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Inter-individual stereotypy of the Platynereis larval visual connectome.

Randel N, Shahidi R, Verasztó C, Bezares-Calderón LA, Schmidt S, Jékely G - Elife (2015)

Bottom Line: It is less well understood, however, to what extent entire neural circuits of different individuals are similar.Previously, we reported the neuronal connectome of the visual eye circuit from the head of a Platynereis dumerilii larva (Randel et al., 2014).Our work shows the extent to which the eye circuitry in Platynereis larvae is hard-wired.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Developmental Biology, Tübingen, Germany.

ABSTRACT
Developmental programs have the fidelity to form neural circuits with the same structure and function among individuals of the same species. It is less well understood, however, to what extent entire neural circuits of different individuals are similar. Previously, we reported the neuronal connectome of the visual eye circuit from the head of a Platynereis dumerilii larva (Randel et al., 2014). We now report a full-body serial section transmission electron microscopy (ssTEM) dataset of another larva of the same age, for which we describe the connectome of the visual eyes and the larval eyespots. Anatomical comparisons and quantitative analyses of the two circuits reveal a high inter-individual stereotypy of the cell complement, neuronal projections, and synaptic connectivity, including the left-right asymmetry in the connectivity of some neurons. Our work shows the extent to which the eye circuitry in Platynereis larvae is hard-wired.

No MeSH data available.


Related in: MedlinePlus

Minimal eye circuit of the larval eye.Neurons that are also part of the visual eye circuit are marked by an ‘asterisk’. Connectivity graph of the r-opsin3-expressing PRCr3 (A) and r-opsin1-expressing PRCr1 (C). Connectivity graph edges are weighted by the number of synapses and synapse numbers are shown. Blender visualization of the left PRCr3l cell (B) and PRCr1r circuitry (D). Abbreviations are shown in Figure 1.DOI:http://dx.doi.org/10.7554/eLife.08069.013
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fig4: Minimal eye circuit of the larval eye.Neurons that are also part of the visual eye circuit are marked by an ‘asterisk’. Connectivity graph of the r-opsin3-expressing PRCr3 (A) and r-opsin1-expressing PRCr1 (C). Connectivity graph edges are weighted by the number of synapses and synapse numbers are shown. Blender visualization of the left PRCr3l cell (B) and PRCr1r circuitry (D). Abbreviations are shown in Figure 1.DOI:http://dx.doi.org/10.7554/eLife.08069.013

Mentions: We also fully reconstructed the neuronal circuitry of the larval eyespots or ocelli (structures located ventrally in the head) in HT9-4 (Figure 4). The larval eyespots develop in early trochophore larval stages and mediate positive phototaxis in early larval stages by innervating adjacent ciliary band cells (Jékely et al., 2008). In trochophore larvae (24–48 hpf), each larval eyespot consists of one pigment cell and one PRCr3, the latter of which expresses a rhabdomeric opsin, r-opsin3. A preliminary ssTEM analysis in HT9-3 (72 hpf) identified a second PRCr1 with an axon projecting towards the brain neuropil (a cerebral PRC), indicating changes in eyespot structure during development. The second PRC expresses another opsin, r-opsin1 (Randel et al., 2013).10.7554/eLife.08069.013Figure 4.Minimal eye circuit of the larval eye.


Inter-individual stereotypy of the Platynereis larval visual connectome.

Randel N, Shahidi R, Verasztó C, Bezares-Calderón LA, Schmidt S, Jékely G - Elife (2015)

Minimal eye circuit of the larval eye.Neurons that are also part of the visual eye circuit are marked by an ‘asterisk’. Connectivity graph of the r-opsin3-expressing PRCr3 (A) and r-opsin1-expressing PRCr1 (C). Connectivity graph edges are weighted by the number of synapses and synapse numbers are shown. Blender visualization of the left PRCr3l cell (B) and PRCr1r circuitry (D). Abbreviations are shown in Figure 1.DOI:http://dx.doi.org/10.7554/eLife.08069.013
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4477197&req=5

fig4: Minimal eye circuit of the larval eye.Neurons that are also part of the visual eye circuit are marked by an ‘asterisk’. Connectivity graph of the r-opsin3-expressing PRCr3 (A) and r-opsin1-expressing PRCr1 (C). Connectivity graph edges are weighted by the number of synapses and synapse numbers are shown. Blender visualization of the left PRCr3l cell (B) and PRCr1r circuitry (D). Abbreviations are shown in Figure 1.DOI:http://dx.doi.org/10.7554/eLife.08069.013
Mentions: We also fully reconstructed the neuronal circuitry of the larval eyespots or ocelli (structures located ventrally in the head) in HT9-4 (Figure 4). The larval eyespots develop in early trochophore larval stages and mediate positive phototaxis in early larval stages by innervating adjacent ciliary band cells (Jékely et al., 2008). In trochophore larvae (24–48 hpf), each larval eyespot consists of one pigment cell and one PRCr3, the latter of which expresses a rhabdomeric opsin, r-opsin3. A preliminary ssTEM analysis in HT9-3 (72 hpf) identified a second PRCr1 with an axon projecting towards the brain neuropil (a cerebral PRC), indicating changes in eyespot structure during development. The second PRC expresses another opsin, r-opsin1 (Randel et al., 2013).10.7554/eLife.08069.013Figure 4.Minimal eye circuit of the larval eye.

Bottom Line: It is less well understood, however, to what extent entire neural circuits of different individuals are similar.Previously, we reported the neuronal connectome of the visual eye circuit from the head of a Platynereis dumerilii larva (Randel et al., 2014).Our work shows the extent to which the eye circuitry in Platynereis larvae is hard-wired.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Developmental Biology, Tübingen, Germany.

ABSTRACT
Developmental programs have the fidelity to form neural circuits with the same structure and function among individuals of the same species. It is less well understood, however, to what extent entire neural circuits of different individuals are similar. Previously, we reported the neuronal connectome of the visual eye circuit from the head of a Platynereis dumerilii larva (Randel et al., 2014). We now report a full-body serial section transmission electron microscopy (ssTEM) dataset of another larva of the same age, for which we describe the connectome of the visual eyes and the larval eyespots. Anatomical comparisons and quantitative analyses of the two circuits reveal a high inter-individual stereotypy of the cell complement, neuronal projections, and synaptic connectivity, including the left-right asymmetry in the connectivity of some neurons. Our work shows the extent to which the eye circuitry in Platynereis larvae is hard-wired.

No MeSH data available.


Related in: MedlinePlus