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Effect of papain-based gel on type I collagen--spectroscopy applied for microstructural analysis.

Silva Júnior ZS, Botta SB, Ana PA, França CM, Fernandes KP, Mesquita-Ferrari RA, Deana A, Bussadori SK - Sci Rep (2015)

Bottom Line: In a further experiment, 10 samples of type I collagen membrane were used to evaluate the effects of Papacarie(TM) on the collagen microstructure.The results demonstrated that the application of Papacarie(TM) does not lead to the degradation of collagen and this product can be safely used in minimally invasive dentistry.As the integrity of sound collagen is preserved after the application of the papain-based gel, this product is indicated for the selective removal of infected dentin, leaving the affected dentin intact and capable of re-mineralization.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.

ABSTRACT
Considering the improvement of biomaterials that facilitate atraumatic restorative techniques in dentistry, a papain-based gel can be used in the chemomechanical removal of decayed dental tissue. However, there is no information regarding the influence of this gel on the structure of sound collagen. The aim of the present study was to investigate the adsorption of a papain-based gel (Papacarie(TM)) to collagen and determine collagen integrity after treatment. A pilot study was first performed with 10 samples of type I collagen membrane obtained from bovine Achilles deep tendon to compare the influence of hydration (Milli-Q water) on infrared bands of collagen. In a further experiment, 10 samples of type I collagen membrane were used to evaluate the effects of Papacarie(TM) on the collagen microstructure. All analyses were performed using the attenuated total reflectance technique of Fourier transform infrared (ATR-FTIR). The results demonstrated that the application of Papacarie(TM) does not lead to the degradation of collagen and this product can be safely used in minimally invasive dentistry. As the integrity of sound collagen is preserved after the application of the papain-based gel, this product is indicated for the selective removal of infected dentin, leaving the affected dentin intact and capable of re-mineralization.

No MeSH data available.


Mean infrared type I collagen spectrum obtained for dry membranes and membranes washed with Milli-Q water (hydrated) in region from 3800 to 800 cm−1.
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f3: Mean infrared type I collagen spectrum obtained for dry membranes and membranes washed with Milli-Q water (hydrated) in region from 3800 to 800 cm−1.

Mentions: Figure 3 displays the results of the pilot study. The exposure of the collagen membranes to Milli-Q water significantly increased the infrared band from 3000 to 3500 cm−1, which corresponds to asymmetric and symmetric O-H stretching. Moreover, an increase occurred in the intensity of all infrared bands in the fingerprint region in comparison to the dry collagen membranes.


Effect of papain-based gel on type I collagen--spectroscopy applied for microstructural analysis.

Silva Júnior ZS, Botta SB, Ana PA, França CM, Fernandes KP, Mesquita-Ferrari RA, Deana A, Bussadori SK - Sci Rep (2015)

Mean infrared type I collagen spectrum obtained for dry membranes and membranes washed with Milli-Q water (hydrated) in region from 3800 to 800 cm−1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477230&req=5

f3: Mean infrared type I collagen spectrum obtained for dry membranes and membranes washed with Milli-Q water (hydrated) in region from 3800 to 800 cm−1.
Mentions: Figure 3 displays the results of the pilot study. The exposure of the collagen membranes to Milli-Q water significantly increased the infrared band from 3000 to 3500 cm−1, which corresponds to asymmetric and symmetric O-H stretching. Moreover, an increase occurred in the intensity of all infrared bands in the fingerprint region in comparison to the dry collagen membranes.

Bottom Line: In a further experiment, 10 samples of type I collagen membrane were used to evaluate the effects of Papacarie(TM) on the collagen microstructure.The results demonstrated that the application of Papacarie(TM) does not lead to the degradation of collagen and this product can be safely used in minimally invasive dentistry.As the integrity of sound collagen is preserved after the application of the papain-based gel, this product is indicated for the selective removal of infected dentin, leaving the affected dentin intact and capable of re-mineralization.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.

ABSTRACT
Considering the improvement of biomaterials that facilitate atraumatic restorative techniques in dentistry, a papain-based gel can be used in the chemomechanical removal of decayed dental tissue. However, there is no information regarding the influence of this gel on the structure of sound collagen. The aim of the present study was to investigate the adsorption of a papain-based gel (Papacarie(TM)) to collagen and determine collagen integrity after treatment. A pilot study was first performed with 10 samples of type I collagen membrane obtained from bovine Achilles deep tendon to compare the influence of hydration (Milli-Q water) on infrared bands of collagen. In a further experiment, 10 samples of type I collagen membrane were used to evaluate the effects of Papacarie(TM) on the collagen microstructure. All analyses were performed using the attenuated total reflectance technique of Fourier transform infrared (ATR-FTIR). The results demonstrated that the application of Papacarie(TM) does not lead to the degradation of collagen and this product can be safely used in minimally invasive dentistry. As the integrity of sound collagen is preserved after the application of the papain-based gel, this product is indicated for the selective removal of infected dentin, leaving the affected dentin intact and capable of re-mineralization.

No MeSH data available.