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Ionizing air affects influenza virus infectivity and prevents airborne-transmission.

Hagbom M, Nordgren J, Nybom R, Hedlund KO, Wigzell H, Svensson L - Sci Rep (2015)

Bottom Line: By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%).Active ionizer prevented 100% (4/4) of guinea pigs from infection.Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Virology, Department of Clinical and Experimental Medicine, University of Linköping, 581 85 Linköping, Sweden.

ABSTRACT
By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%). Active ionizer prevented 100% (4/4) of guinea pigs from infection. Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room. The ionizer generates negative ions, rendering airborne particles/aerosol droplets negatively charged and electrostatically attracts them to a positively charged collector plate. Trapped viruses are then identified by reverse transcription quantitative real-time PCR. The device enables unique possibilities for rapid and simple removal of virus from air and offers possibilities to simultaneously identify and prevent airborne transmission of viruses.

No MeSH data available.


Related in: MedlinePlus

Airpoint ionizer with collector plate (size 13 × 35 cm) (a). The ionizing device was developed based of the Ion-Flow Ionizing Technology from LightAir AB, Solna, Sweden and was modified by installing a plastic-cup with a conductive surface of 47 mm in diameter, with positive charge, as the collector plate; Aerosolized and trapped latex particles (>1 to <10 μm) on active (b) and inactive (c) ionizer, (bar = 10 μM); Rotavirus (d); and influenza virus (H1N1; strain Salomon Island) (e) trapped on active ionizer, (Bar = 50 nm).
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f1: Airpoint ionizer with collector plate (size 13 × 35 cm) (a). The ionizing device was developed based of the Ion-Flow Ionizing Technology from LightAir AB, Solna, Sweden and was modified by installing a plastic-cup with a conductive surface of 47 mm in diameter, with positive charge, as the collector plate; Aerosolized and trapped latex particles (>1 to <10 μm) on active (b) and inactive (c) ionizer, (bar = 10 μM); Rotavirus (d); and influenza virus (H1N1; strain Salomon Island) (e) trapped on active ionizer, (Bar = 50 nm).

Mentions: The device (Fig. 1a) consists of a small portable 12 volt operated ionizer, with a collector plate of positive charge attached to the ionizer, attracting negative particles from the air by electrostatic attraction. To determine optimal time collection parameters, latex particles with sizes ranging from <1 to >10 μm were nebulized into a room of 19 m3. Testing revealed that 40–60 min was required to eliminate >90% of free latex particles in the air as determined by real-time particle counting (PortaCount Plus). The particle counter can detect particles with size greater than 0.02 μM. Visualization by scanning electron microscopy (SEM) on grids from active- and inactive ionizer collector plates showed that accumulation of latex particles was dramatically enhanced on active ionizer collector plates compared to the inactive (Fig. 1b,c). Next, high numbers of rotavirus and formalin-inactivated influenza virus were aerosolized under the same conditions. While, after 40 min the inactive collector plates contained few (<5) rotavirus and influenza virus, the active collector contained >50 virus particles, as determined by transmission electron microscopy (TEM), (Fig. 1d,e).


Ionizing air affects influenza virus infectivity and prevents airborne-transmission.

Hagbom M, Nordgren J, Nybom R, Hedlund KO, Wigzell H, Svensson L - Sci Rep (2015)

Airpoint ionizer with collector plate (size 13 × 35 cm) (a). The ionizing device was developed based of the Ion-Flow Ionizing Technology from LightAir AB, Solna, Sweden and was modified by installing a plastic-cup with a conductive surface of 47 mm in diameter, with positive charge, as the collector plate; Aerosolized and trapped latex particles (>1 to <10 μm) on active (b) and inactive (c) ionizer, (bar = 10 μM); Rotavirus (d); and influenza virus (H1N1; strain Salomon Island) (e) trapped on active ionizer, (Bar = 50 nm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477231&req=5

f1: Airpoint ionizer with collector plate (size 13 × 35 cm) (a). The ionizing device was developed based of the Ion-Flow Ionizing Technology from LightAir AB, Solna, Sweden and was modified by installing a plastic-cup with a conductive surface of 47 mm in diameter, with positive charge, as the collector plate; Aerosolized and trapped latex particles (>1 to <10 μm) on active (b) and inactive (c) ionizer, (bar = 10 μM); Rotavirus (d); and influenza virus (H1N1; strain Salomon Island) (e) trapped on active ionizer, (Bar = 50 nm).
Mentions: The device (Fig. 1a) consists of a small portable 12 volt operated ionizer, with a collector plate of positive charge attached to the ionizer, attracting negative particles from the air by electrostatic attraction. To determine optimal time collection parameters, latex particles with sizes ranging from <1 to >10 μm were nebulized into a room of 19 m3. Testing revealed that 40–60 min was required to eliminate >90% of free latex particles in the air as determined by real-time particle counting (PortaCount Plus). The particle counter can detect particles with size greater than 0.02 μM. Visualization by scanning electron microscopy (SEM) on grids from active- and inactive ionizer collector plates showed that accumulation of latex particles was dramatically enhanced on active ionizer collector plates compared to the inactive (Fig. 1b,c). Next, high numbers of rotavirus and formalin-inactivated influenza virus were aerosolized under the same conditions. While, after 40 min the inactive collector plates contained few (<5) rotavirus and influenza virus, the active collector contained >50 virus particles, as determined by transmission electron microscopy (TEM), (Fig. 1d,e).

Bottom Line: By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%).Active ionizer prevented 100% (4/4) of guinea pigs from infection.Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Virology, Department of Clinical and Experimental Medicine, University of Linköping, 581 85 Linköping, Sweden.

ABSTRACT
By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%). Active ionizer prevented 100% (4/4) of guinea pigs from infection. Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m(3) room. The ionizer generates negative ions, rendering airborne particles/aerosol droplets negatively charged and electrostatically attracts them to a positively charged collector plate. Trapped viruses are then identified by reverse transcription quantitative real-time PCR. The device enables unique possibilities for rapid and simple removal of virus from air and offers possibilities to simultaneously identify and prevent airborne transmission of viruses.

No MeSH data available.


Related in: MedlinePlus