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Resveratrol Derivative-Rich Melinjo Seed Extract Attenuates Skin Atrophy in Sod1-Deficient Mice.

Watanabe K, Shibuya S, Ozawa Y, Izuo N, Shimizu T - Oxid Med Cell Longev (2015)

Bottom Line: Since the loss of cytoplasmic SOD (SOD1) resulted in aging-like phenotypes in several types of murine tissue, SOD1 is essential for the maintenance of tissue homeostasis.MSE intake also exerts no adverse events in human study.In vitro experiments revealed that RSV significantly promoted the viability of Sod1 (-/-) fibroblasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Advanced Aging Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.

ABSTRACT
The oxidative damages induced by a redox imbalance cause age-related changes in cells and tissues. Superoxide dismutase (SOD) enzymes play a pivotal role in the antioxidant system and they also catalyze superoxide radicals. Since the loss of cytoplasmic SOD (SOD1) resulted in aging-like phenotypes in several types of murine tissue, SOD1 is essential for the maintenance of tissue homeostasis. Melinjo (Gnetum gnemon Linn) seed extract (MSE) contains trans-resveratrol (RSV) and resveratrol derivatives, including gnetin C, gnemonoside A, and gnemonoside D. MSE intake also exerts no adverse events in human study. In the present studies, we investigated protective effects of MSE on age-related skin pathologies in mice. Orally MSE and RSV treatment reversed the skin thinning associated with increased oxidative damage in the Sod1 (-/-) mice. Furthermore, MSE and RSV normalized gene expression of Col1a1 and p53 and upregulated gene expression of Sirt1 in skin tissues. In vitro experiments revealed that RSV significantly promoted the viability of Sod1 (-/-) fibroblasts. These finding demonstrated that RSV in MSE stably suppressed an intrinsic superoxide generation in vivo and in vitro leading to protecting skin damages. RSV derivative-rich MSE may be a powerful food of treatment for age-related skin diseases caused by oxidative damages.

No MeSH data available.


Related in: MedlinePlus

MSE and RSV decrease oxidative damage and ROS production. (a) 8-isoprostane content in plasma obtained from Sod1−/− and Sod1+/+ mice treated with MSE and RSV (n = 10–12). (b) The intracellular ROS levels of bone marrow cells of Sod1−/− and Sod1+/+ mice were measured using a DCF dye (n = 5-6). (c) The relative intracellular ROS level in Sod1−/− fibroblasts treated with 10 μM RSV for 72 h was measured by a DCF dye (n = 3). The statistical evaluations were performed using the Tukey's test. These data indicate the mean ± SD; ∗P < 0.05, ∗∗P < 0.01.
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fig3: MSE and RSV decrease oxidative damage and ROS production. (a) 8-isoprostane content in plasma obtained from Sod1−/− and Sod1+/+ mice treated with MSE and RSV (n = 10–12). (b) The intracellular ROS levels of bone marrow cells of Sod1−/− and Sod1+/+ mice were measured using a DCF dye (n = 5-6). (c) The relative intracellular ROS level in Sod1−/− fibroblasts treated with 10 μM RSV for 72 h was measured by a DCF dye (n = 3). The statistical evaluations were performed using the Tukey's test. These data indicate the mean ± SD; ∗P < 0.05, ∗∗P < 0.01.

Mentions: Sod1−/− mice showed significant increase of several oxidative damage markers, including lipid peroxidation, in tissues [20, 24, 27, 28]. In order to evaluate oxidative damage, we measured the lipid peroxidation levels in the plasma. Regarding the 8-isoprostane levels, MSE and RSV containing diets significantly reduced the 8-isoprostane content in the plasma (Figure 3(a)). Furthermore, MSE and RSV containing diets decreased intracellular ROS level in cells from bone marrow (Figure 3(b)). These data indicate that MSE and RSV treatment mitigated oxidative damage in Sod1−/− mice.


Resveratrol Derivative-Rich Melinjo Seed Extract Attenuates Skin Atrophy in Sod1-Deficient Mice.

Watanabe K, Shibuya S, Ozawa Y, Izuo N, Shimizu T - Oxid Med Cell Longev (2015)

MSE and RSV decrease oxidative damage and ROS production. (a) 8-isoprostane content in plasma obtained from Sod1−/− and Sod1+/+ mice treated with MSE and RSV (n = 10–12). (b) The intracellular ROS levels of bone marrow cells of Sod1−/− and Sod1+/+ mice were measured using a DCF dye (n = 5-6). (c) The relative intracellular ROS level in Sod1−/− fibroblasts treated with 10 μM RSV for 72 h was measured by a DCF dye (n = 3). The statistical evaluations were performed using the Tukey's test. These data indicate the mean ± SD; ∗P < 0.05, ∗∗P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4477213&req=5

fig3: MSE and RSV decrease oxidative damage and ROS production. (a) 8-isoprostane content in plasma obtained from Sod1−/− and Sod1+/+ mice treated with MSE and RSV (n = 10–12). (b) The intracellular ROS levels of bone marrow cells of Sod1−/− and Sod1+/+ mice were measured using a DCF dye (n = 5-6). (c) The relative intracellular ROS level in Sod1−/− fibroblasts treated with 10 μM RSV for 72 h was measured by a DCF dye (n = 3). The statistical evaluations were performed using the Tukey's test. These data indicate the mean ± SD; ∗P < 0.05, ∗∗P < 0.01.
Mentions: Sod1−/− mice showed significant increase of several oxidative damage markers, including lipid peroxidation, in tissues [20, 24, 27, 28]. In order to evaluate oxidative damage, we measured the lipid peroxidation levels in the plasma. Regarding the 8-isoprostane levels, MSE and RSV containing diets significantly reduced the 8-isoprostane content in the plasma (Figure 3(a)). Furthermore, MSE and RSV containing diets decreased intracellular ROS level in cells from bone marrow (Figure 3(b)). These data indicate that MSE and RSV treatment mitigated oxidative damage in Sod1−/− mice.

Bottom Line: Since the loss of cytoplasmic SOD (SOD1) resulted in aging-like phenotypes in several types of murine tissue, SOD1 is essential for the maintenance of tissue homeostasis.MSE intake also exerts no adverse events in human study.In vitro experiments revealed that RSV significantly promoted the viability of Sod1 (-/-) fibroblasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Advanced Aging Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.

ABSTRACT
The oxidative damages induced by a redox imbalance cause age-related changes in cells and tissues. Superoxide dismutase (SOD) enzymes play a pivotal role in the antioxidant system and they also catalyze superoxide radicals. Since the loss of cytoplasmic SOD (SOD1) resulted in aging-like phenotypes in several types of murine tissue, SOD1 is essential for the maintenance of tissue homeostasis. Melinjo (Gnetum gnemon Linn) seed extract (MSE) contains trans-resveratrol (RSV) and resveratrol derivatives, including gnetin C, gnemonoside A, and gnemonoside D. MSE intake also exerts no adverse events in human study. In the present studies, we investigated protective effects of MSE on age-related skin pathologies in mice. Orally MSE and RSV treatment reversed the skin thinning associated with increased oxidative damage in the Sod1 (-/-) mice. Furthermore, MSE and RSV normalized gene expression of Col1a1 and p53 and upregulated gene expression of Sirt1 in skin tissues. In vitro experiments revealed that RSV significantly promoted the viability of Sod1 (-/-) fibroblasts. These finding demonstrated that RSV in MSE stably suppressed an intrinsic superoxide generation in vivo and in vitro leading to protecting skin damages. RSV derivative-rich MSE may be a powerful food of treatment for age-related skin diseases caused by oxidative damages.

No MeSH data available.


Related in: MedlinePlus