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Improvement of antioxidant status after Brazil nut intake in hypertensive and dyslipidemic subjects.

Huguenin GV, Oliveira GM, Moreira AS, Saint'Pierre TD, Gonçalves RA, Pinheiro-Mulder AR, Teodoro AJ, Luiz RR, Rosa G - Nutr J (2015)

Bottom Line: GBN intake significantly increased plasma Se from 87.0 ± 16.8 to 180.6 ± 67.1 μg/L, increased GPx3 activity in 24,8% (from 112.66 ± 40.09 to 128.32 ± 38.31 nmol/min/mL, p < 0,05), and reduced 3.25% of oxidized-LDL levels (from 66.31 ± 23.59 to 60.68 ± 20.88 U/L, p < 0.05).There wasn't association between GPx3 and 8-epi PGF2α (β -0.209, p = 0.052) by simple model.The partially defatted GBN intake has a potential benefit to increase plasma selenium, increase enzymatic antioxidant activity of GPx3 and to reduction oxidation in LDL in hypertensive and dyslipidemic patients.

View Article: PubMed Central - PubMed

Affiliation: Institute of Heart Edson Saad, Federal Universityof Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil. grazielle.huguenin@gmail.com.

ABSTRACT

Objectives: To investigate the effect of partially defatted Granulated Brazil nut (GBN) on biomarkers of oxidative stress and antioxidant status of hypertensive and dyslipidemic patients on nutrition and drug approaches.

Methods: Ninety one hypertensive and dyslipidemic subjects of both genders (51.6 % men), mean age 62.1 ± 9.3 years, performed a randomized crossover trial, double-blind, placebo controlled. Subjects received a diet and partially defatted GBN 13 g per day (≈227.5 μg/day of selenium) or placebo for twelve weeks with four-week washout interval. Anthropometric, laboratory and clinic characteristics were investigated at baseline. Plasma selenium (Se), plasma glutathione peroxidase (GPx3) activity, total antioxidant capacity (TAC), 8-epi PGF2α and oxidized LDL were evaluated at the beginning and in the end of each intervention.

Results: GBN intake significantly increased plasma Se from 87.0 ± 16.8 to 180.6 ± 67.1 μg/L, increased GPx3 activity in 24,8% (from 112.66 ± 40.09 to 128.32 ± 38.31 nmol/min/mL, p < 0,05), and reduced 3.25% of oxidized-LDL levels (from 66.31 ± 23.59 to 60.68 ± 20.88 U/L, p < 0.05). An inverse association between GPx3 and oxidized LDL levels was observed after supplementation with GBN by simple model (β -0.232, p = 0.032) and after adjustment for gender, age, diabetes and BMI (β -0.298, p = 0.008). There wasn't association between GPx3 and 8-epi PGF2α (β -0.209, p = 0.052) by simple model.

Conclusion: The partially defatted GBN intake has a potential benefit to increase plasma selenium, increase enzymatic antioxidant activity of GPx3 and to reduction oxidation in LDL in hypertensive and dyslipidemic patients.

Trial registration: ClinicalTrials.gov Identifier NCT01990391; November 20, 2013.

No MeSH data available.


Related in: MedlinePlus

Association between GPx3 activity and biomarkers of oxidative stress. Simple linear regression. a. Granulated Brazil nut (p = 0.032) and Placebo (p = 0.371); b. Granulated Brazil nut (p = 0.052) e Placebo (p = 0.836)
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Fig4: Association between GPx3 activity and biomarkers of oxidative stress. Simple linear regression. a. Granulated Brazil nut (p = 0.032) and Placebo (p = 0.371); b. Granulated Brazil nut (p = 0.052) e Placebo (p = 0.836)

Mentions: A significant inverse association between oxidized-LDL and GPx3 activity after Granulated Brazil nut intake is shown in Fig. 4 with a simple linear regression model (B − 0.434, IC 95 % − 0.829;−0039, β − 0.232, p = 0.032) and it remained significant after adjustment for age, BMI, gender, diabetes and smoking status (B − 0.556, IC 95 % − 0.963;−0148, β − 0.298, p = 0.008). However no statistical significance was observed between 8-epi PGF2α and GPx3 activity with the simple linear regression model (B − 0.991, IC 95 % − 1.991;−0009, β − 0.209, p = 0.052) as shown in Fig. 4. Nevertheless, a significant inverse association between 8-epi PGF2α and GPx3 activity was achieved after adjustment for age, BMI, gender and diabetes (B − 1.082, IC 95 % − 2.081;−0.084, β − 0.234, p = 0.034).Fig. 4


Improvement of antioxidant status after Brazil nut intake in hypertensive and dyslipidemic subjects.

Huguenin GV, Oliveira GM, Moreira AS, Saint'Pierre TD, Gonçalves RA, Pinheiro-Mulder AR, Teodoro AJ, Luiz RR, Rosa G - Nutr J (2015)

Association between GPx3 activity and biomarkers of oxidative stress. Simple linear regression. a. Granulated Brazil nut (p = 0.032) and Placebo (p = 0.371); b. Granulated Brazil nut (p = 0.052) e Placebo (p = 0.836)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4477321&req=5

Fig4: Association between GPx3 activity and biomarkers of oxidative stress. Simple linear regression. a. Granulated Brazil nut (p = 0.032) and Placebo (p = 0.371); b. Granulated Brazil nut (p = 0.052) e Placebo (p = 0.836)
Mentions: A significant inverse association between oxidized-LDL and GPx3 activity after Granulated Brazil nut intake is shown in Fig. 4 with a simple linear regression model (B − 0.434, IC 95 % − 0.829;−0039, β − 0.232, p = 0.032) and it remained significant after adjustment for age, BMI, gender, diabetes and smoking status (B − 0.556, IC 95 % − 0.963;−0148, β − 0.298, p = 0.008). However no statistical significance was observed between 8-epi PGF2α and GPx3 activity with the simple linear regression model (B − 0.991, IC 95 % − 1.991;−0009, β − 0.209, p = 0.052) as shown in Fig. 4. Nevertheless, a significant inverse association between 8-epi PGF2α and GPx3 activity was achieved after adjustment for age, BMI, gender and diabetes (B − 1.082, IC 95 % − 2.081;−0.084, β − 0.234, p = 0.034).Fig. 4

Bottom Line: GBN intake significantly increased plasma Se from 87.0 ± 16.8 to 180.6 ± 67.1 μg/L, increased GPx3 activity in 24,8% (from 112.66 ± 40.09 to 128.32 ± 38.31 nmol/min/mL, p < 0,05), and reduced 3.25% of oxidized-LDL levels (from 66.31 ± 23.59 to 60.68 ± 20.88 U/L, p < 0.05).There wasn't association between GPx3 and 8-epi PGF2α (β -0.209, p = 0.052) by simple model.The partially defatted GBN intake has a potential benefit to increase plasma selenium, increase enzymatic antioxidant activity of GPx3 and to reduction oxidation in LDL in hypertensive and dyslipidemic patients.

View Article: PubMed Central - PubMed

Affiliation: Institute of Heart Edson Saad, Federal Universityof Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil. grazielle.huguenin@gmail.com.

ABSTRACT

Objectives: To investigate the effect of partially defatted Granulated Brazil nut (GBN) on biomarkers of oxidative stress and antioxidant status of hypertensive and dyslipidemic patients on nutrition and drug approaches.

Methods: Ninety one hypertensive and dyslipidemic subjects of both genders (51.6 % men), mean age 62.1 ± 9.3 years, performed a randomized crossover trial, double-blind, placebo controlled. Subjects received a diet and partially defatted GBN 13 g per day (≈227.5 μg/day of selenium) or placebo for twelve weeks with four-week washout interval. Anthropometric, laboratory and clinic characteristics were investigated at baseline. Plasma selenium (Se), plasma glutathione peroxidase (GPx3) activity, total antioxidant capacity (TAC), 8-epi PGF2α and oxidized LDL were evaluated at the beginning and in the end of each intervention.

Results: GBN intake significantly increased plasma Se from 87.0 ± 16.8 to 180.6 ± 67.1 μg/L, increased GPx3 activity in 24,8% (from 112.66 ± 40.09 to 128.32 ± 38.31 nmol/min/mL, p < 0,05), and reduced 3.25% of oxidized-LDL levels (from 66.31 ± 23.59 to 60.68 ± 20.88 U/L, p < 0.05). An inverse association between GPx3 and oxidized LDL levels was observed after supplementation with GBN by simple model (β -0.232, p = 0.032) and after adjustment for gender, age, diabetes and BMI (β -0.298, p = 0.008). There wasn't association between GPx3 and 8-epi PGF2α (β -0.209, p = 0.052) by simple model.

Conclusion: The partially defatted GBN intake has a potential benefit to increase plasma selenium, increase enzymatic antioxidant activity of GPx3 and to reduction oxidation in LDL in hypertensive and dyslipidemic patients.

Trial registration: ClinicalTrials.gov Identifier NCT01990391; November 20, 2013.

No MeSH data available.


Related in: MedlinePlus