Limits...
Improvement of antioxidant status after Brazil nut intake in hypertensive and dyslipidemic subjects.

Huguenin GV, Oliveira GM, Moreira AS, Saint'Pierre TD, Gonçalves RA, Pinheiro-Mulder AR, Teodoro AJ, Luiz RR, Rosa G - Nutr J (2015)

Bottom Line: GBN intake significantly increased plasma Se from 87.0 ± 16.8 to 180.6 ± 67.1 μg/L, increased GPx3 activity in 24,8% (from 112.66 ± 40.09 to 128.32 ± 38.31 nmol/min/mL, p < 0,05), and reduced 3.25% of oxidized-LDL levels (from 66.31 ± 23.59 to 60.68 ± 20.88 U/L, p < 0.05).There wasn't association between GPx3 and 8-epi PGF2α (β -0.209, p = 0.052) by simple model.The partially defatted GBN intake has a potential benefit to increase plasma selenium, increase enzymatic antioxidant activity of GPx3 and to reduction oxidation in LDL in hypertensive and dyslipidemic patients.

View Article: PubMed Central - PubMed

Affiliation: Institute of Heart Edson Saad, Federal Universityof Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil. grazielle.huguenin@gmail.com.

ABSTRACT

Objectives: To investigate the effect of partially defatted Granulated Brazil nut (GBN) on biomarkers of oxidative stress and antioxidant status of hypertensive and dyslipidemic patients on nutrition and drug approaches.

Methods: Ninety one hypertensive and dyslipidemic subjects of both genders (51.6 % men), mean age 62.1 ± 9.3 years, performed a randomized crossover trial, double-blind, placebo controlled. Subjects received a diet and partially defatted GBN 13 g per day (≈227.5 μg/day of selenium) or placebo for twelve weeks with four-week washout interval. Anthropometric, laboratory and clinic characteristics were investigated at baseline. Plasma selenium (Se), plasma glutathione peroxidase (GPx3) activity, total antioxidant capacity (TAC), 8-epi PGF2α and oxidized LDL were evaluated at the beginning and in the end of each intervention.

Results: GBN intake significantly increased plasma Se from 87.0 ± 16.8 to 180.6 ± 67.1 μg/L, increased GPx3 activity in 24,8% (from 112.66 ± 40.09 to 128.32 ± 38.31 nmol/min/mL, p < 0,05), and reduced 3.25% of oxidized-LDL levels (from 66.31 ± 23.59 to 60.68 ± 20.88 U/L, p < 0.05). An inverse association between GPx3 and oxidized LDL levels was observed after supplementation with GBN by simple model (β -0.232, p = 0.032) and after adjustment for gender, age, diabetes and BMI (β -0.298, p = 0.008). There wasn't association between GPx3 and 8-epi PGF2α (β -0.209, p = 0.052) by simple model.

Conclusion: The partially defatted GBN intake has a potential benefit to increase plasma selenium, increase enzymatic antioxidant activity of GPx3 and to reduction oxidation in LDL in hypertensive and dyslipidemic patients.

Trial registration: ClinicalTrials.gov Identifier NCT01990391; November 20, 2013.

No MeSH data available.


Related in: MedlinePlus

Plasma Se levels during intervention with Brazil nut, placebo and washout period. Data are expressed as the mean ± SD. Student’s t-test between placebo and Granulated Brazil nut groups. * p < 0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4477321&req=5

Fig2: Plasma Se levels during intervention with Brazil nut, placebo and washout period. Data are expressed as the mean ± SD. Student’s t-test between placebo and Granulated Brazil nut groups. * p < 0.05

Mentions: Basal plasma Se concentration from the study group was 87.0 ± 16.8 μg/L and it was observed that 57.1 % (n = 52) presented low plasma Se (<90 μg/L). Throughout intervention, Se plasma concentrations increased significantly with the *Brazil nut intake (Fig. 2). It has been observed an overall increase in Plasma Se by 119 %. However, it was possible to verify the carry over effect after the washout period on placebo diet Se concentrations; this occurred because part 112of the study group who received Granulated Brazil nut supplementation on the first steps did not return to the same basal values, making plasma Se initial value to be elevated in relation to Granulated Brazil nut supplementation initial value.Fig. 2


Improvement of antioxidant status after Brazil nut intake in hypertensive and dyslipidemic subjects.

Huguenin GV, Oliveira GM, Moreira AS, Saint'Pierre TD, Gonçalves RA, Pinheiro-Mulder AR, Teodoro AJ, Luiz RR, Rosa G - Nutr J (2015)

Plasma Se levels during intervention with Brazil nut, placebo and washout period. Data are expressed as the mean ± SD. Student’s t-test between placebo and Granulated Brazil nut groups. * p < 0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4477321&req=5

Fig2: Plasma Se levels during intervention with Brazil nut, placebo and washout period. Data are expressed as the mean ± SD. Student’s t-test between placebo and Granulated Brazil nut groups. * p < 0.05
Mentions: Basal plasma Se concentration from the study group was 87.0 ± 16.8 μg/L and it was observed that 57.1 % (n = 52) presented low plasma Se (<90 μg/L). Throughout intervention, Se plasma concentrations increased significantly with the *Brazil nut intake (Fig. 2). It has been observed an overall increase in Plasma Se by 119 %. However, it was possible to verify the carry over effect after the washout period on placebo diet Se concentrations; this occurred because part 112of the study group who received Granulated Brazil nut supplementation on the first steps did not return to the same basal values, making plasma Se initial value to be elevated in relation to Granulated Brazil nut supplementation initial value.Fig. 2

Bottom Line: GBN intake significantly increased plasma Se from 87.0 ± 16.8 to 180.6 ± 67.1 μg/L, increased GPx3 activity in 24,8% (from 112.66 ± 40.09 to 128.32 ± 38.31 nmol/min/mL, p < 0,05), and reduced 3.25% of oxidized-LDL levels (from 66.31 ± 23.59 to 60.68 ± 20.88 U/L, p < 0.05).There wasn't association between GPx3 and 8-epi PGF2α (β -0.209, p = 0.052) by simple model.The partially defatted GBN intake has a potential benefit to increase plasma selenium, increase enzymatic antioxidant activity of GPx3 and to reduction oxidation in LDL in hypertensive and dyslipidemic patients.

View Article: PubMed Central - PubMed

Affiliation: Institute of Heart Edson Saad, Federal Universityof Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil. grazielle.huguenin@gmail.com.

ABSTRACT

Objectives: To investigate the effect of partially defatted Granulated Brazil nut (GBN) on biomarkers of oxidative stress and antioxidant status of hypertensive and dyslipidemic patients on nutrition and drug approaches.

Methods: Ninety one hypertensive and dyslipidemic subjects of both genders (51.6 % men), mean age 62.1 ± 9.3 years, performed a randomized crossover trial, double-blind, placebo controlled. Subjects received a diet and partially defatted GBN 13 g per day (≈227.5 μg/day of selenium) or placebo for twelve weeks with four-week washout interval. Anthropometric, laboratory and clinic characteristics were investigated at baseline. Plasma selenium (Se), plasma glutathione peroxidase (GPx3) activity, total antioxidant capacity (TAC), 8-epi PGF2α and oxidized LDL were evaluated at the beginning and in the end of each intervention.

Results: GBN intake significantly increased plasma Se from 87.0 ± 16.8 to 180.6 ± 67.1 μg/L, increased GPx3 activity in 24,8% (from 112.66 ± 40.09 to 128.32 ± 38.31 nmol/min/mL, p < 0,05), and reduced 3.25% of oxidized-LDL levels (from 66.31 ± 23.59 to 60.68 ± 20.88 U/L, p < 0.05). An inverse association between GPx3 and oxidized LDL levels was observed after supplementation with GBN by simple model (β -0.232, p = 0.032) and after adjustment for gender, age, diabetes and BMI (β -0.298, p = 0.008). There wasn't association between GPx3 and 8-epi PGF2α (β -0.209, p = 0.052) by simple model.

Conclusion: The partially defatted GBN intake has a potential benefit to increase plasma selenium, increase enzymatic antioxidant activity of GPx3 and to reduction oxidation in LDL in hypertensive and dyslipidemic patients.

Trial registration: ClinicalTrials.gov Identifier NCT01990391; November 20, 2013.

No MeSH data available.


Related in: MedlinePlus