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Antioxidative Effects and Inhibition of Human Low Density Lipoprotein Oxidation In Vitro of Polyphenolic Compounds in Flammulina velutipes (Golden Needle Mushroom).

Rahman MA, Abdullah N, Aminudin N - Oxid Med Cell Longev (2015)

Bottom Line: In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated.M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction.These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes.

View Article: PubMed Central - PubMed

Affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1340, Bangladesh.

ABSTRACT
Dietary polyphenolic compounds mediate polynomial actions in guarding against multiple diseases. Atherosclerosis is an oxidative stress driven pathophysiological complication where free radical induced oxidative modification of low density lipoprotein (LDL) plays the ground breaking role. Mushrooms have been highly regarded for possessing an antioxidant arsenal. Polyphenolic compounds present in dietary mushrooms seem pertinent in withstanding LDL oxidation en route to controlling atherosclerosis. In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated. M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction. In LDL oxidation inhibitory tests, M : DCM fraction at 1 µg/mL concentration mostly lengthened the lag time (125 mins) of conjugated diene formation and inhibited the formation of thiobarbituric acid reactive substances (48.71%, at 1 mg/mL concentration). LC-MS/MS analyses of M : DCM fraction identified the presence of polyphenolic substances protocatechuic acid, p-coumaric, and ellagic acid. These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes. Thus, mushroom-based dietary polyphenolic compounds might be implicated in slowing down the progression of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus

TBARS formation inhibitory effect of F. velutipes fractions as compared to positive control, quercetin. Data presented as mean ± SD of triplicate determinations. Mean values with different lowercase superscripts (a–f) represent statistically significant difference at 95% level (P ≤ 0.05) with post hoc least significance difference (LSD) test.
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fig5: TBARS formation inhibitory effect of F. velutipes fractions as compared to positive control, quercetin. Data presented as mean ± SD of triplicate determinations. Mean values with different lowercase superscripts (a–f) represent statistically significant difference at 95% level (P ≤ 0.05) with post hoc least significance difference (LSD) test.

Mentions: During LDL oxidation, the lag phase is followed by the propagation phase of rapid LDL oxidation giving rise to lipid peroxides. Lipid peroxides undergo decomposition phase when breaking of the double bonds gives rise to malondialdehydes (MDA). Nucleophilic substitution reaction between MDA and TBA used in the experimentation produced “MDA:TBA adduct,” also called “thiobarbituric acid reactive substances (TBARS)” [29]. The amount of TBARS production is inversely proportional to the antioxidant capacity of a biocomponent. All the solvent fractions of F. velutipes were capable of inhibiting the formation of TBARS, but the M : DCM fraction inhibited it the most (48.71%) (Figure 5).


Antioxidative Effects and Inhibition of Human Low Density Lipoprotein Oxidation In Vitro of Polyphenolic Compounds in Flammulina velutipes (Golden Needle Mushroom).

Rahman MA, Abdullah N, Aminudin N - Oxid Med Cell Longev (2015)

TBARS formation inhibitory effect of F. velutipes fractions as compared to positive control, quercetin. Data presented as mean ± SD of triplicate determinations. Mean values with different lowercase superscripts (a–f) represent statistically significant difference at 95% level (P ≤ 0.05) with post hoc least significance difference (LSD) test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477244&req=5

fig5: TBARS formation inhibitory effect of F. velutipes fractions as compared to positive control, quercetin. Data presented as mean ± SD of triplicate determinations. Mean values with different lowercase superscripts (a–f) represent statistically significant difference at 95% level (P ≤ 0.05) with post hoc least significance difference (LSD) test.
Mentions: During LDL oxidation, the lag phase is followed by the propagation phase of rapid LDL oxidation giving rise to lipid peroxides. Lipid peroxides undergo decomposition phase when breaking of the double bonds gives rise to malondialdehydes (MDA). Nucleophilic substitution reaction between MDA and TBA used in the experimentation produced “MDA:TBA adduct,” also called “thiobarbituric acid reactive substances (TBARS)” [29]. The amount of TBARS production is inversely proportional to the antioxidant capacity of a biocomponent. All the solvent fractions of F. velutipes were capable of inhibiting the formation of TBARS, but the M : DCM fraction inhibited it the most (48.71%) (Figure 5).

Bottom Line: In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated.M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction.These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes.

View Article: PubMed Central - PubMed

Affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1340, Bangladesh.

ABSTRACT
Dietary polyphenolic compounds mediate polynomial actions in guarding against multiple diseases. Atherosclerosis is an oxidative stress driven pathophysiological complication where free radical induced oxidative modification of low density lipoprotein (LDL) plays the ground breaking role. Mushrooms have been highly regarded for possessing an antioxidant arsenal. Polyphenolic compounds present in dietary mushrooms seem pertinent in withstanding LDL oxidation en route to controlling atherosclerosis. In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated. M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction. In LDL oxidation inhibitory tests, M : DCM fraction at 1 µg/mL concentration mostly lengthened the lag time (125 mins) of conjugated diene formation and inhibited the formation of thiobarbituric acid reactive substances (48.71%, at 1 mg/mL concentration). LC-MS/MS analyses of M : DCM fraction identified the presence of polyphenolic substances protocatechuic acid, p-coumaric, and ellagic acid. These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes. Thus, mushroom-based dietary polyphenolic compounds might be implicated in slowing down the progression of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus