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Flaxseed Oil Containing α -Linolenic Acid Ester of Plant Sterol Improved Atherosclerosis in ApoE Deficient Mice.

Han H, Yan P, Chen L, Luo C, Gao H, Deng Q, Zheng M, Shi Y, Liu L - Oxid Med Cell Longev (2015)

Bottom Line: Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties.Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress.These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

ABSTRACT
Plant sterols (PS) have potential preventive function in atherosclerosis due to their cholesterol-lowering ability. Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties. This study was designed to evaluate the effects of flaxseed oil containing α-linolenic acid ester of PS (ALA-PS) on atherosclerosis and investigate the underlying mechanisms. C57BL/6 mice were administered a regular diet and apoE knockout (apoE-KO) mice were given a high fat diet alone or supplemented with 5% flaxseed oil with or without 3.3% ALA-PS for 18 weeks. Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress. These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

No MeSH data available.


Related in: MedlinePlus

Effects of FO+ALA-PS on aortic ROS production and the levels of MDA and GSH in serum and liver of mice. ROS in the aorta of the mice was detected by using DHE which reacts with ROS and forms ETH that binds to DNA and produces red fluorescence signal, visualized with fluorescence microscope (×200) and quantified (a). Fluorescence intensities in randomly selected areas of the images were quantified by using the IPP image analysis software. Values are given as mean ± standard deviation of the mean (n = 3) (b). Values are means for serum MDA level (c), liver MDA level (d), serum GSH level (e), and liver GSH level (f) (n = 14) with standard deviations represented by vertical bars. aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group.
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fig7: Effects of FO+ALA-PS on aortic ROS production and the levels of MDA and GSH in serum and liver of mice. ROS in the aorta of the mice was detected by using DHE which reacts with ROS and forms ETH that binds to DNA and produces red fluorescence signal, visualized with fluorescence microscope (×200) and quantified (a). Fluorescence intensities in randomly selected areas of the images were quantified by using the IPP image analysis software. Values are given as mean ± standard deviation of the mean (n = 3) (b). Values are means for serum MDA level (c), liver MDA level (d), serum GSH level (e), and liver GSH level (f) (n = 14) with standard deviations represented by vertical bars. aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group.

Mentions: Recently acquired evidence pointed out that oxidative stress is a primary contributor to atherosclerosis. In this experiment, in situ ROS production of aorta was determined. As illustrated in Figures 7(a) and 7(b), high level of ROS in aorta induced by HFD was significantly reduced after exposed to flaxseed oil, and this depression effect was further improved by adding ALA-PS. Moreover, FO+ALA-PS but not FO consumption apparently decreased the concentrations of MDA and elevated the levels of GSH in serum and liver (Figures 7(c)–7(f)). To examine the potential molecular signaling pathways of FO+ALA-PS against oxidative stress, the main subunits of nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase contributing to atherosclerosis were detected. In mice given FO+ALA-PS supplemented diet, mRNA and protein expressions of aortic p22phox, p47phox, p67phox, and gp91phox were apparently less than those of HFD-fed mice (Figure 8).


Flaxseed Oil Containing α -Linolenic Acid Ester of Plant Sterol Improved Atherosclerosis in ApoE Deficient Mice.

Han H, Yan P, Chen L, Luo C, Gao H, Deng Q, Zheng M, Shi Y, Liu L - Oxid Med Cell Longev (2015)

Effects of FO+ALA-PS on aortic ROS production and the levels of MDA and GSH in serum and liver of mice. ROS in the aorta of the mice was detected by using DHE which reacts with ROS and forms ETH that binds to DNA and produces red fluorescence signal, visualized with fluorescence microscope (×200) and quantified (a). Fluorescence intensities in randomly selected areas of the images were quantified by using the IPP image analysis software. Values are given as mean ± standard deviation of the mean (n = 3) (b). Values are means for serum MDA level (c), liver MDA level (d), serum GSH level (e), and liver GSH level (f) (n = 14) with standard deviations represented by vertical bars. aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477243&req=5

fig7: Effects of FO+ALA-PS on aortic ROS production and the levels of MDA and GSH in serum and liver of mice. ROS in the aorta of the mice was detected by using DHE which reacts with ROS and forms ETH that binds to DNA and produces red fluorescence signal, visualized with fluorescence microscope (×200) and quantified (a). Fluorescence intensities in randomly selected areas of the images were quantified by using the IPP image analysis software. Values are given as mean ± standard deviation of the mean (n = 3) (b). Values are means for serum MDA level (c), liver MDA level (d), serum GSH level (e), and liver GSH level (f) (n = 14) with standard deviations represented by vertical bars. aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group.
Mentions: Recently acquired evidence pointed out that oxidative stress is a primary contributor to atherosclerosis. In this experiment, in situ ROS production of aorta was determined. As illustrated in Figures 7(a) and 7(b), high level of ROS in aorta induced by HFD was significantly reduced after exposed to flaxseed oil, and this depression effect was further improved by adding ALA-PS. Moreover, FO+ALA-PS but not FO consumption apparently decreased the concentrations of MDA and elevated the levels of GSH in serum and liver (Figures 7(c)–7(f)). To examine the potential molecular signaling pathways of FO+ALA-PS against oxidative stress, the main subunits of nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase contributing to atherosclerosis were detected. In mice given FO+ALA-PS supplemented diet, mRNA and protein expressions of aortic p22phox, p47phox, p67phox, and gp91phox were apparently less than those of HFD-fed mice (Figure 8).

Bottom Line: Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties.Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress.These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

ABSTRACT
Plant sterols (PS) have potential preventive function in atherosclerosis due to their cholesterol-lowering ability. Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties. This study was designed to evaluate the effects of flaxseed oil containing α-linolenic acid ester of PS (ALA-PS) on atherosclerosis and investigate the underlying mechanisms. C57BL/6 mice were administered a regular diet and apoE knockout (apoE-KO) mice were given a high fat diet alone or supplemented with 5% flaxseed oil with or without 3.3% ALA-PS for 18 weeks. Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress. These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

No MeSH data available.


Related in: MedlinePlus