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Ionizing air affects influenza virus infectivity and prevents airborne-transmission.

Hagbom M, Nordgren J, Nybom R, Hedlund KO, Wigzell H, Svensson L - Sci Rep (2015)

Bottom Line: By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%).Active ionizer prevented 100% (4/4) of guinea pigs from infection.Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Virology, Department of Clinical and Experimental Medicine, University of Linköping, 581 85 Linköping, Sweden.

ABSTRACT
By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%). Active ionizer prevented 100% (4/4) of guinea pigs from infection. Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room. The ionizer generates negative ions, rendering airborne particles/aerosol droplets negatively charged and electrostatically attracts them to a positively charged collector plate. Trapped viruses are then identified by reverse transcription quantitative real-time PCR. The device enables unique possibilities for rapid and simple removal of virus from air and offers possibilities to simultaneously identify and prevent airborne transmission of viruses.

No MeSH data available.


Related in: MedlinePlus

Set-up design of influenza virus (H3N2, Pan/99) aerosol-transmission experiments between guinea pigs. Guinea pigs (n = 4) were intranasally infected with 5 × 103 pfu of Pan/99 virus in 100 uL (50 uL in each nostril).All four infected animals were placed into an experimental cage “A”. At 30 h p.i. four naïve uninfected guinea pigs were placed in cage “B” . Air-flow from left to right. Air exchanged 17x/day. Filled rectangle = ionizer.
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f3: Set-up design of influenza virus (H3N2, Pan/99) aerosol-transmission experiments between guinea pigs. Guinea pigs (n = 4) were intranasally infected with 5 × 103 pfu of Pan/99 virus in 100 uL (50 uL in each nostril).All four infected animals were placed into an experimental cage “A”. At 30 h p.i. four naïve uninfected guinea pigs were placed in cage “B” . Air-flow from left to right. Air exchanged 17x/day. Filled rectangle = ionizer.

Mentions: Next we took advantage of an established influenza guinea pig model313233 to study if ionizing air and electrostatic attraction could prevent airborne aerosol and droplet transmitted influenza A/Panama (Pan/99) virus infection between guinea pigs. The airborne/droplet transmission model was established essentially as described31 using two separate cages with the ionizer placed between the cages (Fig. 3). Four guinea pigs were infected by intranasal route as described with 5 ×103 pfu of Pan/9931 and placed in cage “A” (Fig. 3). At 30 hours post infection (h p.i.) 4 uninfected guinea pigs were placed in cage “B” 15 cm from the cage with infected animals as illustrated in Fig. 3, with no physical contact. The ionizer was placed between cages “A” and “B”. Two identical experiments were performed, one with active ionizer placed between the cages and one with an inactive ionizer.


Ionizing air affects influenza virus infectivity and prevents airborne-transmission.

Hagbom M, Nordgren J, Nybom R, Hedlund KO, Wigzell H, Svensson L - Sci Rep (2015)

Set-up design of influenza virus (H3N2, Pan/99) aerosol-transmission experiments between guinea pigs. Guinea pigs (n = 4) were intranasally infected with 5 × 103 pfu of Pan/99 virus in 100 uL (50 uL in each nostril).All four infected animals were placed into an experimental cage “A”. At 30 h p.i. four naïve uninfected guinea pigs were placed in cage “B” . Air-flow from left to right. Air exchanged 17x/day. Filled rectangle = ionizer.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477231&req=5

f3: Set-up design of influenza virus (H3N2, Pan/99) aerosol-transmission experiments between guinea pigs. Guinea pigs (n = 4) were intranasally infected with 5 × 103 pfu of Pan/99 virus in 100 uL (50 uL in each nostril).All four infected animals were placed into an experimental cage “A”. At 30 h p.i. four naïve uninfected guinea pigs were placed in cage “B” . Air-flow from left to right. Air exchanged 17x/day. Filled rectangle = ionizer.
Mentions: Next we took advantage of an established influenza guinea pig model313233 to study if ionizing air and electrostatic attraction could prevent airborne aerosol and droplet transmitted influenza A/Panama (Pan/99) virus infection between guinea pigs. The airborne/droplet transmission model was established essentially as described31 using two separate cages with the ionizer placed between the cages (Fig. 3). Four guinea pigs were infected by intranasal route as described with 5 ×103 pfu of Pan/9931 and placed in cage “A” (Fig. 3). At 30 hours post infection (h p.i.) 4 uninfected guinea pigs were placed in cage “B” 15 cm from the cage with infected animals as illustrated in Fig. 3, with no physical contact. The ionizer was placed between cages “A” and “B”. Two identical experiments were performed, one with active ionizer placed between the cages and one with an inactive ionizer.

Bottom Line: By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%).Active ionizer prevented 100% (4/4) of guinea pigs from infection.Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Virology, Department of Clinical and Experimental Medicine, University of Linköping, 581 85 Linköping, Sweden.

ABSTRACT
By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%). Active ionizer prevented 100% (4/4) of guinea pigs from infection. Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room. The ionizer generates negative ions, rendering airborne particles/aerosol droplets negatively charged and electrostatically attracts them to a positively charged collector plate. Trapped viruses are then identified by reverse transcription quantitative real-time PCR. The device enables unique possibilities for rapid and simple removal of virus from air and offers possibilities to simultaneously identify and prevent airborne transmission of viruses.

No MeSH data available.


Related in: MedlinePlus