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HPV Testing from Dried Urine Spots as a Tool for Cervical Cancer Screening in Low-Income Countries.

Frati ER, Martinelli M, Fasoli E, Colzani D, Bianchi S, Binda S, Olivani P, Tanzi E - Biomed Res Int (2015)

Bottom Line: The specificity was 100% (95% CI: 89.28-100) at both time points.The concordance between DUS and fresh urine HPV testing was "almost perfect" using the κ statistic.These preliminary data suggest that a DUS-based assay could bypass sociocultural barriers and sampling issues and therefore could be a suitable, effective tool for epidemiological surveillance and screening programs, especially in low-income countries.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences for Health, University of Milan, 20133 Milan, Italy.

ABSTRACT
Nowadays, several screening strategies are available to prevent cervical cancer, but inadequate resources, sociocultural barriers, and sampling issues impede their success in low-income countries. To overcome these issues, this study aimed to evaluate the performance of human papillomavirus (HPV) testing from dried urine spots (DUS). Eighty-eight urine samples (including 56 HPV DNA positive specimens) were spotted on filter paper, dried, and stored in paper-bags. HPV DNA was detected from the DUS after 1 week and 4 weeks of storage using a polymerase chain reaction (PCR) assay. The sensitivity, specificity, and concordance of the DUS-based HPV test were evaluated by comparing the results with those of HPV testing on fresh urine samples as the gold standard. The sensitivity of the test was 98.21% (95% CI: 90.56-99.68) for DUS stored for 1 week and 96.42% (95% CI: 87.88-99.01) for DUS stored for 4 weeks. The specificity was 100% (95% CI: 89.28-100) at both time points. The concordance between DUS and fresh urine HPV testing was "almost perfect" using the κ statistic. These preliminary data suggest that a DUS-based assay could bypass sociocultural barriers and sampling issues and therefore could be a suitable, effective tool for epidemiological surveillance and screening programs, especially in low-income countries.

No MeSH data available.


Related in: MedlinePlus

Distribution of HPV genotypes in fresh urine samples and in corresponding DUS samples after four weeks of storage.
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Related In: Results  -  Collection


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fig1: Distribution of HPV genotypes in fresh urine samples and in corresponding DUS samples after four weeks of storage.

Mentions: Of the 40 DUS prepared from fresh urine samples of women infected by a single HPV genotype, 38 (95%) tested HPV DNA positive after four weeks of storage. All amplified fragments from the DUS were properly genotyped, and the distribution of HPV genotypes matched across the two sample types (Figure 1). The distribution of HR-clade and LR HPV genotypes was similar across the paired samples. In particular, 23 HPV DNA positive DUS were sustained by HR-clade genotypes and 15 were caused by LR genotypes.


HPV Testing from Dried Urine Spots as a Tool for Cervical Cancer Screening in Low-Income Countries.

Frati ER, Martinelli M, Fasoli E, Colzani D, Bianchi S, Binda S, Olivani P, Tanzi E - Biomed Res Int (2015)

Distribution of HPV genotypes in fresh urine samples and in corresponding DUS samples after four weeks of storage.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477205&req=5

fig1: Distribution of HPV genotypes in fresh urine samples and in corresponding DUS samples after four weeks of storage.
Mentions: Of the 40 DUS prepared from fresh urine samples of women infected by a single HPV genotype, 38 (95%) tested HPV DNA positive after four weeks of storage. All amplified fragments from the DUS were properly genotyped, and the distribution of HPV genotypes matched across the two sample types (Figure 1). The distribution of HR-clade and LR HPV genotypes was similar across the paired samples. In particular, 23 HPV DNA positive DUS were sustained by HR-clade genotypes and 15 were caused by LR genotypes.

Bottom Line: The specificity was 100% (95% CI: 89.28-100) at both time points.The concordance between DUS and fresh urine HPV testing was "almost perfect" using the κ statistic.These preliminary data suggest that a DUS-based assay could bypass sociocultural barriers and sampling issues and therefore could be a suitable, effective tool for epidemiological surveillance and screening programs, especially in low-income countries.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences for Health, University of Milan, 20133 Milan, Italy.

ABSTRACT
Nowadays, several screening strategies are available to prevent cervical cancer, but inadequate resources, sociocultural barriers, and sampling issues impede their success in low-income countries. To overcome these issues, this study aimed to evaluate the performance of human papillomavirus (HPV) testing from dried urine spots (DUS). Eighty-eight urine samples (including 56 HPV DNA positive specimens) were spotted on filter paper, dried, and stored in paper-bags. HPV DNA was detected from the DUS after 1 week and 4 weeks of storage using a polymerase chain reaction (PCR) assay. The sensitivity, specificity, and concordance of the DUS-based HPV test were evaluated by comparing the results with those of HPV testing on fresh urine samples as the gold standard. The sensitivity of the test was 98.21% (95% CI: 90.56-99.68) for DUS stored for 1 week and 96.42% (95% CI: 87.88-99.01) for DUS stored for 4 weeks. The specificity was 100% (95% CI: 89.28-100) at both time points. The concordance between DUS and fresh urine HPV testing was "almost perfect" using the κ statistic. These preliminary data suggest that a DUS-based assay could bypass sociocultural barriers and sampling issues and therefore could be a suitable, effective tool for epidemiological surveillance and screening programs, especially in low-income countries.

No MeSH data available.


Related in: MedlinePlus