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miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development.

Ribeiro J, Marinho-Dias J, Monteiro P, Loureiro J, Baldaque I, Medeiros R, Sousa H - Biomed Res Int (2015)

Bottom Line: For miR-125b, we observed a twofold (2(-ΔΔCt) = 2.11; P = 0.038) increased expression among women with normal epithelium with HPV infection and a trend of downregulation in different cervical lesions including an 80% reduction (2(-ΔΔCt) = 0.21; P = 0.004) in ICC.Similarly, miR-34a expression analysis revealed an increased expression (2(-ΔΔCt) = 1.69; P = 0.049) among women with normal cervix and HPV infection, and despite no significant correlation with cervical lesions, its expression was increased by twofold (2(-ΔΔCt) = 2.08; P = 0.042) in ICC.Moreover, miR-125b levels were able to predict invasive cancers with 88% sensitivity and 69% specificity.

View Article: PubMed Central - PubMed

Affiliation: Virology Service, Portuguese Institute of Oncology of Porto, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal ; Molecular Oncology and Viral Pathology Group (CI-IPOP), Portuguese Institute of Oncology of Porto, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal ; Faculty of Medicine, University of Porto (FMUP), Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal.

ABSTRACT
We aimed to characterize miR-125b and miR-34a expression in 114 women with different cervical lesions: normal epithelium with (n = 20) and without (n = 29) HPV infection; LSIL (n = 28); HSIL (n = 29); and ICC (n = 8). miRNA expression analysis was performed by comparing the distinct groups with the reference group (women with normal epithelium without HPV). For miR-125b, we observed a twofold (2(-ΔΔCt) = 2.11; P = 0.038) increased expression among women with normal epithelium with HPV infection and a trend of downregulation in different cervical lesions including an 80% reduction (2(-ΔΔCt) = 0.21; P = 0.004) in ICC. Similarly, miR-34a expression analysis revealed an increased expression (2(-ΔΔCt) = 1.69; P = 0.049) among women with normal cervix and HPV infection, and despite no significant correlation with cervical lesions, its expression was increased by twofold (2(-ΔΔCt) = 2.08; P = 0.042) in ICC. Moreover, miR-125b levels were able to predict invasive cancers with 88% sensitivity and 69% specificity. Results showed that while miR-34a expression seems to be correlated with invasive cervical cancer, miR-125b expression is significantly changed within the different cervical lesions and their levels should be further investigated as possible predictive/prognostic biomarkers using a noninvasive approach.

No MeSH data available.


Related in: MedlinePlus

Prediction of ICC development using miR-125b levels. AUC: area under the curve.
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fig2: Prediction of ICC development using miR-125b levels. AUC: area under the curve.

Mentions: Considering the results of miR-125b expression (Table 2) there are interesting data: firstly, we observed a twofold (2−ΔΔCt = 2.11; P = 0.038) increase in the group of women with normal cytology with HPV infection; then, despite not being statistically significant, we also found a decrease of miR-125b expression for LSIL or HSIL (2−ΔΔCt = 0.75 and 2−ΔΔCt = 0.55, resp.); and, finally, the analysis revealed that miR-125b expression was significantly decreased in women with ICC (2−ΔΔCt = 0.21; P = 0.004). The accuracy of miR-125b levels to discriminate between the different cervical lesions and normal cases was evaluated using ROC curves; nevertheless, significant results were only observed for ICC where the cut-off of −5.8 (ΔCt value) allows discriminating ICC with 88% sensitivity and 69% specificity (area under the curve = 0.802; P = 0.010) (Figure 2).


miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development.

Ribeiro J, Marinho-Dias J, Monteiro P, Loureiro J, Baldaque I, Medeiros R, Sousa H - Biomed Res Int (2015)

Prediction of ICC development using miR-125b levels. AUC: area under the curve.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477216&req=5

fig2: Prediction of ICC development using miR-125b levels. AUC: area under the curve.
Mentions: Considering the results of miR-125b expression (Table 2) there are interesting data: firstly, we observed a twofold (2−ΔΔCt = 2.11; P = 0.038) increase in the group of women with normal cytology with HPV infection; then, despite not being statistically significant, we also found a decrease of miR-125b expression for LSIL or HSIL (2−ΔΔCt = 0.75 and 2−ΔΔCt = 0.55, resp.); and, finally, the analysis revealed that miR-125b expression was significantly decreased in women with ICC (2−ΔΔCt = 0.21; P = 0.004). The accuracy of miR-125b levels to discriminate between the different cervical lesions and normal cases was evaluated using ROC curves; nevertheless, significant results were only observed for ICC where the cut-off of −5.8 (ΔCt value) allows discriminating ICC with 88% sensitivity and 69% specificity (area under the curve = 0.802; P = 0.010) (Figure 2).

Bottom Line: For miR-125b, we observed a twofold (2(-ΔΔCt) = 2.11; P = 0.038) increased expression among women with normal epithelium with HPV infection and a trend of downregulation in different cervical lesions including an 80% reduction (2(-ΔΔCt) = 0.21; P = 0.004) in ICC.Similarly, miR-34a expression analysis revealed an increased expression (2(-ΔΔCt) = 1.69; P = 0.049) among women with normal cervix and HPV infection, and despite no significant correlation with cervical lesions, its expression was increased by twofold (2(-ΔΔCt) = 2.08; P = 0.042) in ICC.Moreover, miR-125b levels were able to predict invasive cancers with 88% sensitivity and 69% specificity.

View Article: PubMed Central - PubMed

Affiliation: Virology Service, Portuguese Institute of Oncology of Porto, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal ; Molecular Oncology and Viral Pathology Group (CI-IPOP), Portuguese Institute of Oncology of Porto, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal ; Faculty of Medicine, University of Porto (FMUP), Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal.

ABSTRACT
We aimed to characterize miR-125b and miR-34a expression in 114 women with different cervical lesions: normal epithelium with (n = 20) and without (n = 29) HPV infection; LSIL (n = 28); HSIL (n = 29); and ICC (n = 8). miRNA expression analysis was performed by comparing the distinct groups with the reference group (women with normal epithelium without HPV). For miR-125b, we observed a twofold (2(-ΔΔCt) = 2.11; P = 0.038) increased expression among women with normal epithelium with HPV infection and a trend of downregulation in different cervical lesions including an 80% reduction (2(-ΔΔCt) = 0.21; P = 0.004) in ICC. Similarly, miR-34a expression analysis revealed an increased expression (2(-ΔΔCt) = 1.69; P = 0.049) among women with normal cervix and HPV infection, and despite no significant correlation with cervical lesions, its expression was increased by twofold (2(-ΔΔCt) = 2.08; P = 0.042) in ICC. Moreover, miR-125b levels were able to predict invasive cancers with 88% sensitivity and 69% specificity. Results showed that while miR-34a expression seems to be correlated with invasive cervical cancer, miR-125b expression is significantly changed within the different cervical lesions and their levels should be further investigated as possible predictive/prognostic biomarkers using a noninvasive approach.

No MeSH data available.


Related in: MedlinePlus