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Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD - Oxid Med Cell Longev (2015)

Bottom Line: Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection.Lowbush blueberry extract triggers the strongest activity.We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.

ABSTRACT

Scope: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

Methods and results: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

Conclusion: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

No MeSH data available.


Related in: MedlinePlus

Effect of lowbush blueberry proanthocyanidins (Pcys) (50 μg/mL) on the density of death receptors present at the cell surface of SW620 (a) and SW480 (b) cells. TRAIL-R1 receptor is specifically recognized by a monoclonal antibody Ac1 coupled to Alexa 488 (which emits at 525 nm) whereas TRAIL-R2 receptor is specifically recognized by a monoclonal antibody Ac2 coupled to phycoerythrin (which emits at 575 nm). Fas receptor is specifically recognized by a monoclonal antibody Ac3 coupled to phycoerythrin-Cy5 (PE-Cy5) (which emits at 670 nm). TRAIL-R1 receptor presence is materialized by the mean green fluorescence emitted by Ac1 antibody on SW620 or SW480 cell lines, while TRAIL-R2 receptor is materialized by the mean yellow fluorescence emitted by Ac2 antibody and Fas receptor is materialized by the red green fluorescence emitted by Ac3 antibody. Statistical significant differences (n = 3 independent experiments) based on mean fluorescence (AU) of the cell population between labeled cells in absence of proanthocyanidins and cells labeled and treated with Pcys are represented by the symbol “∗.”
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Related In: Results  -  Collection


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fig5: Effect of lowbush blueberry proanthocyanidins (Pcys) (50 μg/mL) on the density of death receptors present at the cell surface of SW620 (a) and SW480 (b) cells. TRAIL-R1 receptor is specifically recognized by a monoclonal antibody Ac1 coupled to Alexa 488 (which emits at 525 nm) whereas TRAIL-R2 receptor is specifically recognized by a monoclonal antibody Ac2 coupled to phycoerythrin (which emits at 575 nm). Fas receptor is specifically recognized by a monoclonal antibody Ac3 coupled to phycoerythrin-Cy5 (PE-Cy5) (which emits at 670 nm). TRAIL-R1 receptor presence is materialized by the mean green fluorescence emitted by Ac1 antibody on SW620 or SW480 cell lines, while TRAIL-R2 receptor is materialized by the mean yellow fluorescence emitted by Ac2 antibody and Fas receptor is materialized by the red green fluorescence emitted by Ac3 antibody. Statistical significant differences (n = 3 independent experiments) based on mean fluorescence (AU) of the cell population between labeled cells in absence of proanthocyanidins and cells labeled and treated with Pcys are represented by the symbol “∗.”

Mentions: Lowbush blueberry Pcys did not modify TRAIL-R1 or TRAIL-R2 (Figure 4) nor Fas receptor expression (Figure 5). Only a slight decrease could be noticed for TRAIL-R2 and Fas in SW480 cell line.


Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD - Oxid Med Cell Longev (2015)

Effect of lowbush blueberry proanthocyanidins (Pcys) (50 μg/mL) on the density of death receptors present at the cell surface of SW620 (a) and SW480 (b) cells. TRAIL-R1 receptor is specifically recognized by a monoclonal antibody Ac1 coupled to Alexa 488 (which emits at 525 nm) whereas TRAIL-R2 receptor is specifically recognized by a monoclonal antibody Ac2 coupled to phycoerythrin (which emits at 575 nm). Fas receptor is specifically recognized by a monoclonal antibody Ac3 coupled to phycoerythrin-Cy5 (PE-Cy5) (which emits at 670 nm). TRAIL-R1 receptor presence is materialized by the mean green fluorescence emitted by Ac1 antibody on SW620 or SW480 cell lines, while TRAIL-R2 receptor is materialized by the mean yellow fluorescence emitted by Ac2 antibody and Fas receptor is materialized by the red green fluorescence emitted by Ac3 antibody. Statistical significant differences (n = 3 independent experiments) based on mean fluorescence (AU) of the cell population between labeled cells in absence of proanthocyanidins and cells labeled and treated with Pcys are represented by the symbol “∗.”
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477188&req=5

fig5: Effect of lowbush blueberry proanthocyanidins (Pcys) (50 μg/mL) on the density of death receptors present at the cell surface of SW620 (a) and SW480 (b) cells. TRAIL-R1 receptor is specifically recognized by a monoclonal antibody Ac1 coupled to Alexa 488 (which emits at 525 nm) whereas TRAIL-R2 receptor is specifically recognized by a monoclonal antibody Ac2 coupled to phycoerythrin (which emits at 575 nm). Fas receptor is specifically recognized by a monoclonal antibody Ac3 coupled to phycoerythrin-Cy5 (PE-Cy5) (which emits at 670 nm). TRAIL-R1 receptor presence is materialized by the mean green fluorescence emitted by Ac1 antibody on SW620 or SW480 cell lines, while TRAIL-R2 receptor is materialized by the mean yellow fluorescence emitted by Ac2 antibody and Fas receptor is materialized by the red green fluorescence emitted by Ac3 antibody. Statistical significant differences (n = 3 independent experiments) based on mean fluorescence (AU) of the cell population between labeled cells in absence of proanthocyanidins and cells labeled and treated with Pcys are represented by the symbol “∗.”
Mentions: Lowbush blueberry Pcys did not modify TRAIL-R1 or TRAIL-R2 (Figure 4) nor Fas receptor expression (Figure 5). Only a slight decrease could be noticed for TRAIL-R2 and Fas in SW480 cell line.

Bottom Line: Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection.Lowbush blueberry extract triggers the strongest activity.We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.

ABSTRACT

Scope: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

Methods and results: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

Conclusion: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

No MeSH data available.


Related in: MedlinePlus