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Tiliacora triandra, an Anti-Intoxication Plant, Improves Memory Impairment, Neurodegeneration, Cholinergic Function, and Oxidative Stress in Hippocampus of Ethanol Dependence Rats.

Phunchago N, Wattanathorn J, Chaisiwamongkol K - Oxid Med Cell Longev (2015)

Bottom Line: The extract also mitigated the decreased retention time, SOD, CAT, and GSH-Px activities, and neurons density in hippocampus induced by alcohol.Therefore, T. triandra is the potential reagent for treating brain dysfunction induced by alcohol.However, further researches are necessary to understand the detail mechanism and possible active ingredient.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology (Neuroscience Program) and Graduate School, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand ; Integrative Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen 40002, Thailand.

ABSTRACT
Oxidative stress plays an important role in brain dysfunctions induced by alcohol. Since less therapeutic agent against cognitive deficit and brain damage induced by chronic alcohol consumption is less available, we aimed to assess the effect of Tiliacora triandra extract, a plant possessing antioxidant activity, on memory impairment, neuron density, cholinergic function, and oxidative stress in hippocampus of alcoholic rats. Male Wistar rats were induced ethanol dependence condition by semivoluntary intake of alcohol for 15 weeks. Alcoholic rats were orally given T. triandra at doses of 100, 200, and 400 mg·kg(-1)BW for 14 days. Memory assessment was performed every 7 days while neuron density, activities of AChE, SOD, CAT, and GSH-Px and, MDA level in hippocampus were assessed at the end of study. Interestingly, the extract mitigated the increased escape latency, AChE and MDA level. The extract also mitigated the decreased retention time, SOD, CAT, and GSH-Px activities, and neurons density in hippocampus induced by alcohol. These data suggested that the extract improved memory deficit in alcoholic rats partly via the decreased oxidative stress and the suppression of AChE. Therefore, T. triandra is the potential reagent for treating brain dysfunction induced by alcohol. However, further researches are necessary to understand the detail mechanism and possible active ingredient.

No MeSH data available.


Related in: MedlinePlus

The effect of T. triandra extract on the neuron density in CA1, CA2, CA3, and dentate gyrus of hippocampus. (a) Photographs showing the density of the neurons stained with cresyl violet; (b) the bar graph showing density of neurons in various subregions of hippocampus. Data were presented as mean ± SEM, n = 6/group. ###P value < 0.001 compared with control treated group. ∗∗∗P value < 0.001 compared with ethanol dependence treated group which received vehicle.
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fig5: The effect of T. triandra extract on the neuron density in CA1, CA2, CA3, and dentate gyrus of hippocampus. (a) Photographs showing the density of the neurons stained with cresyl violet; (b) the bar graph showing density of neurons in various subregions of hippocampus. Data were presented as mean ± SEM, n = 6/group. ###P value < 0.001 compared with control treated group. ∗∗∗P value < 0.001 compared with ethanol dependence treated group which received vehicle.

Mentions: Effect of T. triandra on neuron density in various subregions of hippocampus including CA1, CA2, CA3, and dentate gyrus was shown in Figures 5(a) and 5(b). Repetitive consumption of alcohol significantly decreased neuron density in CA1, CA2, CA3, and dentate gyrus of hippocampus (P value < 0.001 all, compared with control rats). Ethanol dependence rats which received either donepezil or vitamin C significantly attenuated the decreased neuron density induced by alcohol in all subregions mentioned earlier of hippocampus (P value < 0.01 all, compared with ethanol dependence rats which received vehicle). Interestingly, all doses of T. triandra extract treatment significantly counteracted the decreased neuron density induced by alcohol consumption in hippocampus (P value < 0.01 all, compared with ethanol dependence rats which received vehicle).


Tiliacora triandra, an Anti-Intoxication Plant, Improves Memory Impairment, Neurodegeneration, Cholinergic Function, and Oxidative Stress in Hippocampus of Ethanol Dependence Rats.

Phunchago N, Wattanathorn J, Chaisiwamongkol K - Oxid Med Cell Longev (2015)

The effect of T. triandra extract on the neuron density in CA1, CA2, CA3, and dentate gyrus of hippocampus. (a) Photographs showing the density of the neurons stained with cresyl violet; (b) the bar graph showing density of neurons in various subregions of hippocampus. Data were presented as mean ± SEM, n = 6/group. ###P value < 0.001 compared with control treated group. ∗∗∗P value < 0.001 compared with ethanol dependence treated group which received vehicle.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477251&req=5

fig5: The effect of T. triandra extract on the neuron density in CA1, CA2, CA3, and dentate gyrus of hippocampus. (a) Photographs showing the density of the neurons stained with cresyl violet; (b) the bar graph showing density of neurons in various subregions of hippocampus. Data were presented as mean ± SEM, n = 6/group. ###P value < 0.001 compared with control treated group. ∗∗∗P value < 0.001 compared with ethanol dependence treated group which received vehicle.
Mentions: Effect of T. triandra on neuron density in various subregions of hippocampus including CA1, CA2, CA3, and dentate gyrus was shown in Figures 5(a) and 5(b). Repetitive consumption of alcohol significantly decreased neuron density in CA1, CA2, CA3, and dentate gyrus of hippocampus (P value < 0.001 all, compared with control rats). Ethanol dependence rats which received either donepezil or vitamin C significantly attenuated the decreased neuron density induced by alcohol in all subregions mentioned earlier of hippocampus (P value < 0.01 all, compared with ethanol dependence rats which received vehicle). Interestingly, all doses of T. triandra extract treatment significantly counteracted the decreased neuron density induced by alcohol consumption in hippocampus (P value < 0.01 all, compared with ethanol dependence rats which received vehicle).

Bottom Line: The extract also mitigated the decreased retention time, SOD, CAT, and GSH-Px activities, and neurons density in hippocampus induced by alcohol.Therefore, T. triandra is the potential reagent for treating brain dysfunction induced by alcohol.However, further researches are necessary to understand the detail mechanism and possible active ingredient.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology (Neuroscience Program) and Graduate School, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand ; Integrative Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen 40002, Thailand.

ABSTRACT
Oxidative stress plays an important role in brain dysfunctions induced by alcohol. Since less therapeutic agent against cognitive deficit and brain damage induced by chronic alcohol consumption is less available, we aimed to assess the effect of Tiliacora triandra extract, a plant possessing antioxidant activity, on memory impairment, neuron density, cholinergic function, and oxidative stress in hippocampus of alcoholic rats. Male Wistar rats were induced ethanol dependence condition by semivoluntary intake of alcohol for 15 weeks. Alcoholic rats were orally given T. triandra at doses of 100, 200, and 400 mg·kg(-1)BW for 14 days. Memory assessment was performed every 7 days while neuron density, activities of AChE, SOD, CAT, and GSH-Px and, MDA level in hippocampus were assessed at the end of study. Interestingly, the extract mitigated the increased escape latency, AChE and MDA level. The extract also mitigated the decreased retention time, SOD, CAT, and GSH-Px activities, and neurons density in hippocampus induced by alcohol. These data suggested that the extract improved memory deficit in alcoholic rats partly via the decreased oxidative stress and the suppression of AChE. Therefore, T. triandra is the potential reagent for treating brain dysfunction induced by alcohol. However, further researches are necessary to understand the detail mechanism and possible active ingredient.

No MeSH data available.


Related in: MedlinePlus