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Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD - Oxid Med Cell Longev (2015)

Bottom Line: Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection.Lowbush blueberry extract triggers the strongest activity.We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.

ABSTRACT

Scope: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

Methods and results: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

Conclusion: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

No MeSH data available.


Related in: MedlinePlus

EC50 of proapoptotic activities. Dose effect curves were plotted for apple Pcy (- - -), proanthocyanidin-rich extracts obtained from lowbush blueberry (—), and lingonberry (··-··-) when tested on SW620 (a) or SW480 (b) cell lines.
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Related In: Results  -  Collection


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fig2: EC50 of proapoptotic activities. Dose effect curves were plotted for apple Pcy (- - -), proanthocyanidin-rich extracts obtained from lowbush blueberry (—), and lingonberry (··-··-) when tested on SW620 (a) or SW480 (b) cell lines.

Mentions: These extracts were tested for their proapoptotic activities on SW480 and SW620 cell lines at several concentrations. Dose-response curves were computed to obtain EC50 values (Figure 2) with noteworthy differences between cell lines or Pcy origins. One should notice that only lowbush blueberry Pcys were able to induce more than 90% of apoptosis on both cell lines (92–95%), followed by apple Pcys (55–64%) and finally by lingonberry Pcys (37–41%) as shown in Table 2.


Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD - Oxid Med Cell Longev (2015)

EC50 of proapoptotic activities. Dose effect curves were plotted for apple Pcy (- - -), proanthocyanidin-rich extracts obtained from lowbush blueberry (—), and lingonberry (··-··-) when tested on SW620 (a) or SW480 (b) cell lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477188&req=5

fig2: EC50 of proapoptotic activities. Dose effect curves were plotted for apple Pcy (- - -), proanthocyanidin-rich extracts obtained from lowbush blueberry (—), and lingonberry (··-··-) when tested on SW620 (a) or SW480 (b) cell lines.
Mentions: These extracts were tested for their proapoptotic activities on SW480 and SW620 cell lines at several concentrations. Dose-response curves were computed to obtain EC50 values (Figure 2) with noteworthy differences between cell lines or Pcy origins. One should notice that only lowbush blueberry Pcys were able to induce more than 90% of apoptosis on both cell lines (92–95%), followed by apple Pcys (55–64%) and finally by lingonberry Pcys (37–41%) as shown in Table 2.

Bottom Line: Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection.Lowbush blueberry extract triggers the strongest activity.We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.

ABSTRACT

Scope: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

Methods and results: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

Conclusion: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

No MeSH data available.


Related in: MedlinePlus