfer of mitochondrial DNA to the plastid genome in a bamboo genus.

Ma PF, Zhang YX, Guo ZH, Li DZ - Sci Rep (2015)

Bottom Line: Here we sequenced 6 plastid genomes of bamboos, three of which are neotropical woody species and three are herbaceous ones.Our study provided evidence of the mitochondrial-to-plastid DNA transfer in the monocots, demonstrating again that this rare event does occur in other angiosperm lineages.However, the mechanism underlying the transfer remains obscure, and more studies in other plants may elucidate it in the future.

View Article: PubMed Central - PubMed

Affiliation: 1] Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China [2] Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China.

ABSTRACT
In flowering plants, three genomes (nuclear, mitochondrial, and plastid) coexist and intracellular horizontal transfer of DNA is prevalent, especially from the plastid to the mitochondrion genome. However, the plastid genomes are generally conserved in evolution and have long been considered immune to foreign DNA. Recently, the opposite direction of DNA transfer from the mitochondrial to the plastid genome has been reported in two eudicot lineages. Here we sequenced 6 plastid genomes of bamboos, three of which are neotropical woody species and three are herbaceous ones. Several unusual features were found, including the duplication of trnT-GGU and loss of one copy of rps19 due to contraction of inverted repeats (IRs). The most intriguing was the ~2.7 kb insertion in the plastid IR regions in the three herbaceous bamboos. Furthermore, the insertion was documented to be horizontally transferred from the mitochondrial to the plastid genome. Our study provided evidence of the mitochondrial-to-plastid DNA transfer in the monocots, demonstrating again that this rare event does occur in other angiosperm lineages. However, the mechanism underlying the transfer remains obscure, and more studies in other plants may elucidate it in the future.

No MeSH data available.


Related in: MedlinePlus

The mVISTA similarity plot of the insertion sequences in the plastid genomes of Pariana and Eremitis compared with the mitochondrial DNA sequence of F. rimosivaginus.The blank areas downstream from ~2.1 kb in E. parviflora and P. parvispica indicate undetermined sequence of insertion in these bamboos.
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f4: The mVISTA similarity plot of the insertion sequences in the plastid genomes of Pariana and Eremitis compared with the mitochondrial DNA sequence of F. rimosivaginus.The blank areas downstream from ~2.1 kb in E. parviflora and P. parvispica indicate undetermined sequence of insertion in these bamboos.

Mentions: The mVISTA29 alignment of the mitochondrial DNA sequence of F. rimosivaginus and the inserted sequences of 5 Parianinae species revealed a high global level of conservation with sequence identities only falling below 90% between positions approximately 600 and 750 (Fig. 4). Taken P. campestris as example, the inserted segment and the F. rimosivaginus mitochondrial genome differed by only 25 substitutions (transitions : transversions = 13 : 12) and 9 indels with a total length of 55 bp in an alignment of 2666 bp. Nevertheless, the sequences within 5000 bp upstream and downstream of the matching sequence in the F. rimosivaginus mitochondrial genome did not show any similarity to the plastid DNA sequences. The matching sequence is located in the intergenic region between the genes rps7 and atp6 in the mitochondrial genome of F. rimosivaginus27.


Evidence for horizontal transfer of mitochondrial DNA to the plastid genome in a bamboo genus.

Ma PF, Zhang YX, Guo ZH, Li DZ - Sci Rep (2015)

The mVISTA similarity plot of the insertion sequences in the plastid genomes of Pariana and Eremitis compared with the mitochondrial DNA sequence of F. rimosivaginus.The blank areas downstream from ~2.1 kb in E. parviflora and P. parvispica indicate undetermined sequence of insertion in these bamboos.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477325&req=5

f4: The mVISTA similarity plot of the insertion sequences in the plastid genomes of Pariana and Eremitis compared with the mitochondrial DNA sequence of F. rimosivaginus.The blank areas downstream from ~2.1 kb in E. parviflora and P. parvispica indicate undetermined sequence of insertion in these bamboos.
Mentions: The mVISTA29 alignment of the mitochondrial DNA sequence of F. rimosivaginus and the inserted sequences of 5 Parianinae species revealed a high global level of conservation with sequence identities only falling below 90% between positions approximately 600 and 750 (Fig. 4). Taken P. campestris as example, the inserted segment and the F. rimosivaginus mitochondrial genome differed by only 25 substitutions (transitions : transversions = 13 : 12) and 9 indels with a total length of 55 bp in an alignment of 2666 bp. Nevertheless, the sequences within 5000 bp upstream and downstream of the matching sequence in the F. rimosivaginus mitochondrial genome did not show any similarity to the plastid DNA sequences. The matching sequence is located in the intergenic region between the genes rps7 and atp6 in the mitochondrial genome of F. rimosivaginus27.

Bottom Line: Here we sequenced 6 plastid genomes of bamboos, three of which are neotropical woody species and three are herbaceous ones.Our study provided evidence of the mitochondrial-to-plastid DNA transfer in the monocots, demonstrating again that this rare event does occur in other angiosperm lineages.However, the mechanism underlying the transfer remains obscure, and more studies in other plants may elucidate it in the future.

View Article: PubMed Central - PubMed

Affiliation: 1] Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China [2] Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China.

ABSTRACT
In flowering plants, three genomes (nuclear, mitochondrial, and plastid) coexist and intracellular horizontal transfer of DNA is prevalent, especially from the plastid to the mitochondrion genome. However, the plastid genomes are generally conserved in evolution and have long been considered immune to foreign DNA. Recently, the opposite direction of DNA transfer from the mitochondrial to the plastid genome has been reported in two eudicot lineages. Here we sequenced 6 plastid genomes of bamboos, three of which are neotropical woody species and three are herbaceous ones. Several unusual features were found, including the duplication of trnT-GGU and loss of one copy of rps19 due to contraction of inverted repeats (IRs). The most intriguing was the ~2.7 kb insertion in the plastid IR regions in the three herbaceous bamboos. Furthermore, the insertion was documented to be horizontally transferred from the mitochondrial to the plastid genome. Our study provided evidence of the mitochondrial-to-plastid DNA transfer in the monocots, demonstrating again that this rare event does occur in other angiosperm lineages. However, the mechanism underlying the transfer remains obscure, and more studies in other plants may elucidate it in the future.

No MeSH data available.


Related in: MedlinePlus