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Tiliacora triandra, an Anti-Intoxication Plant, Improves Memory Impairment, Neurodegeneration, Cholinergic Function, and Oxidative Stress in Hippocampus of Ethanol Dependence Rats.

Phunchago N, Wattanathorn J, Chaisiwamongkol K - Oxid Med Cell Longev (2015)

Bottom Line: The extract also mitigated the decreased retention time, SOD, CAT, and GSH-Px activities, and neurons density in hippocampus induced by alcohol.Therefore, T. triandra is the potential reagent for treating brain dysfunction induced by alcohol.However, further researches are necessary to understand the detail mechanism and possible active ingredient.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology (Neuroscience Program) and Graduate School, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand ; Integrative Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen 40002, Thailand.

ABSTRACT
Oxidative stress plays an important role in brain dysfunctions induced by alcohol. Since less therapeutic agent against cognitive deficit and brain damage induced by chronic alcohol consumption is less available, we aimed to assess the effect of Tiliacora triandra extract, a plant possessing antioxidant activity, on memory impairment, neuron density, cholinergic function, and oxidative stress in hippocampus of alcoholic rats. Male Wistar rats were induced ethanol dependence condition by semivoluntary intake of alcohol for 15 weeks. Alcoholic rats were orally given T. triandra at doses of 100, 200, and 400 mg·kg(-1)BW for 14 days. Memory assessment was performed every 7 days while neuron density, activities of AChE, SOD, CAT, and GSH-Px and, MDA level in hippocampus were assessed at the end of study. Interestingly, the extract mitigated the increased escape latency, AChE and MDA level. The extract also mitigated the decreased retention time, SOD, CAT, and GSH-Px activities, and neurons density in hippocampus induced by alcohol. These data suggested that the extract improved memory deficit in alcoholic rats partly via the decreased oxidative stress and the suppression of AChE. Therefore, T. triandra is the potential reagent for treating brain dysfunction induced by alcohol. However, further researches are necessary to understand the detail mechanism and possible active ingredient.

No MeSH data available.


Related in: MedlinePlus

The chromatogram of water extract of T. triandra.
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fig1: The chromatogram of water extract of T. triandra.

Mentions: The data obtained from this study showed that the T. triandra leaves extract used in this study contained total phenolic compounds at concentration of 593.33 mg of gallic acid equivalent (GAE)/mg extract. The HPLC fingerprint of T. triandra was shown in Figure 1. We had identified gallic acid, cyanidin, and quercetin according to their retention times and spectral characteristics of their peaks compared with standard. The ultraviolet spectrum of chromatographic bands presented in the fingerprinting of the samples indicated the presence of gallic acid at a concentration of 4.81 ± 0.05 μg gallic/100 mg of extract whereas the concentrations of cyanidins and quercetin were presented at the concentrations of 307.22 ± 4.74 μg Cyn-3-glu/100 mg and 9028.86 ± 695.97 μg QE/100 mg extract, respectively.


Tiliacora triandra, an Anti-Intoxication Plant, Improves Memory Impairment, Neurodegeneration, Cholinergic Function, and Oxidative Stress in Hippocampus of Ethanol Dependence Rats.

Phunchago N, Wattanathorn J, Chaisiwamongkol K - Oxid Med Cell Longev (2015)

The chromatogram of water extract of T. triandra.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477251&req=5

fig1: The chromatogram of water extract of T. triandra.
Mentions: The data obtained from this study showed that the T. triandra leaves extract used in this study contained total phenolic compounds at concentration of 593.33 mg of gallic acid equivalent (GAE)/mg extract. The HPLC fingerprint of T. triandra was shown in Figure 1. We had identified gallic acid, cyanidin, and quercetin according to their retention times and spectral characteristics of their peaks compared with standard. The ultraviolet spectrum of chromatographic bands presented in the fingerprinting of the samples indicated the presence of gallic acid at a concentration of 4.81 ± 0.05 μg gallic/100 mg of extract whereas the concentrations of cyanidins and quercetin were presented at the concentrations of 307.22 ± 4.74 μg Cyn-3-glu/100 mg and 9028.86 ± 695.97 μg QE/100 mg extract, respectively.

Bottom Line: The extract also mitigated the decreased retention time, SOD, CAT, and GSH-Px activities, and neurons density in hippocampus induced by alcohol.Therefore, T. triandra is the potential reagent for treating brain dysfunction induced by alcohol.However, further researches are necessary to understand the detail mechanism and possible active ingredient.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology (Neuroscience Program) and Graduate School, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand ; Integrative Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen 40002, Thailand.

ABSTRACT
Oxidative stress plays an important role in brain dysfunctions induced by alcohol. Since less therapeutic agent against cognitive deficit and brain damage induced by chronic alcohol consumption is less available, we aimed to assess the effect of Tiliacora triandra extract, a plant possessing antioxidant activity, on memory impairment, neuron density, cholinergic function, and oxidative stress in hippocampus of alcoholic rats. Male Wistar rats were induced ethanol dependence condition by semivoluntary intake of alcohol for 15 weeks. Alcoholic rats were orally given T. triandra at doses of 100, 200, and 400 mg·kg(-1)BW for 14 days. Memory assessment was performed every 7 days while neuron density, activities of AChE, SOD, CAT, and GSH-Px and, MDA level in hippocampus were assessed at the end of study. Interestingly, the extract mitigated the increased escape latency, AChE and MDA level. The extract also mitigated the decreased retention time, SOD, CAT, and GSH-Px activities, and neurons density in hippocampus induced by alcohol. These data suggested that the extract improved memory deficit in alcoholic rats partly via the decreased oxidative stress and the suppression of AChE. Therefore, T. triandra is the potential reagent for treating brain dysfunction induced by alcohol. However, further researches are necessary to understand the detail mechanism and possible active ingredient.

No MeSH data available.


Related in: MedlinePlus