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Antioxidative Effects and Inhibition of Human Low Density Lipoprotein Oxidation In Vitro of Polyphenolic Compounds in Flammulina velutipes (Golden Needle Mushroom).

Rahman MA, Abdullah N, Aminudin N - Oxid Med Cell Longev (2015)

Bottom Line: In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated.M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction.These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes.

View Article: PubMed Central - PubMed

Affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1340, Bangladesh.

ABSTRACT
Dietary polyphenolic compounds mediate polynomial actions in guarding against multiple diseases. Atherosclerosis is an oxidative stress driven pathophysiological complication where free radical induced oxidative modification of low density lipoprotein (LDL) plays the ground breaking role. Mushrooms have been highly regarded for possessing an antioxidant arsenal. Polyphenolic compounds present in dietary mushrooms seem pertinent in withstanding LDL oxidation en route to controlling atherosclerosis. In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated. M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction. In LDL oxidation inhibitory tests, M : DCM fraction at 1 µg/mL concentration mostly lengthened the lag time (125 mins) of conjugated diene formation and inhibited the formation of thiobarbituric acid reactive substances (48.71%, at 1 mg/mL concentration). LC-MS/MS analyses of M : DCM fraction identified the presence of polyphenolic substances protocatechuic acid, p-coumaric, and ellagic acid. These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes. Thus, mushroom-based dietary polyphenolic compounds might be implicated in slowing down the progression of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus

DPPH free radical scavenging effect of the F. velutipes fractions compared to the positive control, quercetin. Data presented as mean ± SD of triplicate determinations. Mean values with different lowercase superscripts (a–f) represent statistically significant difference at 95% level (P ≤ 0.05) with post hoc least significance difference (LSD) test.
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fig1: DPPH free radical scavenging effect of the F. velutipes fractions compared to the positive control, quercetin. Data presented as mean ± SD of triplicate determinations. Mean values with different lowercase superscripts (a–f) represent statistically significant difference at 95% level (P ≤ 0.05) with post hoc least significance difference (LSD) test.

Mentions: The antioxidant property of natural compounds was most commonly evaluated on the basis of DPPH free radical scavenging property. Although the test is not any single antioxidant-specific but rather measures the cumulative antioxidant potential of any sample, the procedure is simple, rapid, and cost effective. Specifically, the scavenging antioxidants reduce the stable, purple radical DPPH∙ through bond pairing between its odd electron and scavenging antioxidant's hydrogen atom and convert it into yellow colored, nonradical form (DPPH-H). As shown in Figure 1, at 1 mg/mL concentration, the methyl : DCM fraction of F. velutipes performed the best DPPH free radical scavenging effect (60.75%, IC50 0.86 mg/mL).


Antioxidative Effects and Inhibition of Human Low Density Lipoprotein Oxidation In Vitro of Polyphenolic Compounds in Flammulina velutipes (Golden Needle Mushroom).

Rahman MA, Abdullah N, Aminudin N - Oxid Med Cell Longev (2015)

DPPH free radical scavenging effect of the F. velutipes fractions compared to the positive control, quercetin. Data presented as mean ± SD of triplicate determinations. Mean values with different lowercase superscripts (a–f) represent statistically significant difference at 95% level (P ≤ 0.05) with post hoc least significance difference (LSD) test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477244&req=5

fig1: DPPH free radical scavenging effect of the F. velutipes fractions compared to the positive control, quercetin. Data presented as mean ± SD of triplicate determinations. Mean values with different lowercase superscripts (a–f) represent statistically significant difference at 95% level (P ≤ 0.05) with post hoc least significance difference (LSD) test.
Mentions: The antioxidant property of natural compounds was most commonly evaluated on the basis of DPPH free radical scavenging property. Although the test is not any single antioxidant-specific but rather measures the cumulative antioxidant potential of any sample, the procedure is simple, rapid, and cost effective. Specifically, the scavenging antioxidants reduce the stable, purple radical DPPH∙ through bond pairing between its odd electron and scavenging antioxidant's hydrogen atom and convert it into yellow colored, nonradical form (DPPH-H). As shown in Figure 1, at 1 mg/mL concentration, the methyl : DCM fraction of F. velutipes performed the best DPPH free radical scavenging effect (60.75%, IC50 0.86 mg/mL).

Bottom Line: In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated.M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction.These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes.

View Article: PubMed Central - PubMed

Affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1340, Bangladesh.

ABSTRACT
Dietary polyphenolic compounds mediate polynomial actions in guarding against multiple diseases. Atherosclerosis is an oxidative stress driven pathophysiological complication where free radical induced oxidative modification of low density lipoprotein (LDL) plays the ground breaking role. Mushrooms have been highly regarded for possessing an antioxidant arsenal. Polyphenolic compounds present in dietary mushrooms seem pertinent in withstanding LDL oxidation en route to controlling atherosclerosis. In this study, the antioxidative effect of five solvent fractions consisting of methanol : dichloromethane (M : DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and aqueous residue (AQ) of Flammulina velutipes was evaluated. M : DCM fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect with IC50 of 0.86 mg/mL and total phenolic content of 56.36 gallic acid equivalent/g fraction. In LDL oxidation inhibitory tests, M : DCM fraction at 1 µg/mL concentration mostly lengthened the lag time (125 mins) of conjugated diene formation and inhibited the formation of thiobarbituric acid reactive substances (48.71%, at 1 mg/mL concentration). LC-MS/MS analyses of M : DCM fraction identified the presence of polyphenolic substances protocatechuic acid, p-coumaric, and ellagic acid. These chain-breaking polyphenolics might impart the antioxidative effects of F. velutipes. Thus, mushroom-based dietary polyphenolic compounds might be implicated in slowing down the progression of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus