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Ionizing air affects influenza virus infectivity and prevents airborne-transmission.

Hagbom M, Nordgren J, Nybom R, Hedlund KO, Wigzell H, Svensson L - Sci Rep (2015)

Bottom Line: By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%).Active ionizer prevented 100% (4/4) of guinea pigs from infection.Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Virology, Department of Clinical and Experimental Medicine, University of Linköping, 581 85 Linköping, Sweden.

ABSTRACT
By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%). Active ionizer prevented 100% (4/4) of guinea pigs from infection. Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room. The ionizer generates negative ions, rendering airborne particles/aerosol droplets negatively charged and electrostatically attracts them to a positively charged collector plate. Trapped viruses are then identified by reverse transcription quantitative real-time PCR. The device enables unique possibilities for rapid and simple removal of virus from air and offers possibilities to simultaneously identify and prevent airborne transmission of viruses.

No MeSH data available.


Related in: MedlinePlus

Active ionizer prevents aerosol transmitted influenza virus (H3N2, Pan/99) infection between guinea pigs.While the active ionizer prevented 4 of 4 exposed guinea pigs from developing an immune response to influenza virus, 3 of 4 animals were infected when the inactive ionizer was used. Graph shows antibody titers by ELISA before infection (pre-serum 1, 2, 3 and 4) and at day 21 post-exposure to influenza virus (post-serum 1, 2, 3 and 4). Briefly, influenza virus H1N1; (SBL Influenza Vaccine, Sanofi Pasteur, Lyon, France) were coated on ELISA plates and incubated with two-fold dilutions of pre- and post- guinea pig sera, followed by biotinylated rabbit-anti-guinea pig antibody, HRP conjugated streptavidin and TMB substrate as described in Methods. Cut off (dashed line) value (0.284 OD) was the mean of the negative controls +2SD.
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f4: Active ionizer prevents aerosol transmitted influenza virus (H3N2, Pan/99) infection between guinea pigs.While the active ionizer prevented 4 of 4 exposed guinea pigs from developing an immune response to influenza virus, 3 of 4 animals were infected when the inactive ionizer was used. Graph shows antibody titers by ELISA before infection (pre-serum 1, 2, 3 and 4) and at day 21 post-exposure to influenza virus (post-serum 1, 2, 3 and 4). Briefly, influenza virus H1N1; (SBL Influenza Vaccine, Sanofi Pasteur, Lyon, France) were coated on ELISA plates and incubated with two-fold dilutions of pre- and post- guinea pig sera, followed by biotinylated rabbit-anti-guinea pig antibody, HRP conjugated streptavidin and TMB substrate as described in Methods. Cut off (dashed line) value (0.284 OD) was the mean of the negative controls +2SD.

Mentions: We assessed transmission of infection from animals in cage “A” to exposed uninfected animals in cage “B” by development of an immune response 21 days post exposure. The results shown in Fig. 4 illustrate that when the ionizer was inactive, 3 of the 4 uninfected but exposed animals developed a serum IgG influenza-specific immune responses. In contrast, none of the 4 animals in cage “B” developed an immune response to influenza virus when the ionizer was active (Fig. 4). Furthermore, influenza virus RNA could be detected by RT-qPCR, albeit at low concentration, on the collector plate from the active ionizer but not with the inactive ionizer, showing that the ionizing device indeed collected virus excreted from the infected animals in cage “A”.


Ionizing air affects influenza virus infectivity and prevents airborne-transmission.

Hagbom M, Nordgren J, Nybom R, Hedlund KO, Wigzell H, Svensson L - Sci Rep (2015)

Active ionizer prevents aerosol transmitted influenza virus (H3N2, Pan/99) infection between guinea pigs.While the active ionizer prevented 4 of 4 exposed guinea pigs from developing an immune response to influenza virus, 3 of 4 animals were infected when the inactive ionizer was used. Graph shows antibody titers by ELISA before infection (pre-serum 1, 2, 3 and 4) and at day 21 post-exposure to influenza virus (post-serum 1, 2, 3 and 4). Briefly, influenza virus H1N1; (SBL Influenza Vaccine, Sanofi Pasteur, Lyon, France) were coated on ELISA plates and incubated with two-fold dilutions of pre- and post- guinea pig sera, followed by biotinylated rabbit-anti-guinea pig antibody, HRP conjugated streptavidin and TMB substrate as described in Methods. Cut off (dashed line) value (0.284 OD) was the mean of the negative controls +2SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477231&req=5

f4: Active ionizer prevents aerosol transmitted influenza virus (H3N2, Pan/99) infection between guinea pigs.While the active ionizer prevented 4 of 4 exposed guinea pigs from developing an immune response to influenza virus, 3 of 4 animals were infected when the inactive ionizer was used. Graph shows antibody titers by ELISA before infection (pre-serum 1, 2, 3 and 4) and at day 21 post-exposure to influenza virus (post-serum 1, 2, 3 and 4). Briefly, influenza virus H1N1; (SBL Influenza Vaccine, Sanofi Pasteur, Lyon, France) were coated on ELISA plates and incubated with two-fold dilutions of pre- and post- guinea pig sera, followed by biotinylated rabbit-anti-guinea pig antibody, HRP conjugated streptavidin and TMB substrate as described in Methods. Cut off (dashed line) value (0.284 OD) was the mean of the negative controls +2SD.
Mentions: We assessed transmission of infection from animals in cage “A” to exposed uninfected animals in cage “B” by development of an immune response 21 days post exposure. The results shown in Fig. 4 illustrate that when the ionizer was inactive, 3 of the 4 uninfected but exposed animals developed a serum IgG influenza-specific immune responses. In contrast, none of the 4 animals in cage “B” developed an immune response to influenza virus when the ionizer was active (Fig. 4). Furthermore, influenza virus RNA could be detected by RT-qPCR, albeit at low concentration, on the collector plate from the active ionizer but not with the inactive ionizer, showing that the ionizing device indeed collected virus excreted from the infected animals in cage “A”.

Bottom Line: By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%).Active ionizer prevented 100% (4/4) of guinea pigs from infection.Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Virology, Department of Clinical and Experimental Medicine, University of Linköping, 581 85 Linköping, Sweden.

ABSTRACT
By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%). Active ionizer prevented 100% (4/4) of guinea pigs from infection. Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room. The ionizer generates negative ions, rendering airborne particles/aerosol droplets negatively charged and electrostatically attracts them to a positively charged collector plate. Trapped viruses are then identified by reverse transcription quantitative real-time PCR. The device enables unique possibilities for rapid and simple removal of virus from air and offers possibilities to simultaneously identify and prevent airborne transmission of viruses.

No MeSH data available.


Related in: MedlinePlus