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Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD - Oxid Med Cell Longev (2015)

Bottom Line: Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection.Lowbush blueberry extract triggers the strongest activity.We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.

ABSTRACT

Scope: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

Methods and results: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

Conclusion: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

No MeSH data available.


Related in: MedlinePlus

PS exposure on THP-1 monocytic cells induced by lowbush blueberry Pcys. Dose-response curve and EC50 value for lowbush blueberry Pcys incubated in raising concentrations were determined on THP-1 cells (upper right graph) after 24 h of incubation. Cells were stained using Annexin V-/PI apoptotic assay as described in Section 2.3 (n = 3 independent experiments; 2,000 events per sample were analyzed). Cytograms presented here illustrate the fact that Pcys on early apoptosis could be observed on the contrary of the data obtained for 50 μM celastrol (lower right cytogram).
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fig3: PS exposure on THP-1 monocytic cells induced by lowbush blueberry Pcys. Dose-response curve and EC50 value for lowbush blueberry Pcys incubated in raising concentrations were determined on THP-1 cells (upper right graph) after 24 h of incubation. Cells were stained using Annexin V-/PI apoptotic assay as described in Section 2.3 (n = 3 independent experiments; 2,000 events per sample were analyzed). Cytograms presented here illustrate the fact that Pcys on early apoptosis could be observed on the contrary of the data obtained for 50 μM celastrol (lower right cytogram).

Mentions: At around 100 μg/mL Pcys, 50% of the cells exhibited PS on the cell surface (early apoptosis) when at the same time (Figure 3) celastrol (50 μM) induced 97% of cells in late apoptosis. At 100 μg/mL of fruit Pcys, Chacón et al. reported a percentage of viability >97% when monitoring LDH [51]. In the same manner after 24H of treatment, no increase in number of hypodiploid sub-G1 cells could be observed when monocytic cells THP-1 were exposed to 50 μg/mL of lowbush blueberry Pcys (Table 3) suggesting no apoptotic activity in these cells. Taken together these data suggest an important cell surface disturbant activity of Pcys on THP-1 inflammatory cells without leading to an apoptotic cell death.


Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD - Oxid Med Cell Longev (2015)

PS exposure on THP-1 monocytic cells induced by lowbush blueberry Pcys. Dose-response curve and EC50 value for lowbush blueberry Pcys incubated in raising concentrations were determined on THP-1 cells (upper right graph) after 24 h of incubation. Cells were stained using Annexin V-/PI apoptotic assay as described in Section 2.3 (n = 3 independent experiments; 2,000 events per sample were analyzed). Cytograms presented here illustrate the fact that Pcys on early apoptosis could be observed on the contrary of the data obtained for 50 μM celastrol (lower right cytogram).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477188&req=5

fig3: PS exposure on THP-1 monocytic cells induced by lowbush blueberry Pcys. Dose-response curve and EC50 value for lowbush blueberry Pcys incubated in raising concentrations were determined on THP-1 cells (upper right graph) after 24 h of incubation. Cells were stained using Annexin V-/PI apoptotic assay as described in Section 2.3 (n = 3 independent experiments; 2,000 events per sample were analyzed). Cytograms presented here illustrate the fact that Pcys on early apoptosis could be observed on the contrary of the data obtained for 50 μM celastrol (lower right cytogram).
Mentions: At around 100 μg/mL Pcys, 50% of the cells exhibited PS on the cell surface (early apoptosis) when at the same time (Figure 3) celastrol (50 μM) induced 97% of cells in late apoptosis. At 100 μg/mL of fruit Pcys, Chacón et al. reported a percentage of viability >97% when monitoring LDH [51]. In the same manner after 24H of treatment, no increase in number of hypodiploid sub-G1 cells could be observed when monocytic cells THP-1 were exposed to 50 μg/mL of lowbush blueberry Pcys (Table 3) suggesting no apoptotic activity in these cells. Taken together these data suggest an important cell surface disturbant activity of Pcys on THP-1 inflammatory cells without leading to an apoptotic cell death.

Bottom Line: Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection.Lowbush blueberry extract triggers the strongest activity.We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.

ABSTRACT

Scope: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

Methods and results: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

Conclusion: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

No MeSH data available.


Related in: MedlinePlus