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Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer's Disease.

Masci A, Mattioli R, Costantino P, Baima S, Morelli G, Punzi P, Giordano C, Pinto A, Donini LM, d'Erme M, Mosca L - Oxid Med Cell Longev (2015)

Bottom Line: In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions.Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2).Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine-Medical Physiopathology, Food Science and Endocrinology Section, Sapienza University, Rome, Italy.

ABSTRACT

Unlabelled: β-Amyloid peptide (Aβ) aberrant production and aggregation are major factors implicated in the pathogenesis of Alzheimer's disease (AD), causing neuronal death via oxidative stress. Several studies have highlighted the importance of polyphenolic antioxidant compounds in the treatment of AD, but complex food matrices, characterized by a different relative content of these phytochemicals, have been neglected. In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions. Both juices protected against Aβ-induced cytotoxicity and apoptotic cell death as evidenced by cell viability, nuclear chromatin condensation, and apoptotic body formation measurements. These effects were mediated by the modulation of the mitochondrial function and of the HSP70 gene transcription and expression. Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and

Nad(p)h: quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2). Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

No MeSH data available.


Related in: MedlinePlus

Effect of broccoli sprouts juices on Aβ25–35-induced mitochondrial membrane potential (ΔΨm) depletion. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 1, 4, 7, 24, 48, and 72 h. After treatment the cells were stained with JC-1 and analyzed by flow cytometry. The ΔΨm is proportional to the ratio between red and green fluorescence of the mitochondrial probe JC-1 and is expressed as a percentage of the control. Data are represented as mean ± SEM (n = 3), aP < 0.05, bP < 0.01, and cP < 0.001 versus control; dP < 0.001 versus Aβ25–35; eP < 0.05 versus juice A; fP < 0.05 versus (A + Aβ).
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fig3: Effect of broccoli sprouts juices on Aβ25–35-induced mitochondrial membrane potential (ΔΨm) depletion. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 1, 4, 7, 24, 48, and 72 h. After treatment the cells were stained with JC-1 and analyzed by flow cytometry. The ΔΨm is proportional to the ratio between red and green fluorescence of the mitochondrial probe JC-1 and is expressed as a percentage of the control. Data are represented as mean ± SEM (n = 3), aP < 0.05, bP < 0.01, and cP < 0.001 versus control; dP < 0.001 versus Aβ25–35; eP < 0.05 versus juice A; fP < 0.05 versus (A + Aβ).

Mentions: We also confirmed the protective effect of broccoli sprouts juices against Aβ25–35-induced apoptotic cell death by examining a proapoptotic signal such as the reduction of the ΔΨm. Cells exposed to 25 μM Aβ25–35 showed a rapid reduction in ΔΨm (Figure 3), which at 1 and 4 h of incubation was found to be ~20% compared to control cells (P < 0.01), whereas starting from 7 h up to 72 h it was ~30% (statistical significance ranging from P < 0.05 to P < 0.001). During the first 7 h of treatment with 10 μL/mL of juice A, no significant changes in ΔΨm were observed whereas a marked and progressive increase for longer exposure times was evidenced, with ΔΨm values reaching 205% of control at 48 h (P < 0.01) and 194% of control at 72 h (P < 0.05). When the cells were incubated with 10 μL/mL of juice B, a decrease of ΔΨm (73% versus control, P < 0.05) was observed after 4 h of exposure, but on the other hand the ΔΨm readily returned to control values within 7 h of treatment. Similarly to what was observed for juice A, also juice B induced a significant increase of ΔΨm, from 48 h of exposure (195%, P < 0.05) and up to 72 h (217%, P < 0.01), compared to control cells. When the cells were treated with Aβ25–35 in the presence of juice A or juice B, no protective effect on the ΔΨm was observed within 48 h of treatment, whereas after 72 h of incubation, broccoli sprouts juices significantly improved Aβ25–35-induced impairment in ΔΨm, with juice B being more effective than juice A (75% versus 56%, resp., P < 0.05).


Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer's Disease.

Masci A, Mattioli R, Costantino P, Baima S, Morelli G, Punzi P, Giordano C, Pinto A, Donini LM, d'Erme M, Mosca L - Oxid Med Cell Longev (2015)

Effect of broccoli sprouts juices on Aβ25–35-induced mitochondrial membrane potential (ΔΨm) depletion. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 1, 4, 7, 24, 48, and 72 h. After treatment the cells were stained with JC-1 and analyzed by flow cytometry. The ΔΨm is proportional to the ratio between red and green fluorescence of the mitochondrial probe JC-1 and is expressed as a percentage of the control. Data are represented as mean ± SEM (n = 3), aP < 0.05, bP < 0.01, and cP < 0.001 versus control; dP < 0.001 versus Aβ25–35; eP < 0.05 versus juice A; fP < 0.05 versus (A + Aβ).
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: Effect of broccoli sprouts juices on Aβ25–35-induced mitochondrial membrane potential (ΔΨm) depletion. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 1, 4, 7, 24, 48, and 72 h. After treatment the cells were stained with JC-1 and analyzed by flow cytometry. The ΔΨm is proportional to the ratio between red and green fluorescence of the mitochondrial probe JC-1 and is expressed as a percentage of the control. Data are represented as mean ± SEM (n = 3), aP < 0.05, bP < 0.01, and cP < 0.001 versus control; dP < 0.001 versus Aβ25–35; eP < 0.05 versus juice A; fP < 0.05 versus (A + Aβ).
Mentions: We also confirmed the protective effect of broccoli sprouts juices against Aβ25–35-induced apoptotic cell death by examining a proapoptotic signal such as the reduction of the ΔΨm. Cells exposed to 25 μM Aβ25–35 showed a rapid reduction in ΔΨm (Figure 3), which at 1 and 4 h of incubation was found to be ~20% compared to control cells (P < 0.01), whereas starting from 7 h up to 72 h it was ~30% (statistical significance ranging from P < 0.05 to P < 0.001). During the first 7 h of treatment with 10 μL/mL of juice A, no significant changes in ΔΨm were observed whereas a marked and progressive increase for longer exposure times was evidenced, with ΔΨm values reaching 205% of control at 48 h (P < 0.01) and 194% of control at 72 h (P < 0.05). When the cells were incubated with 10 μL/mL of juice B, a decrease of ΔΨm (73% versus control, P < 0.05) was observed after 4 h of exposure, but on the other hand the ΔΨm readily returned to control values within 7 h of treatment. Similarly to what was observed for juice A, also juice B induced a significant increase of ΔΨm, from 48 h of exposure (195%, P < 0.05) and up to 72 h (217%, P < 0.01), compared to control cells. When the cells were treated with Aβ25–35 in the presence of juice A or juice B, no protective effect on the ΔΨm was observed within 48 h of treatment, whereas after 72 h of incubation, broccoli sprouts juices significantly improved Aβ25–35-induced impairment in ΔΨm, with juice B being more effective than juice A (75% versus 56%, resp., P < 0.05).

Bottom Line: In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions.Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2).Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine-Medical Physiopathology, Food Science and Endocrinology Section, Sapienza University, Rome, Italy.

ABSTRACT

Unlabelled: β-Amyloid peptide (Aβ) aberrant production and aggregation are major factors implicated in the pathogenesis of Alzheimer's disease (AD), causing neuronal death via oxidative stress. Several studies have highlighted the importance of polyphenolic antioxidant compounds in the treatment of AD, but complex food matrices, characterized by a different relative content of these phytochemicals, have been neglected. In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions. Both juices protected against Aβ-induced cytotoxicity and apoptotic cell death as evidenced by cell viability, nuclear chromatin condensation, and apoptotic body formation measurements. These effects were mediated by the modulation of the mitochondrial function and of the HSP70 gene transcription and expression. Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and

Nad(p)h: quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2). Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

No MeSH data available.


Related in: MedlinePlus