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Ionizing air affects influenza virus infectivity and prevents airborne-transmission.

Hagbom M, Nordgren J, Nybom R, Hedlund KO, Wigzell H, Svensson L - Sci Rep (2015)

Bottom Line: By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%).Active ionizer prevented 100% (4/4) of guinea pigs from infection.Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Virology, Department of Clinical and Experimental Medicine, University of Linköping, 581 85 Linköping, Sweden.

ABSTRACT
By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%). Active ionizer prevented 100% (4/4) of guinea pigs from infection. Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room. The ionizer generates negative ions, rendering airborne particles/aerosol droplets negatively charged and electrostatically attracts them to a positively charged collector plate. Trapped viruses are then identified by reverse transcription quantitative real-time PCR. The device enables unique possibilities for rapid and simple removal of virus from air and offers possibilities to simultaneously identify and prevent airborne transmission of viruses.

No MeSH data available.


Related in: MedlinePlus

Real-time PCR on trapped rotavirus (a), calicivirus (b) and influenza virus (H1N1; strain Salomon Island) (c). Note that no influenza virus was detected on the inactive ionizer.
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f2: Real-time PCR on trapped rotavirus (a), calicivirus (b) and influenza virus (H1N1; strain Salomon Island) (c). Note that no influenza virus was detected on the inactive ionizer.

Mentions: We next determined the capacity of RT-qPCR technology to quantitate the capacity of the ionizer technique to collect and concentrate viruses. Three independent experiments with each of the three viruses were carried out using the same virus concentrations in each experiment (Fig. 2a–c). Although several steps are involved from collection to detection the system was robust as to reproducibility. The RT-qPCR data shows that the active collector is concentrating and collecting virus 1500–3000 times more efficient as compared to the inactive collector (Table 1). When different dilutions of virus was used for aerosol production the proportion of aerosolized virus collected on the active collector was normally in the range of 0.1–0.6% for CaCV, rotavirus and influenza virus. A reproducible finding with regard to CaCV was a significant increase in relative recovery at the lowest concentrations increasing to 10–20% of the total amount of virus aerosolized (Table 1).


Ionizing air affects influenza virus infectivity and prevents airborne-transmission.

Hagbom M, Nordgren J, Nybom R, Hedlund KO, Wigzell H, Svensson L - Sci Rep (2015)

Real-time PCR on trapped rotavirus (a), calicivirus (b) and influenza virus (H1N1; strain Salomon Island) (c). Note that no influenza virus was detected on the inactive ionizer.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477231&req=5

f2: Real-time PCR on trapped rotavirus (a), calicivirus (b) and influenza virus (H1N1; strain Salomon Island) (c). Note that no influenza virus was detected on the inactive ionizer.
Mentions: We next determined the capacity of RT-qPCR technology to quantitate the capacity of the ionizer technique to collect and concentrate viruses. Three independent experiments with each of the three viruses were carried out using the same virus concentrations in each experiment (Fig. 2a–c). Although several steps are involved from collection to detection the system was robust as to reproducibility. The RT-qPCR data shows that the active collector is concentrating and collecting virus 1500–3000 times more efficient as compared to the inactive collector (Table 1). When different dilutions of virus was used for aerosol production the proportion of aerosolized virus collected on the active collector was normally in the range of 0.1–0.6% for CaCV, rotavirus and influenza virus. A reproducible finding with regard to CaCV was a significant increase in relative recovery at the lowest concentrations increasing to 10–20% of the total amount of virus aerosolized (Table 1).

Bottom Line: By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%).Active ionizer prevented 100% (4/4) of guinea pigs from infection.Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Virology, Department of Clinical and Experimental Medicine, University of Linköping, 581 85 Linköping, Sweden.

ABSTRACT
By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%). Active ionizer prevented 100% (4/4) of guinea pigs from infection. Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room. The ionizer generates negative ions, rendering airborne particles/aerosol droplets negatively charged and electrostatically attracts them to a positively charged collector plate. Trapped viruses are then identified by reverse transcription quantitative real-time PCR. The device enables unique possibilities for rapid and simple removal of virus from air and offers possibilities to simultaneously identify and prevent airborne transmission of viruses.

No MeSH data available.


Related in: MedlinePlus