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Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer's Disease.

Masci A, Mattioli R, Costantino P, Baima S, Morelli G, Punzi P, Giordano C, Pinto A, Donini LM, d'Erme M, Mosca L - Oxid Med Cell Longev (2015)

Bottom Line: In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions.Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2).Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine-Medical Physiopathology, Food Science and Endocrinology Section, Sapienza University, Rome, Italy.

ABSTRACT

Unlabelled: β-Amyloid peptide (Aβ) aberrant production and aggregation are major factors implicated in the pathogenesis of Alzheimer's disease (AD), causing neuronal death via oxidative stress. Several studies have highlighted the importance of polyphenolic antioxidant compounds in the treatment of AD, but complex food matrices, characterized by a different relative content of these phytochemicals, have been neglected. In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions. Both juices protected against Aβ-induced cytotoxicity and apoptotic cell death as evidenced by cell viability, nuclear chromatin condensation, and apoptotic body formation measurements. These effects were mediated by the modulation of the mitochondrial function and of the HSP70 gene transcription and expression. Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and

Nad(p)h: quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2). Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

No MeSH data available.


Related in: MedlinePlus

Broccoli sprouts juices activate nuclear translocation of Nrf2 transcription factor which controls the expression of cell survival genes involved in defence against oxidative stress-mediated damage. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 3 h. (a) Nrf2 nuclear translocation was verified by immunofluorescence microscopy by utilizing anti-Nrf2 antibody and DAPI staining. Representative images of three independent experiments show the stimulation of Nrf2 nuclear accumulation by broccoli sprouts juices A or B exposure, compared to both control cells and Aβ25–35 alone treated cells. (b) Densitometric analysis of the mean nuclear located-Nrf2 fluorescence signal as calculated analyzing immunofluorescence images by the Image J software. Data are expressed as percentage of control and represented as mean ± SEM (n = 3), aP < 0.001 versus control; bP < 0.001 versus Aβ25–35; cP < 0.001 versus juice A; dP < 0.001 versus (A + Aβ).
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fig7: Broccoli sprouts juices activate nuclear translocation of Nrf2 transcription factor which controls the expression of cell survival genes involved in defence against oxidative stress-mediated damage. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 3 h. (a) Nrf2 nuclear translocation was verified by immunofluorescence microscopy by utilizing anti-Nrf2 antibody and DAPI staining. Representative images of three independent experiments show the stimulation of Nrf2 nuclear accumulation by broccoli sprouts juices A or B exposure, compared to both control cells and Aβ25–35 alone treated cells. (b) Densitometric analysis of the mean nuclear located-Nrf2 fluorescence signal as calculated analyzing immunofluorescence images by the Image J software. Data are expressed as percentage of control and represented as mean ± SEM (n = 3), aP < 0.001 versus control; bP < 0.001 versus Aβ25–35; cP < 0.001 versus juice A; dP < 0.001 versus (A + Aβ).

Mentions: To elucidate the upstream signalling pathway involved in the broccoli sprouts juices-induced upregulation of the antioxidant enzymes, we focused on the activation of the redox-sensitive transcription factor Nrf2. When SH-SY5Y cells were treated with Aβ25–35, or with juices A or B, alone or in the presence of Aβ25–35, differences in Nrf2 nuclear translocation between samples after 3 h of incubation were observed (Figures 7(a) and 7(b)). In particular, densitometric analysis of the images collected by immunofluorescence microscopy showed that, compared to control cells, the nuclear content of Nrf2 was not altered by Aβ25–35, while both broccoli sprouts juices were able to induce nuclear translocation of Nrf2, both alone (157% A versus control, P < 0.001; 246% B versus control, P < 0.001) and in the presence of Aβ25–35 (152% (A + Aβ) versus control and Aβ25–35, P < 0.001; 291% (B + Aβ) versus control and Aβ25–35, P < 0.001), with juice B being significantly more effective than juice A.


Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer's Disease.

Masci A, Mattioli R, Costantino P, Baima S, Morelli G, Punzi P, Giordano C, Pinto A, Donini LM, d'Erme M, Mosca L - Oxid Med Cell Longev (2015)

Broccoli sprouts juices activate nuclear translocation of Nrf2 transcription factor which controls the expression of cell survival genes involved in defence against oxidative stress-mediated damage. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 3 h. (a) Nrf2 nuclear translocation was verified by immunofluorescence microscopy by utilizing anti-Nrf2 antibody and DAPI staining. Representative images of three independent experiments show the stimulation of Nrf2 nuclear accumulation by broccoli sprouts juices A or B exposure, compared to both control cells and Aβ25–35 alone treated cells. (b) Densitometric analysis of the mean nuclear located-Nrf2 fluorescence signal as calculated analyzing immunofluorescence images by the Image J software. Data are expressed as percentage of control and represented as mean ± SEM (n = 3), aP < 0.001 versus control; bP < 0.001 versus Aβ25–35; cP < 0.001 versus juice A; dP < 0.001 versus (A + Aβ).
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig7: Broccoli sprouts juices activate nuclear translocation of Nrf2 transcription factor which controls the expression of cell survival genes involved in defence against oxidative stress-mediated damage. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 3 h. (a) Nrf2 nuclear translocation was verified by immunofluorescence microscopy by utilizing anti-Nrf2 antibody and DAPI staining. Representative images of three independent experiments show the stimulation of Nrf2 nuclear accumulation by broccoli sprouts juices A or B exposure, compared to both control cells and Aβ25–35 alone treated cells. (b) Densitometric analysis of the mean nuclear located-Nrf2 fluorescence signal as calculated analyzing immunofluorescence images by the Image J software. Data are expressed as percentage of control and represented as mean ± SEM (n = 3), aP < 0.001 versus control; bP < 0.001 versus Aβ25–35; cP < 0.001 versus juice A; dP < 0.001 versus (A + Aβ).
Mentions: To elucidate the upstream signalling pathway involved in the broccoli sprouts juices-induced upregulation of the antioxidant enzymes, we focused on the activation of the redox-sensitive transcription factor Nrf2. When SH-SY5Y cells were treated with Aβ25–35, or with juices A or B, alone or in the presence of Aβ25–35, differences in Nrf2 nuclear translocation between samples after 3 h of incubation were observed (Figures 7(a) and 7(b)). In particular, densitometric analysis of the images collected by immunofluorescence microscopy showed that, compared to control cells, the nuclear content of Nrf2 was not altered by Aβ25–35, while both broccoli sprouts juices were able to induce nuclear translocation of Nrf2, both alone (157% A versus control, P < 0.001; 246% B versus control, P < 0.001) and in the presence of Aβ25–35 (152% (A + Aβ) versus control and Aβ25–35, P < 0.001; 291% (B + Aβ) versus control and Aβ25–35, P < 0.001), with juice B being significantly more effective than juice A.

Bottom Line: In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions.Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2).Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine-Medical Physiopathology, Food Science and Endocrinology Section, Sapienza University, Rome, Italy.

ABSTRACT

Unlabelled: β-Amyloid peptide (Aβ) aberrant production and aggregation are major factors implicated in the pathogenesis of Alzheimer's disease (AD), causing neuronal death via oxidative stress. Several studies have highlighted the importance of polyphenolic antioxidant compounds in the treatment of AD, but complex food matrices, characterized by a different relative content of these phytochemicals, have been neglected. In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions. Both juices protected against Aβ-induced cytotoxicity and apoptotic cell death as evidenced by cell viability, nuclear chromatin condensation, and apoptotic body formation measurements. These effects were mediated by the modulation of the mitochondrial function and of the HSP70 gene transcription and expression. Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and

Nad(p)h: quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2). Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

No MeSH data available.


Related in: MedlinePlus