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Doses of Quercetin in the Range of Serum Concentrations Exert Delipidating Effects in 3T3-L1 Preadipocytes by Acting on Different Stages of Adipogenesis, but Not in Mature Adipocytes.

Eseberri I, Miranda J, Lasa A, Churruca I, Portillo MP - Oxid Med Cell Longev (2015)

Bottom Line: In mature adipocytes, only 10 µM of quercetin reduced triacylglycerol content.Lipogenic FAS expression and activity were reduced at this dose.Quercetin, in the range of serum concentrations, is able to inhibit adipogenesis, but higher doses, at least 10 µM, are needed to reduce fat accumulation in mature adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Institute, University of País Vasco (UPV/EHU), 01006 Vitoria, Spain ; CIBEROBN Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), 01006 Vitoria, Spain.

ABSTRACT

Scope: To determine whether doses of quercetin in the range of serum concentrations exert any effect on triacylglycerol accumulation in maturing preadipocytes and mature adipocytes. The influence on the expression of adipogenic markers as well as on gene expression and activity of enzymes involved in triacylglycerol metabolism were assessed.

Methods and results: 3T3-L1 preadipocytes were treated during differentiation and mature adipocytes for 24 hours with low doses (0.1-10 µM) of quercetin. Triacylglycerol content in both cell types and free fatty acid and glycerol in the incubation medium of mature adipocytes were measured spectrophotometrically. Gene and protein expression were assessed by RT-PCR and Western blot. LPL and FAS activities were quantified. During differentiation quercetin reduced triacylglycerol content at doses from 0.5 to 10 µM. 1 µM of quercetin reduced C/EBPβ gene expression, SREBP1 mature protein levels, and PPARγ gene expression. 10 µM of quercetin reduced LPL gene expression and PPARγ and SREBP1c expression. In mature adipocytes, only 10 µM of quercetin reduced triacylglycerol content. Lipogenic FAS expression and activity were reduced at this dose.

Conclusion: Quercetin, in the range of serum concentrations, is able to inhibit adipogenesis, but higher doses, at least 10 µM, are needed to reduce fat accumulation in mature adipocytes.

No MeSH data available.


Related in: MedlinePlus

Effects of 10 μM of quercetin (Q10) on the gene expression of lipases, ATGL and HSL, in 3T3-L1 mature adipocytes treated for 24 h. Values are means ± SEM. Comparisons between each treatment and the controls were analyzed by Student's t-test. The asterisks represent differences versus the controls (**P < 0.01).
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fig2: Effects of 10 μM of quercetin (Q10) on the gene expression of lipases, ATGL and HSL, in 3T3-L1 mature adipocytes treated for 24 h. Values are means ± SEM. Comparisons between each treatment and the controls were analyzed by Student's t-test. The asterisks represent differences versus the controls (**P < 0.01).

Mentions: In mature adipocytes, gene expression was analyzed only in cells treated with 10 μM of quercetin because this dose was the only effective one. With regard to lipolytic enzymes, HSL expression was significantly reduced and ATGL remained unchanged (Figure 2). FASN expression was significantly reduced, but no changes were observed in ACC and LPL (Figure 3(a)).


Doses of Quercetin in the Range of Serum Concentrations Exert Delipidating Effects in 3T3-L1 Preadipocytes by Acting on Different Stages of Adipogenesis, but Not in Mature Adipocytes.

Eseberri I, Miranda J, Lasa A, Churruca I, Portillo MP - Oxid Med Cell Longev (2015)

Effects of 10 μM of quercetin (Q10) on the gene expression of lipases, ATGL and HSL, in 3T3-L1 mature adipocytes treated for 24 h. Values are means ± SEM. Comparisons between each treatment and the controls were analyzed by Student's t-test. The asterisks represent differences versus the controls (**P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477249&req=5

fig2: Effects of 10 μM of quercetin (Q10) on the gene expression of lipases, ATGL and HSL, in 3T3-L1 mature adipocytes treated for 24 h. Values are means ± SEM. Comparisons between each treatment and the controls were analyzed by Student's t-test. The asterisks represent differences versus the controls (**P < 0.01).
Mentions: In mature adipocytes, gene expression was analyzed only in cells treated with 10 μM of quercetin because this dose was the only effective one. With regard to lipolytic enzymes, HSL expression was significantly reduced and ATGL remained unchanged (Figure 2). FASN expression was significantly reduced, but no changes were observed in ACC and LPL (Figure 3(a)).

Bottom Line: In mature adipocytes, only 10 µM of quercetin reduced triacylglycerol content.Lipogenic FAS expression and activity were reduced at this dose.Quercetin, in the range of serum concentrations, is able to inhibit adipogenesis, but higher doses, at least 10 µM, are needed to reduce fat accumulation in mature adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Institute, University of País Vasco (UPV/EHU), 01006 Vitoria, Spain ; CIBEROBN Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), 01006 Vitoria, Spain.

ABSTRACT

Scope: To determine whether doses of quercetin in the range of serum concentrations exert any effect on triacylglycerol accumulation in maturing preadipocytes and mature adipocytes. The influence on the expression of adipogenic markers as well as on gene expression and activity of enzymes involved in triacylglycerol metabolism were assessed.

Methods and results: 3T3-L1 preadipocytes were treated during differentiation and mature adipocytes for 24 hours with low doses (0.1-10 µM) of quercetin. Triacylglycerol content in both cell types and free fatty acid and glycerol in the incubation medium of mature adipocytes were measured spectrophotometrically. Gene and protein expression were assessed by RT-PCR and Western blot. LPL and FAS activities were quantified. During differentiation quercetin reduced triacylglycerol content at doses from 0.5 to 10 µM. 1 µM of quercetin reduced C/EBPβ gene expression, SREBP1 mature protein levels, and PPARγ gene expression. 10 µM of quercetin reduced LPL gene expression and PPARγ and SREBP1c expression. In mature adipocytes, only 10 µM of quercetin reduced triacylglycerol content. Lipogenic FAS expression and activity were reduced at this dose.

Conclusion: Quercetin, in the range of serum concentrations, is able to inhibit adipogenesis, but higher doses, at least 10 µM, are needed to reduce fat accumulation in mature adipocytes.

No MeSH data available.


Related in: MedlinePlus