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Inter-individual stereotypy of the Platynereis larval visual connectome.

Randel N, Shahidi R, Verasztó C, Bezares-Calderón LA, Schmidt S, Jékely G - Elife (2015)

Bottom Line: It is less well understood, however, to what extent entire neural circuits of different individuals are similar.Previously, we reported the neuronal connectome of the visual eye circuit from the head of a Platynereis dumerilii larva (Randel et al., 2014).Our work shows the extent to which the eye circuitry in Platynereis larvae is hard-wired.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Developmental Biology, Tübingen, Germany.

ABSTRACT
Developmental programs have the fidelity to form neural circuits with the same structure and function among individuals of the same species. It is less well understood, however, to what extent entire neural circuits of different individuals are similar. Previously, we reported the neuronal connectome of the visual eye circuit from the head of a Platynereis dumerilii larva (Randel et al., 2014). We now report a full-body serial section transmission electron microscopy (ssTEM) dataset of another larva of the same age, for which we describe the connectome of the visual eyes and the larval eyespots. Anatomical comparisons and quantitative analyses of the two circuits reveal a high inter-individual stereotypy of the cell complement, neuronal projections, and synaptic connectivity, including the left-right asymmetry in the connectivity of some neurons. Our work shows the extent to which the eye circuitry in Platynereis larvae is hard-wired.

No MeSH data available.


Related in: MedlinePlus

Full connectivity matrix of the visual and larval eye circuit of the fully reconstructed larva HT9-4.DOI:http://dx.doi.org/10.7554/eLife.08069.004
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fig1s1: Full connectivity matrix of the visual and larval eye circuit of the fully reconstructed larva HT9-4.DOI:http://dx.doi.org/10.7554/eLife.08069.004

Mentions: To test the stereotypy of synaptic connections, we compared patterns of connectivity between the two individuals (Figure 1B,C, Figure 2B, Figure 1—figure supplement 1, Figure 2—source data 1). We used several measures to compare the two connectomes. First, we defined a grouped connectivity matrix for HT9-4, similar to that of HT9-3 (Randel et al., 2014) (Figure 2—source data 1). We then correlated the two grouped matrices and found a strong correlation (Spearman's r = 0.62, p = 0.0001). For a more detailed comparison, we calculated the geometric mean of the two connectivity matrices and generated a combined matrix (Figure 2A). In this matrix, only connections are shown that are present in both animals. The combined matrix is similar to the individual matrices and we could find all major connections.10.7554/eLife.08069.006Figure 2.Comparative analysis of HT9-3 and HT9-4 visual eye circuits.


Inter-individual stereotypy of the Platynereis larval visual connectome.

Randel N, Shahidi R, Verasztó C, Bezares-Calderón LA, Schmidt S, Jékely G - Elife (2015)

Full connectivity matrix of the visual and larval eye circuit of the fully reconstructed larva HT9-4.DOI:http://dx.doi.org/10.7554/eLife.08069.004
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477197&req=5

fig1s1: Full connectivity matrix of the visual and larval eye circuit of the fully reconstructed larva HT9-4.DOI:http://dx.doi.org/10.7554/eLife.08069.004
Mentions: To test the stereotypy of synaptic connections, we compared patterns of connectivity between the two individuals (Figure 1B,C, Figure 2B, Figure 1—figure supplement 1, Figure 2—source data 1). We used several measures to compare the two connectomes. First, we defined a grouped connectivity matrix for HT9-4, similar to that of HT9-3 (Randel et al., 2014) (Figure 2—source data 1). We then correlated the two grouped matrices and found a strong correlation (Spearman's r = 0.62, p = 0.0001). For a more detailed comparison, we calculated the geometric mean of the two connectivity matrices and generated a combined matrix (Figure 2A). In this matrix, only connections are shown that are present in both animals. The combined matrix is similar to the individual matrices and we could find all major connections.10.7554/eLife.08069.006Figure 2.Comparative analysis of HT9-3 and HT9-4 visual eye circuits.

Bottom Line: It is less well understood, however, to what extent entire neural circuits of different individuals are similar.Previously, we reported the neuronal connectome of the visual eye circuit from the head of a Platynereis dumerilii larva (Randel et al., 2014).Our work shows the extent to which the eye circuitry in Platynereis larvae is hard-wired.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Developmental Biology, Tübingen, Germany.

ABSTRACT
Developmental programs have the fidelity to form neural circuits with the same structure and function among individuals of the same species. It is less well understood, however, to what extent entire neural circuits of different individuals are similar. Previously, we reported the neuronal connectome of the visual eye circuit from the head of a Platynereis dumerilii larva (Randel et al., 2014). We now report a full-body serial section transmission electron microscopy (ssTEM) dataset of another larva of the same age, for which we describe the connectome of the visual eyes and the larval eyespots. Anatomical comparisons and quantitative analyses of the two circuits reveal a high inter-individual stereotypy of the cell complement, neuronal projections, and synaptic connectivity, including the left-right asymmetry in the connectivity of some neurons. Our work shows the extent to which the eye circuitry in Platynereis larvae is hard-wired.

No MeSH data available.


Related in: MedlinePlus