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Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD - Oxid Med Cell Longev (2015)

Bottom Line: Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection.Lowbush blueberry extract triggers the strongest activity.We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.

ABSTRACT

Scope: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

Methods and results: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

Conclusion: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

No MeSH data available.


Related in: MedlinePlus

Effect of lowbush blueberry Pcy on TRAIL-mediated apoptosis in SW480 and SW620 cells. The nonsynergistical effects of TRAIL (100 ng/mL) and lowbush blueberry proanthocyanidins (Pcys) (50 μg/mL) combination on apoptosis of SW620 (a) and SW480 (b) cell lines are illustrated by the dashed area reporting TRAIL alone activation and overlaid on the data obtained for TRAIL + Pcy.
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fig4: Effect of lowbush blueberry Pcy on TRAIL-mediated apoptosis in SW480 and SW620 cells. The nonsynergistical effects of TRAIL (100 ng/mL) and lowbush blueberry proanthocyanidins (Pcys) (50 μg/mL) combination on apoptosis of SW620 (a) and SW480 (b) cell lines are illustrated by the dashed area reporting TRAIL alone activation and overlaid on the data obtained for TRAIL + Pcy.

Mentions: Lowbush blueberry Pcys did not modify TRAIL-R1 or TRAIL-R2 (Figure 4) nor Fas receptor expression (Figure 5). Only a slight decrease could be noticed for TRAIL-R2 and Fas in SW480 cell line.


Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD - Oxid Med Cell Longev (2015)

Effect of lowbush blueberry Pcy on TRAIL-mediated apoptosis in SW480 and SW620 cells. The nonsynergistical effects of TRAIL (100 ng/mL) and lowbush blueberry proanthocyanidins (Pcys) (50 μg/mL) combination on apoptosis of SW620 (a) and SW480 (b) cell lines are illustrated by the dashed area reporting TRAIL alone activation and overlaid on the data obtained for TRAIL + Pcy.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477188&req=5

fig4: Effect of lowbush blueberry Pcy on TRAIL-mediated apoptosis in SW480 and SW620 cells. The nonsynergistical effects of TRAIL (100 ng/mL) and lowbush blueberry proanthocyanidins (Pcys) (50 μg/mL) combination on apoptosis of SW620 (a) and SW480 (b) cell lines are illustrated by the dashed area reporting TRAIL alone activation and overlaid on the data obtained for TRAIL + Pcy.
Mentions: Lowbush blueberry Pcys did not modify TRAIL-R1 or TRAIL-R2 (Figure 4) nor Fas receptor expression (Figure 5). Only a slight decrease could be noticed for TRAIL-R2 and Fas in SW480 cell line.

Bottom Line: Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection.Lowbush blueberry extract triggers the strongest activity.We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.

ABSTRACT

Scope: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

Methods and results: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

Conclusion: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

No MeSH data available.


Related in: MedlinePlus