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Flaxseed Oil Containing α -Linolenic Acid Ester of Plant Sterol Improved Atherosclerosis in ApoE Deficient Mice.

Han H, Yan P, Chen L, Luo C, Gao H, Deng Q, Zheng M, Shi Y, Liu L - Oxid Med Cell Longev (2015)

Bottom Line: Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties.Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress.These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

ABSTRACT
Plant sterols (PS) have potential preventive function in atherosclerosis due to their cholesterol-lowering ability. Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties. This study was designed to evaluate the effects of flaxseed oil containing α-linolenic acid ester of PS (ALA-PS) on atherosclerosis and investigate the underlying mechanisms. C57BL/6 mice were administered a regular diet and apoE knockout (apoE-KO) mice were given a high fat diet alone or supplemented with 5% flaxseed oil with or without 3.3% ALA-PS for 18 weeks. Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress. These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

No MeSH data available.


Related in: MedlinePlus

Treatment of FO+ALA-PS prevents atherosclerotic development via anti-inflammatory and antiproliferative actions. Representative images of aortic cross-sections immunostained with markers of macrophage infiltration, inflammation, and proliferation from mice fed a normal, HFD, FO, or ALA-PS diet for 18 wk. Immunoreactivity to MAC (a), Ly-6C (b), and SMC (c) antibodies are evident with brownish diaminobenzidine staining (magnification ×400).
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fig2: Treatment of FO+ALA-PS prevents atherosclerotic development via anti-inflammatory and antiproliferative actions. Representative images of aortic cross-sections immunostained with markers of macrophage infiltration, inflammation, and proliferation from mice fed a normal, HFD, FO, or ALA-PS diet for 18 wk. Immunoreactivity to MAC (a), Ly-6C (b), and SMC (c) antibodies are evident with brownish diaminobenzidine staining (magnification ×400).

Mentions: Inflammation is now considered to be an important mechanistic process involved in atherosclerosis, and markers of inflammation are also detected as a function of the dietary interventions. The macrophage marker CD68 and Ly-6C have been used as an indicator of inflammatory reactions associated with atherosclerosis. As shown in Figures 2(a) and 2(b), mice fed HFD were characterized by increased infiltration of macrophages and Ly-6C monocyte compared with control. Flaxseed oil supplementation alone did not reduce macrophages, but when included with ALA-PS, flaxseed oil was capable of mitigating the accumulation of macrophages.


Flaxseed Oil Containing α -Linolenic Acid Ester of Plant Sterol Improved Atherosclerosis in ApoE Deficient Mice.

Han H, Yan P, Chen L, Luo C, Gao H, Deng Q, Zheng M, Shi Y, Liu L - Oxid Med Cell Longev (2015)

Treatment of FO+ALA-PS prevents atherosclerotic development via anti-inflammatory and antiproliferative actions. Representative images of aortic cross-sections immunostained with markers of macrophage infiltration, inflammation, and proliferation from mice fed a normal, HFD, FO, or ALA-PS diet for 18 wk. Immunoreactivity to MAC (a), Ly-6C (b), and SMC (c) antibodies are evident with brownish diaminobenzidine staining (magnification ×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477243&req=5

fig2: Treatment of FO+ALA-PS prevents atherosclerotic development via anti-inflammatory and antiproliferative actions. Representative images of aortic cross-sections immunostained with markers of macrophage infiltration, inflammation, and proliferation from mice fed a normal, HFD, FO, or ALA-PS diet for 18 wk. Immunoreactivity to MAC (a), Ly-6C (b), and SMC (c) antibodies are evident with brownish diaminobenzidine staining (magnification ×400).
Mentions: Inflammation is now considered to be an important mechanistic process involved in atherosclerosis, and markers of inflammation are also detected as a function of the dietary interventions. The macrophage marker CD68 and Ly-6C have been used as an indicator of inflammatory reactions associated with atherosclerosis. As shown in Figures 2(a) and 2(b), mice fed HFD were characterized by increased infiltration of macrophages and Ly-6C monocyte compared with control. Flaxseed oil supplementation alone did not reduce macrophages, but when included with ALA-PS, flaxseed oil was capable of mitigating the accumulation of macrophages.

Bottom Line: Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties.Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress.These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

ABSTRACT
Plant sterols (PS) have potential preventive function in atherosclerosis due to their cholesterol-lowering ability. Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties. This study was designed to evaluate the effects of flaxseed oil containing α-linolenic acid ester of PS (ALA-PS) on atherosclerosis and investigate the underlying mechanisms. C57BL/6 mice were administered a regular diet and apoE knockout (apoE-KO) mice were given a high fat diet alone or supplemented with 5% flaxseed oil with or without 3.3% ALA-PS for 18 weeks. Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress. These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

No MeSH data available.


Related in: MedlinePlus