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Doses of Quercetin in the Range of Serum Concentrations Exert Delipidating Effects in 3T3-L1 Preadipocytes by Acting on Different Stages of Adipogenesis, but Not in Mature Adipocytes.

Eseberri I, Miranda J, Lasa A, Churruca I, Portillo MP - Oxid Med Cell Longev (2015)

Bottom Line: In mature adipocytes, only 10 µM of quercetin reduced triacylglycerol content.Lipogenic FAS expression and activity were reduced at this dose.Quercetin, in the range of serum concentrations, is able to inhibit adipogenesis, but higher doses, at least 10 µM, are needed to reduce fat accumulation in mature adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Institute, University of País Vasco (UPV/EHU), 01006 Vitoria, Spain ; CIBEROBN Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), 01006 Vitoria, Spain.

ABSTRACT

Scope: To determine whether doses of quercetin in the range of serum concentrations exert any effect on triacylglycerol accumulation in maturing preadipocytes and mature adipocytes. The influence on the expression of adipogenic markers as well as on gene expression and activity of enzymes involved in triacylglycerol metabolism were assessed.

Methods and results: 3T3-L1 preadipocytes were treated during differentiation and mature adipocytes for 24 hours with low doses (0.1-10 µM) of quercetin. Triacylglycerol content in both cell types and free fatty acid and glycerol in the incubation medium of mature adipocytes were measured spectrophotometrically. Gene and protein expression were assessed by RT-PCR and Western blot. LPL and FAS activities were quantified. During differentiation quercetin reduced triacylglycerol content at doses from 0.5 to 10 µM. 1 µM of quercetin reduced C/EBPβ gene expression, SREBP1 mature protein levels, and PPARγ gene expression. 10 µM of quercetin reduced LPL gene expression and PPARγ and SREBP1c expression. In mature adipocytes, only 10 µM of quercetin reduced triacylglycerol content. Lipogenic FAS expression and activity were reduced at this dose.

Conclusion: Quercetin, in the range of serum concentrations, is able to inhibit adipogenesis, but higher doses, at least 10 µM, are needed to reduce fat accumulation in mature adipocytes.

No MeSH data available.


Effects of different doses of quercetin in the pathways of the adipogenic process.
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fig6: Effects of different doses of quercetin in the pathways of the adipogenic process.

Mentions: Two phases can be distinguished in adipogenesis, the premitotic phase (early stage of differentiation; 60 hours after confluence) [28] and the postmitotic phase (late stage). During the early phase C/EBPβ and SREBP1c expressions are increased. This change, in turn, triggers high level expression of PPARγ, which is considered the master coordinator of adipocyte differentiation. C/EBPα and LPL are induced during later stage of differentiation [58]. Interestingly, when the influence of quercetin on the expression of these genes was assessed it was observed that, depending on the dose, the mechanisms of action of this flavonoid to reduce adipogenesis were different (Figure 6). Quercetin, at a dose of 1 μM, significantly reduced C/EBPβ and PPARγ gene expressions, but PPARγ1 and PPARγ2 protein levels remained unchanged. Moreover, a reduction in protein expression of mature SREBP1c was observed. As stated before, C/EBPβ and SREBP1c are adipogenic markers expressed in the early stage of the process [28]. Taken together these results suggest that adipogenesis was stopped at this phase. This fact was confirmed by the reduction in TG content (tendency) observed after 60 hours of treatment, but not when preadipocytes were treated from 60 hours to 8 days.


Doses of Quercetin in the Range of Serum Concentrations Exert Delipidating Effects in 3T3-L1 Preadipocytes by Acting on Different Stages of Adipogenesis, but Not in Mature Adipocytes.

Eseberri I, Miranda J, Lasa A, Churruca I, Portillo MP - Oxid Med Cell Longev (2015)

Effects of different doses of quercetin in the pathways of the adipogenic process.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477249&req=5

fig6: Effects of different doses of quercetin in the pathways of the adipogenic process.
Mentions: Two phases can be distinguished in adipogenesis, the premitotic phase (early stage of differentiation; 60 hours after confluence) [28] and the postmitotic phase (late stage). During the early phase C/EBPβ and SREBP1c expressions are increased. This change, in turn, triggers high level expression of PPARγ, which is considered the master coordinator of adipocyte differentiation. C/EBPα and LPL are induced during later stage of differentiation [58]. Interestingly, when the influence of quercetin on the expression of these genes was assessed it was observed that, depending on the dose, the mechanisms of action of this flavonoid to reduce adipogenesis were different (Figure 6). Quercetin, at a dose of 1 μM, significantly reduced C/EBPβ and PPARγ gene expressions, but PPARγ1 and PPARγ2 protein levels remained unchanged. Moreover, a reduction in protein expression of mature SREBP1c was observed. As stated before, C/EBPβ and SREBP1c are adipogenic markers expressed in the early stage of the process [28]. Taken together these results suggest that adipogenesis was stopped at this phase. This fact was confirmed by the reduction in TG content (tendency) observed after 60 hours of treatment, but not when preadipocytes were treated from 60 hours to 8 days.

Bottom Line: In mature adipocytes, only 10 µM of quercetin reduced triacylglycerol content.Lipogenic FAS expression and activity were reduced at this dose.Quercetin, in the range of serum concentrations, is able to inhibit adipogenesis, but higher doses, at least 10 µM, are needed to reduce fat accumulation in mature adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Institute, University of País Vasco (UPV/EHU), 01006 Vitoria, Spain ; CIBEROBN Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), 01006 Vitoria, Spain.

ABSTRACT

Scope: To determine whether doses of quercetin in the range of serum concentrations exert any effect on triacylglycerol accumulation in maturing preadipocytes and mature adipocytes. The influence on the expression of adipogenic markers as well as on gene expression and activity of enzymes involved in triacylglycerol metabolism were assessed.

Methods and results: 3T3-L1 preadipocytes were treated during differentiation and mature adipocytes for 24 hours with low doses (0.1-10 µM) of quercetin. Triacylglycerol content in both cell types and free fatty acid and glycerol in the incubation medium of mature adipocytes were measured spectrophotometrically. Gene and protein expression were assessed by RT-PCR and Western blot. LPL and FAS activities were quantified. During differentiation quercetin reduced triacylglycerol content at doses from 0.5 to 10 µM. 1 µM of quercetin reduced C/EBPβ gene expression, SREBP1 mature protein levels, and PPARγ gene expression. 10 µM of quercetin reduced LPL gene expression and PPARγ and SREBP1c expression. In mature adipocytes, only 10 µM of quercetin reduced triacylglycerol content. Lipogenic FAS expression and activity were reduced at this dose.

Conclusion: Quercetin, in the range of serum concentrations, is able to inhibit adipogenesis, but higher doses, at least 10 µM, are needed to reduce fat accumulation in mature adipocytes.

No MeSH data available.