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Antioxidative Effects and Inhibition of Human Low Density Lipoprotein Oxidation In Vitro of Polyphenolic Compounds in Flammulina velutipes (Golden Needle Mushroom).

Rahman MA, Abdullah N, Aminudin N - Oxid Med Cell Longev (2015)

Bottom Line: In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated.M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction.These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes.

View Article: PubMed Central - PubMed

Affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1340, Bangladesh.

ABSTRACT
Dietary polyphenolic compounds mediate polynomial actions in guarding against multiple diseases. Atherosclerosis is an oxidative stress driven pathophysiological complication where free radical induced oxidative modification of low density lipoprotein (LDL) plays the ground breaking role. Mushrooms have been highly regarded for possessing an antioxidant arsenal. Polyphenolic compounds present in dietary mushrooms seem pertinent in withstanding LDL oxidation en route to controlling atherosclerosis. In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated. M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction. In LDL oxidation inhibitory tests, M : DCM fraction at 1 µg/mL concentration mostly lengthened the lag time (125 mins) of conjugated diene formation and inhibited the formation of thiobarbituric acid reactive substances (48.71%, at 1 mg/mL concentration). LC-MS/MS analyses of M : DCM fraction identified the presence of polyphenolic substances protocatechuic acid, p-coumaric, and ellagic acid. These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes. Thus, mushroom-based dietary polyphenolic compounds might be implicated in slowing down the progression of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus

Effect of F. velutipes fractions upon lag time of conjugated diene (CD) formation. The gradual increase in the lag time of CD formation at 234 nm indicates the extent of ox-LDL inhibition by the respective fraction against the FeSO4-induced LDL oxidation. The positive control, quercetin, and all the fractions were used at 1 μg/mL conc.
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fig4: Effect of F. velutipes fractions upon lag time of conjugated diene (CD) formation. The gradual increase in the lag time of CD formation at 234 nm indicates the extent of ox-LDL inhibition by the respective fraction against the FeSO4-induced LDL oxidation. The positive control, quercetin, and all the fractions were used at 1 μg/mL conc.

Mentions: Fe+2-ions exerted peroxidative modification of the polyunsaturated fatty acids (PUFAs) present in the LDL molecule and caused molecular rearrangement, resulting in conjugated dienes (CDs) formation. As the LDL molecule's integral antioxidant, α-tocopherol protects LDL by withstanding the oxidative stress initially, causing a slowed oxidation, known as the lag period. Oxidation withstanding prowess of an antioxidant is directly proportional to the lengthening of the lag phase [14]. When F. velutipes solvent partitioned fractions were applied, the lag time was lengthened even up to 120 minutes for the M : DCM and 95 minutes for the hexane fraction, respectively (Figure 4).


Antioxidative Effects and Inhibition of Human Low Density Lipoprotein Oxidation In Vitro of Polyphenolic Compounds in Flammulina velutipes (Golden Needle Mushroom).

Rahman MA, Abdullah N, Aminudin N - Oxid Med Cell Longev (2015)

Effect of F. velutipes fractions upon lag time of conjugated diene (CD) formation. The gradual increase in the lag time of CD formation at 234 nm indicates the extent of ox-LDL inhibition by the respective fraction against the FeSO4-induced LDL oxidation. The positive control, quercetin, and all the fractions were used at 1 μg/mL conc.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477244&req=5

fig4: Effect of F. velutipes fractions upon lag time of conjugated diene (CD) formation. The gradual increase in the lag time of CD formation at 234 nm indicates the extent of ox-LDL inhibition by the respective fraction against the FeSO4-induced LDL oxidation. The positive control, quercetin, and all the fractions were used at 1 μg/mL conc.
Mentions: Fe+2-ions exerted peroxidative modification of the polyunsaturated fatty acids (PUFAs) present in the LDL molecule and caused molecular rearrangement, resulting in conjugated dienes (CDs) formation. As the LDL molecule's integral antioxidant, α-tocopherol protects LDL by withstanding the oxidative stress initially, causing a slowed oxidation, known as the lag period. Oxidation withstanding prowess of an antioxidant is directly proportional to the lengthening of the lag phase [14]. When F. velutipes solvent partitioned fractions were applied, the lag time was lengthened even up to 120 minutes for the M : DCM and 95 minutes for the hexane fraction, respectively (Figure 4).

Bottom Line: In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated.M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction.These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes.

View Article: PubMed Central - PubMed

Affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1340, Bangladesh.

ABSTRACT
Dietary polyphenolic compounds mediate polynomial actions in guarding against multiple diseases. Atherosclerosis is an oxidative stress driven pathophysiological complication where free radical induced oxidative modification of low density lipoprotein (LDL) plays the ground breaking role. Mushrooms have been highly regarded for possessing an antioxidant arsenal. Polyphenolic compounds present in dietary mushrooms seem pertinent in withstanding LDL oxidation en route to controlling atherosclerosis. In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated. M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction. In LDL oxidation inhibitory tests, M : DCM fraction at 1 µg/mL concentration mostly lengthened the lag time (125 mins) of conjugated diene formation and inhibited the formation of thiobarbituric acid reactive substances (48.71%, at 1 mg/mL concentration). LC-MS/MS analyses of M : DCM fraction identified the presence of polyphenolic substances protocatechuic acid, p-coumaric, and ellagic acid. These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes. Thus, mushroom-based dietary polyphenolic compounds might be implicated in slowing down the progression of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus