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Flaxseed Oil Containing α -Linolenic Acid Ester of Plant Sterol Improved Atherosclerosis in ApoE Deficient Mice.

Han H, Yan P, Chen L, Luo C, Gao H, Deng Q, Zheng M, Shi Y, Liu L - Oxid Med Cell Longev (2015)

Bottom Line: Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties.Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress.These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

ABSTRACT
Plant sterols (PS) have potential preventive function in atherosclerosis due to their cholesterol-lowering ability. Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties. This study was designed to evaluate the effects of flaxseed oil containing α-linolenic acid ester of PS (ALA-PS) on atherosclerosis and investigate the underlying mechanisms. C57BL/6 mice were administered a regular diet and apoE knockout (apoE-KO) mice were given a high fat diet alone or supplemented with 5% flaxseed oil with or without 3.3% ALA-PS for 18 weeks. Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress. These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

No MeSH data available.


Related in: MedlinePlus

Intervention of FO+ALA-PS ameliorated atherosclerosis in HFD diet-fed mice. Photomicrographs of representative Oil-Red O stained aortas of mice in control, HFD, FO, and ALA-PS groups (a). Quantitative analysis of the atherosclerotic lesion aortas of mice. The lesion area was measured as the percentage of aortic luminal area covered by atherosclerotic lesions. Values are given as mean ± standard deviation of the mean (n = 3). aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group (b). Representative images of cross-sections taken of the aortic sinuses obtained from mice in control, HFD, FO, and ALA-PS groups. The sections of aortic sinuses were stained with Oil-Red O (magnification ×40) for lipid deposition (red) and cross-stained with hematoxylin (blue) (c). Quantitative analysis of the atherosclerotic lesions in aortic sinuses of mice. The lesion area was measured as the percentage of aortic luminal area covered by atherosclerotic lesions. Values are given as mean ± standard deviation of the mean (n = 3). aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group (d).
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fig1: Intervention of FO+ALA-PS ameliorated atherosclerosis in HFD diet-fed mice. Photomicrographs of representative Oil-Red O stained aortas of mice in control, HFD, FO, and ALA-PS groups (a). Quantitative analysis of the atherosclerotic lesion aortas of mice. The lesion area was measured as the percentage of aortic luminal area covered by atherosclerotic lesions. Values are given as mean ± standard deviation of the mean (n = 3). aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group (b). Representative images of cross-sections taken of the aortic sinuses obtained from mice in control, HFD, FO, and ALA-PS groups. The sections of aortic sinuses were stained with Oil-Red O (magnification ×40) for lipid deposition (red) and cross-stained with hematoxylin (blue) (c). Quantitative analysis of the atherosclerotic lesions in aortic sinuses of mice. The lesion area was measured as the percentage of aortic luminal area covered by atherosclerotic lesions. Values are given as mean ± standard deviation of the mean (n = 3). aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group (d).

Mentions: En face analysis of aorta revealed atherosclerotic lesion formation with the aid of Oil-Red O staining. As representative results showed in Figure 1(a), compared with control, a significantly atherogenic action was induced in apoE-KO mice given HFD while the lesions in the FO and FO+ALA-PS groups were obviously decreased. Quantitative analysis of the atherosclerotic lesions in the aortas of mice (Figure 1(b)) demonstrated statistically smaller lesion size in FO+ALA-PS-treated animals than those in FO-treated mice (13.43 ± 2.75% versus 34.13 ± 10.00%).


Flaxseed Oil Containing α -Linolenic Acid Ester of Plant Sterol Improved Atherosclerosis in ApoE Deficient Mice.

Han H, Yan P, Chen L, Luo C, Gao H, Deng Q, Zheng M, Shi Y, Liu L - Oxid Med Cell Longev (2015)

Intervention of FO+ALA-PS ameliorated atherosclerosis in HFD diet-fed mice. Photomicrographs of representative Oil-Red O stained aortas of mice in control, HFD, FO, and ALA-PS groups (a). Quantitative analysis of the atherosclerotic lesion aortas of mice. The lesion area was measured as the percentage of aortic luminal area covered by atherosclerotic lesions. Values are given as mean ± standard deviation of the mean (n = 3). aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group (b). Representative images of cross-sections taken of the aortic sinuses obtained from mice in control, HFD, FO, and ALA-PS groups. The sections of aortic sinuses were stained with Oil-Red O (magnification ×40) for lipid deposition (red) and cross-stained with hematoxylin (blue) (c). Quantitative analysis of the atherosclerotic lesions in aortic sinuses of mice. The lesion area was measured as the percentage of aortic luminal area covered by atherosclerotic lesions. Values are given as mean ± standard deviation of the mean (n = 3). aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group (d).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4477243&req=5

fig1: Intervention of FO+ALA-PS ameliorated atherosclerosis in HFD diet-fed mice. Photomicrographs of representative Oil-Red O stained aortas of mice in control, HFD, FO, and ALA-PS groups (a). Quantitative analysis of the atherosclerotic lesion aortas of mice. The lesion area was measured as the percentage of aortic luminal area covered by atherosclerotic lesions. Values are given as mean ± standard deviation of the mean (n = 3). aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group (b). Representative images of cross-sections taken of the aortic sinuses obtained from mice in control, HFD, FO, and ALA-PS groups. The sections of aortic sinuses were stained with Oil-Red O (magnification ×40) for lipid deposition (red) and cross-stained with hematoxylin (blue) (c). Quantitative analysis of the atherosclerotic lesions in aortic sinuses of mice. The lesion area was measured as the percentage of aortic luminal area covered by atherosclerotic lesions. Values are given as mean ± standard deviation of the mean (n = 3). aP < 0.05 versus the control; bP < 0.05 versus the HFD group; cP < 0.05 versus the FO group (d).
Mentions: En face analysis of aorta revealed atherosclerotic lesion formation with the aid of Oil-Red O staining. As representative results showed in Figure 1(a), compared with control, a significantly atherogenic action was induced in apoE-KO mice given HFD while the lesions in the FO and FO+ALA-PS groups were obviously decreased. Quantitative analysis of the atherosclerotic lesions in the aortas of mice (Figure 1(b)) demonstrated statistically smaller lesion size in FO+ALA-PS-treated animals than those in FO-treated mice (13.43 ± 2.75% versus 34.13 ± 10.00%).

Bottom Line: Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties.Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress.These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

ABSTRACT
Plant sterols (PS) have potential preventive function in atherosclerosis due to their cholesterol-lowering ability. Dietary α-linolenic acid in flaxseed oil is associated with a reduction in cardiovascular events through its hypolipidemic and anti-inflammation properties. This study was designed to evaluate the effects of flaxseed oil containing α-linolenic acid ester of PS (ALA-PS) on atherosclerosis and investigate the underlying mechanisms. C57BL/6 mice were administered a regular diet and apoE knockout (apoE-KO) mice were given a high fat diet alone or supplemented with 5% flaxseed oil with or without 3.3% ALA-PS for 18 weeks. Results demonstrated that flaxseed oil containing ALA-PS was synergistically interaction in ameliorating atherosclerosis as well as optimizing overall lipid levels, inhibiting inflammation and reducing oxidative stress. These data were associated with the modification effects on expression levels of genes involved in lipid metabolism (PPARα, HMGCR, and SREBPs), inflammation (IL-6, TNF, MCP-1, and VCAM-1), and oxidative stress (NADPH oxidase).

No MeSH data available.


Related in: MedlinePlus