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Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer's Disease.

Masci A, Mattioli R, Costantino P, Baima S, Morelli G, Punzi P, Giordano C, Pinto A, Donini LM, d'Erme M, Mosca L - Oxid Med Cell Longev (2015)

Bottom Line: In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions.Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2).Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine-Medical Physiopathology, Food Science and Endocrinology Section, Sapienza University, Rome, Italy.

ABSTRACT

Unlabelled: β-Amyloid peptide (Aβ) aberrant production and aggregation are major factors implicated in the pathogenesis of Alzheimer's disease (AD), causing neuronal death via oxidative stress. Several studies have highlighted the importance of polyphenolic antioxidant compounds in the treatment of AD, but complex food matrices, characterized by a different relative content of these phytochemicals, have been neglected. In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions. Both juices protected against Aβ-induced cytotoxicity and apoptotic cell death as evidenced by cell viability, nuclear chromatin condensation, and apoptotic body formation measurements. These effects were mediated by the modulation of the mitochondrial function and of the HSP70 gene transcription and expression. Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and

Nad(p)h: quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2). Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

No MeSH data available.


Related in: MedlinePlus

Protective effect of broccoli sprouts juices on Aβ25–35-induced cytotoxicity. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 24, 48, and 72 h. Cell viability was determined by MTT reduction assay and expressed as percentage compared to control. Data are represented as mean ± SEM (n = 5), aP < 0.001 versus control; bP < 0.001 versus Aβ25–35; cP < 0.05 versus (A + Aβ).
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig1: Protective effect of broccoli sprouts juices on Aβ25–35-induced cytotoxicity. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 24, 48, and 72 h. Cell viability was determined by MTT reduction assay and expressed as percentage compared to control. Data are represented as mean ± SEM (n = 5), aP < 0.001 versus control; bP < 0.001 versus Aβ25–35; cP < 0.05 versus (A + Aβ).

Mentions: As shown in Figure 1, 25 μM Aβ25–35 treatment significantly decreased SH-SY5Y cells viability as compared to untreated cells (~70% at 24–48 h and 63% at 72 h, P < 0.001 versus control). The cotreatment with 10 μL/mL juice A or juice B significantly reduced Aβ25–35-induced cytotoxicity, restoring a cell viability comparable to that of the control (110% in (A + Aβ) and 117% in (B + Aβ) versus 71% in Aβ at 24 h, P < 0.001; 108% in (A + Aβ) and 113% in (B + Aβ) versus 69% in Aβ at 48 h, P < 0.001; 104% in (A + Aβ) and 102% in (B + Aβ) versus 63% in Aβ at 72 h, P < 0.001).


Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer's Disease.

Masci A, Mattioli R, Costantino P, Baima S, Morelli G, Punzi P, Giordano C, Pinto A, Donini LM, d'Erme M, Mosca L - Oxid Med Cell Longev (2015)

Protective effect of broccoli sprouts juices on Aβ25–35-induced cytotoxicity. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 24, 48, and 72 h. Cell viability was determined by MTT reduction assay and expressed as percentage compared to control. Data are represented as mean ± SEM (n = 5), aP < 0.001 versus control; bP < 0.001 versus Aβ25–35; cP < 0.05 versus (A + Aβ).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477226&req=5

fig1: Protective effect of broccoli sprouts juices on Aβ25–35-induced cytotoxicity. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 24, 48, and 72 h. Cell viability was determined by MTT reduction assay and expressed as percentage compared to control. Data are represented as mean ± SEM (n = 5), aP < 0.001 versus control; bP < 0.001 versus Aβ25–35; cP < 0.05 versus (A + Aβ).
Mentions: As shown in Figure 1, 25 μM Aβ25–35 treatment significantly decreased SH-SY5Y cells viability as compared to untreated cells (~70% at 24–48 h and 63% at 72 h, P < 0.001 versus control). The cotreatment with 10 μL/mL juice A or juice B significantly reduced Aβ25–35-induced cytotoxicity, restoring a cell viability comparable to that of the control (110% in (A + Aβ) and 117% in (B + Aβ) versus 71% in Aβ at 24 h, P < 0.001; 108% in (A + Aβ) and 113% in (B + Aβ) versus 69% in Aβ at 48 h, P < 0.001; 104% in (A + Aβ) and 102% in (B + Aβ) versus 63% in Aβ at 72 h, P < 0.001).

Bottom Line: In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions.Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2).Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine-Medical Physiopathology, Food Science and Endocrinology Section, Sapienza University, Rome, Italy.

ABSTRACT

Unlabelled: β-Amyloid peptide (Aβ) aberrant production and aggregation are major factors implicated in the pathogenesis of Alzheimer's disease (AD), causing neuronal death via oxidative stress. Several studies have highlighted the importance of polyphenolic antioxidant compounds in the treatment of AD, but complex food matrices, characterized by a different relative content of these phytochemicals, have been neglected. In the present study, we analyzed the protective effect on SH-SY5Y cells treated with the fragment Aβ 25-35 by two crude juices of broccoli sprouts containing different amounts of phenolic compounds as a result of different growth conditions. Both juices protected against Aβ-induced cytotoxicity and apoptotic cell death as evidenced by cell viability, nuclear chromatin condensation, and apoptotic body formation measurements. These effects were mediated by the modulation of the mitochondrial function and of the HSP70 gene transcription and expression. Furthermore, the juices upregulated the intracellular glutathione content and mRNA levels or activity of antioxidant enzymes such as heme oxygenase-1, thioredoxin, thioredoxin reductase, and

Nad(p)h: quinone oxidoreductase 1 via activation of NF-E2-related factor 2 (Nrf2). Although the effects of the two juices were similar, the juice enriched in phenolic compounds showed a greater efficacy in inducing the activation of the Nrf2 signalling pathway.

No MeSH data available.


Related in: MedlinePlus